Biological control of Erwinia mallotivora, the causal agent of papaya dieback disease by indigenous seed-borne endophytic lactic acid bacteria consortium.

Dieback disease caused by Erwinia mallotivora is a major threat to papaya plantation in Malaysia. The current study was conducted to evaluate the potential of endophytic lactic acid bacteria (LAB) isolated from papaya seeds for disease suppression of papaya dieback. Two hundred and thirty isolates w...

Full description

Bibliographic Details
Main Authors: Mariam Dayana Mohd Taha, Mohammad Fahrulazri Mohd Jaini, Noor Baity Saidi, Raha Abdul Rahim, Umi Kalsom Md Shah, Amalia Mohd Hashim
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0224431
_version_ 1818925321135587328
author Mariam Dayana Mohd Taha
Mohammad Fahrulazri Mohd Jaini
Noor Baity Saidi
Raha Abdul Rahim
Umi Kalsom Md Shah
Amalia Mohd Hashim
author_facet Mariam Dayana Mohd Taha
Mohammad Fahrulazri Mohd Jaini
Noor Baity Saidi
Raha Abdul Rahim
Umi Kalsom Md Shah
Amalia Mohd Hashim
author_sort Mariam Dayana Mohd Taha
collection DOAJ
description Dieback disease caused by Erwinia mallotivora is a major threat to papaya plantation in Malaysia. The current study was conducted to evaluate the potential of endophytic lactic acid bacteria (LAB) isolated from papaya seeds for disease suppression of papaya dieback. Two hundred and thirty isolates were screened against E. mallotivora BT-MARDI, and the inhibitory activity of the isolates against the pathogen was ranging from 11.7-23.7 mm inhibition zones. The synergistic experiments revealed that combination of W. cibaria PPKSD19 and Lactococcus lactis subsp. lactis PPSSD39 increased antibacterial activity against the pathogen. The antibacterial activity was partially due to the production of bacteriocin-like inhibitory substances (BLIS). The nursery experiment confirmed that the application of bacterial consortium W. cibaria PPKSD19 and L. lactis subsp. lactis PPSSD39 significantly reduced disease severity to 19% and increased biocontrol efficacy to 69% of infected papaya plants after 18 days of treatment. This study showed that W. cibaria PPKSD19 and L. lactis subsp. lactis PPSSD39 are potential candidate as biocontrol agents against papaya dieback disease.
first_indexed 2024-12-20T02:39:22Z
format Article
id doaj.art-61546be4d8bf4eafb46d4d2725ade1d7
institution Directory Open Access Journal
issn 1932-6203
language English
last_indexed 2024-12-20T02:39:22Z
publishDate 2019-01-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS ONE
spelling doaj.art-61546be4d8bf4eafb46d4d2725ade1d72022-12-21T19:56:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011412e022443110.1371/journal.pone.0224431Biological control of Erwinia mallotivora, the causal agent of papaya dieback disease by indigenous seed-borne endophytic lactic acid bacteria consortium.Mariam Dayana Mohd TahaMohammad Fahrulazri Mohd JainiNoor Baity SaidiRaha Abdul RahimUmi Kalsom Md ShahAmalia Mohd HashimDieback disease caused by Erwinia mallotivora is a major threat to papaya plantation in Malaysia. The current study was conducted to evaluate the potential of endophytic lactic acid bacteria (LAB) isolated from papaya seeds for disease suppression of papaya dieback. Two hundred and thirty isolates were screened against E. mallotivora BT-MARDI, and the inhibitory activity of the isolates against the pathogen was ranging from 11.7-23.7 mm inhibition zones. The synergistic experiments revealed that combination of W. cibaria PPKSD19 and Lactococcus lactis subsp. lactis PPSSD39 increased antibacterial activity against the pathogen. The antibacterial activity was partially due to the production of bacteriocin-like inhibitory substances (BLIS). The nursery experiment confirmed that the application of bacterial consortium W. cibaria PPKSD19 and L. lactis subsp. lactis PPSSD39 significantly reduced disease severity to 19% and increased biocontrol efficacy to 69% of infected papaya plants after 18 days of treatment. This study showed that W. cibaria PPKSD19 and L. lactis subsp. lactis PPSSD39 are potential candidate as biocontrol agents against papaya dieback disease.https://doi.org/10.1371/journal.pone.0224431
spellingShingle Mariam Dayana Mohd Taha
Mohammad Fahrulazri Mohd Jaini
Noor Baity Saidi
Raha Abdul Rahim
Umi Kalsom Md Shah
Amalia Mohd Hashim
Biological control of Erwinia mallotivora, the causal agent of papaya dieback disease by indigenous seed-borne endophytic lactic acid bacteria consortium.
PLoS ONE
title Biological control of Erwinia mallotivora, the causal agent of papaya dieback disease by indigenous seed-borne endophytic lactic acid bacteria consortium.
title_full Biological control of Erwinia mallotivora, the causal agent of papaya dieback disease by indigenous seed-borne endophytic lactic acid bacteria consortium.
title_fullStr Biological control of Erwinia mallotivora, the causal agent of papaya dieback disease by indigenous seed-borne endophytic lactic acid bacteria consortium.
title_full_unstemmed Biological control of Erwinia mallotivora, the causal agent of papaya dieback disease by indigenous seed-borne endophytic lactic acid bacteria consortium.
title_short Biological control of Erwinia mallotivora, the causal agent of papaya dieback disease by indigenous seed-borne endophytic lactic acid bacteria consortium.
title_sort biological control of erwinia mallotivora the causal agent of papaya dieback disease by indigenous seed borne endophytic lactic acid bacteria consortium
url https://doi.org/10.1371/journal.pone.0224431
work_keys_str_mv AT mariamdayanamohdtaha biologicalcontroloferwiniamallotivorathecausalagentofpapayadiebackdiseasebyindigenousseedborneendophyticlacticacidbacteriaconsortium
AT mohammadfahrulazrimohdjaini biologicalcontroloferwiniamallotivorathecausalagentofpapayadiebackdiseasebyindigenousseedborneendophyticlacticacidbacteriaconsortium
AT noorbaitysaidi biologicalcontroloferwiniamallotivorathecausalagentofpapayadiebackdiseasebyindigenousseedborneendophyticlacticacidbacteriaconsortium
AT rahaabdulrahim biologicalcontroloferwiniamallotivorathecausalagentofpapayadiebackdiseasebyindigenousseedborneendophyticlacticacidbacteriaconsortium
AT umikalsommdshah biologicalcontroloferwiniamallotivorathecausalagentofpapayadiebackdiseasebyindigenousseedborneendophyticlacticacidbacteriaconsortium
AT amaliamohdhashim biologicalcontroloferwiniamallotivorathecausalagentofpapayadiebackdiseasebyindigenousseedborneendophyticlacticacidbacteriaconsortium