Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences
Genetic variation was estimated in ten samples populations of Aedes aegypti from the Brazilian Amazon, by using a 380 bp fragment of the mitochocondrial NADH dehydrogenase subunit 4 (ND4) gene. A total of 123 individuals were analyzed, whereby 13 haplotypes were found. Mean genetic diversity was sli...
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| Format: | Article |
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Sociedade Brasileira de Genética
2009-01-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200032 |
| _version_ | 1819059594017636352 |
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| author | Raimundo Sousa Lima Júnior Vera Margarete Scarpassa |
| author_facet | Raimundo Sousa Lima Júnior Vera Margarete Scarpassa |
| author_sort | Raimundo Sousa Lima Júnior |
| collection | DOAJ |
| description | Genetic variation was estimated in ten samples populations of Aedes aegypti from the Brazilian Amazon, by using a 380 bp fragment of the mitochocondrial NADH dehydrogenase subunit 4 (ND4) gene. A total of 123 individuals were analyzed, whereby 13 haplotypes were found. Mean genetic diversity was slightly high (h = 0.666 ± 0.029; π = 0.0115 ± 0.0010). Two AMOVA analyses indicated that most of the variation (~70%-72%) occurred within populations. The variation found among and between populations within the groups disclosed lower, but even so, highly significant values. F ST values were not significant in most of the comparisons, except for the samples from Pacaraima and Rio Branco. The isolation by distance (IBD) model was not significant (r = 0.2880; p = 0.097) when the samples from Pacaraima and Rio Branco were excluded from the analyses, this indicating that genetic distance is not related to geographic distance. This result may be explained either by passive dispersal patterns (via human migrations and commercial exchange) or be due to the recent expansion of this mosquito in the Brazilian Amazon. Phylogenetic relationship analysis showed two genetically distinct groups (lineages) within the Brazilian Amazon, each sharing haplotypes with populations from West Africa and Asia. |
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| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-21T14:13:34Z |
| publishDate | 2009-01-01 |
| publisher | Sociedade Brasileira de Genética |
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| series | Genetics and Molecular Biology |
| spelling | doaj.art-61522d756d894630b85d11551e769c422022-12-21T19:00:58ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852009-01-01322414422Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequencesRaimundo Sousa Lima JúniorVera Margarete ScarpassaGenetic variation was estimated in ten samples populations of Aedes aegypti from the Brazilian Amazon, by using a 380 bp fragment of the mitochocondrial NADH dehydrogenase subunit 4 (ND4) gene. A total of 123 individuals were analyzed, whereby 13 haplotypes were found. Mean genetic diversity was slightly high (h = 0.666 ± 0.029; π = 0.0115 ± 0.0010). Two AMOVA analyses indicated that most of the variation (~70%-72%) occurred within populations. The variation found among and between populations within the groups disclosed lower, but even so, highly significant values. F ST values were not significant in most of the comparisons, except for the samples from Pacaraima and Rio Branco. The isolation by distance (IBD) model was not significant (r = 0.2880; p = 0.097) when the samples from Pacaraima and Rio Branco were excluded from the analyses, this indicating that genetic distance is not related to geographic distance. This result may be explained either by passive dispersal patterns (via human migrations and commercial exchange) or be due to the recent expansion of this mosquito in the Brazilian Amazon. Phylogenetic relationship analysis showed two genetically distinct groups (lineages) within the Brazilian Amazon, each sharing haplotypes with populations from West Africa and Asia.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200032Aedes aegyptidengue vectorgenetic structuregene flowBrazilian Amazongenetic lineages |
| spellingShingle | Raimundo Sousa Lima Júnior Vera Margarete Scarpassa Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences Genetics and Molecular Biology Aedes aegypti dengue vector genetic structure gene flow Brazilian Amazon genetic lineages |
| title | Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences |
| title_full | Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences |
| title_fullStr | Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences |
| title_full_unstemmed | Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences |
| title_short | Evidence of two lineages of the dengue vector Aedes aegypti in the Brazilian Amazon, based on mitochondrial DNA ND4 gene sequences |
| title_sort | evidence of two lineages of the dengue vector aedes aegypti in the brazilian amazon based on mitochondrial dna nd4 gene sequences |
| topic | Aedes aegypti dengue vector genetic structure gene flow Brazilian Amazon genetic lineages |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200032 |
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