Dutasteride nanoemulsion preparation to inhibit 5‐alpha‐hair follicle reductase enzymes in the hair follicle; an ex vivo study
Abstract Alopecia is a treatable disorder that usually occurs due to high levels of 5‐alpha dihydrotestosterone in hair follicles. To enhance the storage capacity of hair follicles and alleviate the inherent characteristics of dutasteride, 5‐alpha reductase inhibitor, a prolonged‐release nanocarrier...
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| Format: | Article |
| Language: | English |
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Hindawi-IET
2023-02-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/nbt2.12101 |
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| author | Mehri Memar Bashi Aval Elham Hoveizi Reza Mombeiny Mostafa Kazemi Saeedeh Saeedi Shima Tavakol |
| author_facet | Mehri Memar Bashi Aval Elham Hoveizi Reza Mombeiny Mostafa Kazemi Saeedeh Saeedi Shima Tavakol |
| author_sort | Mehri Memar Bashi Aval |
| collection | DOAJ |
| description | Abstract Alopecia is a treatable disorder that usually occurs due to high levels of 5‐alpha dihydrotestosterone in hair follicles. To enhance the storage capacity of hair follicles and alleviate the inherent characteristics of dutasteride, 5‐alpha reductase inhibitor, a prolonged‐release nanocarrier was synthesised, and its influence on rat abdomen's skin was investigated. Results showed the lower ratio of S/Co (higher ethanol concentration) increased the hydrodynamic nanocarriers' particle size due to thermodynamic disturbance and Ostwald ripening. In contrast, an increase in surfactant through a decrease in interfacial tension resulted in smaller nanocarriers of 32.4 nm. Moreover, an increase in viscosity had an inverse correlation with the nanoemulsions' particle size. Nanocarriers containing ethanol showed less entrapment efficacy, perhaps due to the rapid dissolution of dutasteride into ethanol during nanoemulsification, while, based on Stokes' equation, the addition of ethanol resulted in smaller particle size and stability of the system. Skin permeation analysis using Franz diffusion cells showed nanocarriers could pass through the skin and release dutasteride for 6 days. In conclusion, the optimum concentration of ingredients is decisive in guaranteeing the ideal particle size, stability, and skin permeation of nanocarriers. The Present dutasteride nanocarrier would promise a prolonged and sustained‐release drug delivery system for Alopecia therapy. |
| first_indexed | 2024-03-09T07:18:49Z |
| format | Article |
| id | doaj.art-615355de618e4a2eb31e101a70535466 |
| institution | Directory Open Access Journal |
| issn | 1751-8741 1751-875X |
| language | English |
| last_indexed | 2024-03-09T07:18:49Z |
| publishDate | 2023-02-01 |
| publisher | Hindawi-IET |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj.art-615355de618e4a2eb31e101a705354662023-12-03T08:08:20ZengHindawi-IETIET Nanobiotechnology1751-87411751-875X2023-02-01171132110.1049/nbt2.12101Dutasteride nanoemulsion preparation to inhibit 5‐alpha‐hair follicle reductase enzymes in the hair follicle; an ex vivo studyMehri Memar Bashi Aval0Elham Hoveizi1Reza Mombeiny2Mostafa Kazemi3Saeedeh Saeedi4Shima Tavakol5Department of Medical Nanotechnology Faculty of Advanced Sciences and Technology Pharmaceutical Sciences Branch Islamic Azad University, (IAUPS) Tehran IranDepartment of Biology Faculty of Science Shahid Chamran University of Ahvaz Ahvaz IranDepartment of Medical Nanotechnology Faculty of Advanced Technologies in Medicine Iran University of Medical Sciences Tehran IranDepartment of Medical Nanotechnology Faculty of Advanced Technologies in Medicine Iran University of Medical Sciences Tehran IranDepartment of Pharmaceutical Chemistry School of Pharmacy Shahid Beheshti University of Medical Sciences Tehran IranCellular and Molecular Research Center Iran University of Medical Sciences Tehran IranAbstract Alopecia is a treatable disorder that usually occurs due to high levels of 5‐alpha dihydrotestosterone in hair follicles. To enhance the storage capacity of hair follicles and alleviate the inherent characteristics of dutasteride, 5‐alpha reductase inhibitor, a prolonged‐release nanocarrier was synthesised, and its influence on rat abdomen's skin was investigated. Results showed the lower ratio of S/Co (higher ethanol concentration) increased the hydrodynamic nanocarriers' particle size due to thermodynamic disturbance and Ostwald ripening. In contrast, an increase in surfactant through a decrease in interfacial tension resulted in smaller nanocarriers of 32.4 nm. Moreover, an increase in viscosity had an inverse correlation with the nanoemulsions' particle size. Nanocarriers containing ethanol showed less entrapment efficacy, perhaps due to the rapid dissolution of dutasteride into ethanol during nanoemulsification, while, based on Stokes' equation, the addition of ethanol resulted in smaller particle size and stability of the system. Skin permeation analysis using Franz diffusion cells showed nanocarriers could pass through the skin and release dutasteride for 6 days. In conclusion, the optimum concentration of ingredients is decisive in guaranteeing the ideal particle size, stability, and skin permeation of nanocarriers. The Present dutasteride nanocarrier would promise a prolonged and sustained‐release drug delivery system for Alopecia therapy.https://doi.org/10.1049/nbt2.12101Alopeciadutasteridenanocarrierparticle sizesustained‐release |
| spellingShingle | Mehri Memar Bashi Aval Elham Hoveizi Reza Mombeiny Mostafa Kazemi Saeedeh Saeedi Shima Tavakol Dutasteride nanoemulsion preparation to inhibit 5‐alpha‐hair follicle reductase enzymes in the hair follicle; an ex vivo study IET Nanobiotechnology Alopecia dutasteride nanocarrier particle size sustained‐release |
| title | Dutasteride nanoemulsion preparation to inhibit 5‐alpha‐hair follicle reductase enzymes in the hair follicle; an ex vivo study |
| title_full | Dutasteride nanoemulsion preparation to inhibit 5‐alpha‐hair follicle reductase enzymes in the hair follicle; an ex vivo study |
| title_fullStr | Dutasteride nanoemulsion preparation to inhibit 5‐alpha‐hair follicle reductase enzymes in the hair follicle; an ex vivo study |
| title_full_unstemmed | Dutasteride nanoemulsion preparation to inhibit 5‐alpha‐hair follicle reductase enzymes in the hair follicle; an ex vivo study |
| title_short | Dutasteride nanoemulsion preparation to inhibit 5‐alpha‐hair follicle reductase enzymes in the hair follicle; an ex vivo study |
| title_sort | dutasteride nanoemulsion preparation to inhibit 5 alpha hair follicle reductase enzymes in the hair follicle an ex vivo study |
| topic | Alopecia dutasteride nanocarrier particle size sustained‐release |
| url | https://doi.org/10.1049/nbt2.12101 |
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