The biological responses of osteoblasts on titanium: Effect of oxygen level and surface roughness
Background/Purpose: Due to the general application of in vitro test, cell culture is generally selected to evaluate the cytocompatibility of devices and materials. The choice of test condition should depend on the probable site and clinical application. The oxygen content of human body could be esti...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Journal of the Formosan Medical Association |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664623000098 |
| _version_ | 1827932969804759040 |
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| author | Chih-Ling Huang Kai-Ting Huang Tzer-Min Lee |
| author_facet | Chih-Ling Huang Kai-Ting Huang Tzer-Min Lee |
| author_sort | Chih-Ling Huang |
| collection | DOAJ |
| description | Background/Purpose: Due to the general application of in vitro test, cell culture is generally selected to evaluate the cytocompatibility of devices and materials. The choice of test condition should depend on the probable site and clinical application. The oxygen content of human body could be estimated around 5%∼12%, and the oxygen level of healing bone fracture range from 0.8%∼3.8%%. However, materials for bone implant are traditionally evaluated under laboratory normoxia condition (21% O2) in vitro. The aim was to study the effect of oxygen level on osteoblast upon high stiffness titanium with different roughness. Methods: After sandblasted and acid-etched (SLA) process, we create titanium surfaces with four different roughness. The differentiation and proliferation of MC3T3-E1 osteoblast cultured on SLA-treated specimens were evaluated in designed chamber with oxygen level of 1%, 5%, 10%, 21%. Results: By scanning electron microscopy, all samples had sub-micro pit inside the micro-holes upon SLA-treated Ti disk surface. The decrease of oxygen level from 21% to 5% promoted osteoblast growth of SLA-treated specimens, but 1% O2 delayed cell proliferation. The surface roughness of specimens influenced osteoblast cell differentiation. The differentiation and proliferation ability of the cells upon SLA-treated specimens is proportional to oxygen level. Conclusion: Our results demonstrated that 5% O2 will easily discriminate osteoblasts responses on different SLA-treated specimens. These results suggest that hypoxia (5% O2) environment is better model for biological evaluation of bone-related materials. |
| first_indexed | 2024-03-13T07:18:02Z |
| format | Article |
| id | doaj.art-614dc93db555470391d0b84000ccd1db |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-03-13T07:18:02Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-614dc93db555470391d0b84000ccd1db2023-06-05T04:12:25ZengElsevierJournal of the Formosan Medical Association0929-66462023-07-011227584592The biological responses of osteoblasts on titanium: Effect of oxygen level and surface roughnessChih-Ling Huang0Kai-Ting Huang1Tzer-Min Lee2Center for Fundamental Science, Kaohsiung Medical University, Kaohsiung, TaiwanDepartment of Biomedical Engineering, National Cheng Kung University, Tainan, TaiwanSchool of Dentistry, National Cheng Kung University, Tainan, Taiwan; Institute of Oral Medicine, National Cheng Kung University, Tainan, Taiwan; Taiwan Instrument Research Institute, National Applied Research Laboratories, Hsinchu, Taiwan; Corresponding author. Institute of Oral Medicine, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan. Fax: +886 6 2359885.Background/Purpose: Due to the general application of in vitro test, cell culture is generally selected to evaluate the cytocompatibility of devices and materials. The choice of test condition should depend on the probable site and clinical application. The oxygen content of human body could be estimated around 5%∼12%, and the oxygen level of healing bone fracture range from 0.8%∼3.8%%. However, materials for bone implant are traditionally evaluated under laboratory normoxia condition (21% O2) in vitro. The aim was to study the effect of oxygen level on osteoblast upon high stiffness titanium with different roughness. Methods: After sandblasted and acid-etched (SLA) process, we create titanium surfaces with four different roughness. The differentiation and proliferation of MC3T3-E1 osteoblast cultured on SLA-treated specimens were evaluated in designed chamber with oxygen level of 1%, 5%, 10%, 21%. Results: By scanning electron microscopy, all samples had sub-micro pit inside the micro-holes upon SLA-treated Ti disk surface. The decrease of oxygen level from 21% to 5% promoted osteoblast growth of SLA-treated specimens, but 1% O2 delayed cell proliferation. The surface roughness of specimens influenced osteoblast cell differentiation. The differentiation and proliferation ability of the cells upon SLA-treated specimens is proportional to oxygen level. Conclusion: Our results demonstrated that 5% O2 will easily discriminate osteoblasts responses on different SLA-treated specimens. These results suggest that hypoxia (5% O2) environment is better model for biological evaluation of bone-related materials.http://www.sciencedirect.com/science/article/pii/S0929664623000098Bone implantHypoxiaOxygen levelSandblasted and acid-etched (SLA)TitaniumOsteoblast |
| spellingShingle | Chih-Ling Huang Kai-Ting Huang Tzer-Min Lee The biological responses of osteoblasts on titanium: Effect of oxygen level and surface roughness Journal of the Formosan Medical Association Bone implant Hypoxia Oxygen level Sandblasted and acid-etched (SLA) Titanium Osteoblast |
| title | The biological responses of osteoblasts on titanium: Effect of oxygen level and surface roughness |
| title_full | The biological responses of osteoblasts on titanium: Effect of oxygen level and surface roughness |
| title_fullStr | The biological responses of osteoblasts on titanium: Effect of oxygen level and surface roughness |
| title_full_unstemmed | The biological responses of osteoblasts on titanium: Effect of oxygen level and surface roughness |
| title_short | The biological responses of osteoblasts on titanium: Effect of oxygen level and surface roughness |
| title_sort | biological responses of osteoblasts on titanium effect of oxygen level and surface roughness |
| topic | Bone implant Hypoxia Oxygen level Sandblasted and acid-etched (SLA) Titanium Osteoblast |
| url | http://www.sciencedirect.com/science/article/pii/S0929664623000098 |
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