Downregulation of long non-coding RNA B-Raf proto-oncogene-activated non-coding RNA reverses cisplatin resistance in laryngeal squamous cell carcinoma
Introduction This study was performed to explore the function of B-Raf proto-oncogene-activated non-coding RNA (BANCR) in laryngeal squamous cell carcinoma (LSCC) and cisplatin resistance. Material and methods The relative expression level of long non-coding RNA (lncRNA) BANCR was examined by qRT-P...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2019-12-01
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| Series: | Archives of Medical Science |
| Subjects: | |
| Online Access: | https://www.archivesofmedicalscience.com/Downregulation-of-long-non-coding-RNA-B-Raf-proto-oncogene-activated-non-coding-RNA,98949,0,2.html |
| _version_ | 1811181165211549696 |
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| author | Weiwei Han Lin Niu Lin Wang Jixiang Liu Huanying Li |
| author_facet | Weiwei Han Lin Niu Lin Wang Jixiang Liu Huanying Li |
| author_sort | Weiwei Han |
| collection | DOAJ |
| description | Introduction
This study was performed to explore the function of B-Raf proto-oncogene-activated non-coding RNA (BANCR) in laryngeal squamous cell carcinoma (LSCC) and cisplatin resistance.
Material and methods
The relative expression level of long non-coding RNA (lncRNA) BANCR was examined by qRT-PCR in tumor tissues and adjacent tissues, normal laryngeal cells (Het-1A) and laryngeal squamous carcinoma cells (TU686, TU177). Cisplatin-resistant laryngeal squamous carcinoma cell lines (TU686-DDP-R, TU177-DDP-R) were established. Next, we inhibited BANCR expression by transfecting siRNA-BANCR and enhanced BANCR expression by transfecting pcDNA3.1-BANCR into TU686, TU177, TU686-DDP-R and TU177-DDP-R cells. The CCK-8 assay and clone formation assay were performed to detect colony proliferation ability and formation ability of cells. Further, to investigate through which BANCR cell viability/formation is regulated, we detected the expression of MRP1, Bcl-2, p-PKB, and Bax by western blot.
Results
BANCR was highly expressed in laryngeal squamous carcinoma tissues and cells. Chemoresistance was generated in TU686-DDP-R and
TU177-DDP-R compared with TU686 and TU177 cells after cisplatin treatment. In addition, upregulated lncRNA BANCR reduced or postponed cell sensitivity to cisplatin by enhancing cell proliferation in TU686 and TU177 cells. Meanwhile, the expression of MRP1, Bcl-2, and p-PKB was increased, while Bax was reduced. After cisplatin treatment, down-regulation of BANCR could consequently attenuate TU686-DDP-R and TU177-DDP-R cell proliferation, and the expression of MRP1, Bcl-2, and p-PKB was decreased and Bax was increased.
Conclusions
Down-regulation of BANCR reverses cisplatin resistance of cisplatin-resistant LSCC cell lines. |
| first_indexed | 2024-04-11T09:14:56Z |
| format | Article |
| id | doaj.art-614d10528b4745e8ba320e9a3f6a2bd2 |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-04-11T09:14:56Z |
| publishDate | 2019-12-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-614d10528b4745e8ba320e9a3f6a2bd22022-12-22T04:32:23ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512019-12-011751164117410.5114/aoms.2019.9135298949Downregulation of long non-coding RNA B-Raf proto-oncogene-activated non-coding RNA reverses cisplatin resistance in laryngeal squamous cell carcinomaWeiwei Han0Lin Niu1Lin Wang2Jixiang Liu3Huanying Li4Department of Otolaryngology Head and Neck Surgery, Tianjin Union Medicine Centre, Tianjin, ChinaDepartment of Otolaryngology Head and Neck Surgery, Tianjin Union Medicine Centre, Tianjin, ChinaDepartment of Otolaryngology Head and Neck Surgery, Tianjin Union Medicine Centre, Tianjin, ChinaDepartment of Otolaryngology Head and Neck Surgery, Tianjin Union Medicine Centre, Tianjin, ChinaDepartment of Otolaryngology Head and Neck Surgery, Tianjin Union Medicine Centre, Tianjin, ChinaIntroduction This study was performed to explore the function of B-Raf proto-oncogene-activated non-coding RNA (BANCR) in laryngeal squamous cell carcinoma (LSCC) and cisplatin resistance. Material and methods The relative expression level of long non-coding RNA (lncRNA) BANCR was examined by qRT-PCR in tumor tissues and adjacent tissues, normal laryngeal cells (Het-1A) and laryngeal squamous carcinoma cells (TU686, TU177). Cisplatin-resistant laryngeal squamous carcinoma cell lines (TU686-DDP-R, TU177-DDP-R) were established. Next, we inhibited BANCR expression by transfecting siRNA-BANCR and enhanced BANCR expression by transfecting pcDNA3.1-BANCR into TU686, TU177, TU686-DDP-R and TU177-DDP-R cells. The CCK-8 assay and clone formation assay were performed to detect colony proliferation ability and formation ability of cells. Further, to investigate through which BANCR cell viability/formation is regulated, we detected the expression of MRP1, Bcl-2, p-PKB, and Bax by western blot. Results BANCR was highly expressed in laryngeal squamous carcinoma tissues and cells. Chemoresistance was generated in TU686-DDP-R and TU177-DDP-R compared with TU686 and TU177 cells after cisplatin treatment. In addition, upregulated lncRNA BANCR reduced or postponed cell sensitivity to cisplatin by enhancing cell proliferation in TU686 and TU177 cells. Meanwhile, the expression of MRP1, Bcl-2, and p-PKB was increased, while Bax was reduced. After cisplatin treatment, down-regulation of BANCR could consequently attenuate TU686-DDP-R and TU177-DDP-R cell proliferation, and the expression of MRP1, Bcl-2, and p-PKB was decreased and Bax was increased. Conclusions Down-regulation of BANCR reverses cisplatin resistance of cisplatin-resistant LSCC cell lines.https://www.archivesofmedicalscience.com/Downregulation-of-long-non-coding-RNA-B-Raf-proto-oncogene-activated-non-coding-RNA,98949,0,2.htmllaryngeal squamous cell carcinomab-raf proto-oncogene-activated non-coding rnacisplatinresistance |
| spellingShingle | Weiwei Han Lin Niu Lin Wang Jixiang Liu Huanying Li Downregulation of long non-coding RNA B-Raf proto-oncogene-activated non-coding RNA reverses cisplatin resistance in laryngeal squamous cell carcinoma Archives of Medical Science laryngeal squamous cell carcinoma b-raf proto-oncogene-activated non-coding rna cisplatin resistance |
| title | Downregulation of long non-coding RNA B-Raf proto-oncogene-activated non-coding RNA reverses cisplatin resistance in laryngeal squamous cell carcinoma |
| title_full | Downregulation of long non-coding RNA B-Raf proto-oncogene-activated non-coding RNA reverses cisplatin resistance in laryngeal squamous cell carcinoma |
| title_fullStr | Downregulation of long non-coding RNA B-Raf proto-oncogene-activated non-coding RNA reverses cisplatin resistance in laryngeal squamous cell carcinoma |
| title_full_unstemmed | Downregulation of long non-coding RNA B-Raf proto-oncogene-activated non-coding RNA reverses cisplatin resistance in laryngeal squamous cell carcinoma |
| title_short | Downregulation of long non-coding RNA B-Raf proto-oncogene-activated non-coding RNA reverses cisplatin resistance in laryngeal squamous cell carcinoma |
| title_sort | downregulation of long non coding rna b raf proto oncogene activated non coding rna reverses cisplatin resistance in laryngeal squamous cell carcinoma |
| topic | laryngeal squamous cell carcinoma b-raf proto-oncogene-activated non-coding rna cisplatin resistance |
| url | https://www.archivesofmedicalscience.com/Downregulation-of-long-non-coding-RNA-B-Raf-proto-oncogene-activated-non-coding-RNA,98949,0,2.html |
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