Insights into Transcriptomic Differences in Ovaries between Lambs and Adult Sheep after Superovulation Treatment
Superovulation technology shows a great potential for shortening breeding time. Using the juvenile superovulation technology, juvenile animals can generate more follicles than adult animals. By sequencing using high-throughput methods, we studied and described differentially expressed (DE) long non-...
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MDPI AG
2023-02-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/4/665 |
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| author | Qingwei Wang Xiaofei Guo Dawei Yao Biao Wang Yupeng Li Jinlong Zhang Xiaosheng Zhang |
| author_facet | Qingwei Wang Xiaofei Guo Dawei Yao Biao Wang Yupeng Li Jinlong Zhang Xiaosheng Zhang |
| author_sort | Qingwei Wang |
| collection | DOAJ |
| description | Superovulation technology shows a great potential for shortening breeding time. Using the juvenile superovulation technology, juvenile animals can generate more follicles than adult animals. By sequencing using high-throughput methods, we studied and described differentially expressed (DE) long non-coding RNA (lncRNAs) and messenger RNAs (mRNAs) in the ovaries of young and adult sheep. Herein, 242 DE lncRNAs and 3150 DE mRNAs were screened. Through GO and KEGG analyses, we obtained genes related to ovarian/follicle development and ovulation in DE mRNAs, including <i>OaFSHR</i>, <i>OaLHCGR</i>, <i>OaLDLR</i>, <i>OaZP3</i>, <i>OaSCARB1</i>, and <i>OaPDGFRA</i>; through lncRNA-mRNA correlation analysis, we found that genes associated with ovarian/follicle development or ovulation include: XR_003585520.1, MSTRG.15652.1, XR_003588840.1, and their paired genes <i>PDGFC</i>, <i>LRP5</i>, and <i>LRP1</i>. We observed a synergistic effect between <i>PDGFR</i> and <i>LRP1</i>. <i>PDGFR</i> may play a leading role compared with <i>LRP1</i>. The induced <i>LHCGR</i> in lambs is higher than in adult sheep, showing more sensitivity to LH. The release of the oocytes was stimulated. Among the three lncRNAs, we found that XR_003588840.1 was significantly different and might perform a regulatory role in ovarian/follicle growth or ovulation. |
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| language | English |
| last_indexed | 2024-03-11T09:15:59Z |
| publishDate | 2023-02-01 |
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| spelling | doaj.art-6154e4f01ccd46e5b0ae31e35a72cf092023-11-16T18:39:50ZengMDPI AGAnimals2076-26152023-02-0113466510.3390/ani13040665Insights into Transcriptomic Differences in Ovaries between Lambs and Adult Sheep after Superovulation TreatmentQingwei Wang0Xiaofei Guo1Dawei Yao2Biao Wang3Yupeng Li4Jinlong Zhang5Xiaosheng Zhang6Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin 300381, ChinaInstitute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin 300381, ChinaInstitute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin 300381, ChinaInstitute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin 300381, ChinaInstitute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin 300381, ChinaInstitute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin 300381, ChinaInstitute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin 300381, ChinaSuperovulation technology shows a great potential for shortening breeding time. Using the juvenile superovulation technology, juvenile animals can generate more follicles than adult animals. By sequencing using high-throughput methods, we studied and described differentially expressed (DE) long non-coding RNA (lncRNAs) and messenger RNAs (mRNAs) in the ovaries of young and adult sheep. Herein, 242 DE lncRNAs and 3150 DE mRNAs were screened. Through GO and KEGG analyses, we obtained genes related to ovarian/follicle development and ovulation in DE mRNAs, including <i>OaFSHR</i>, <i>OaLHCGR</i>, <i>OaLDLR</i>, <i>OaZP3</i>, <i>OaSCARB1</i>, and <i>OaPDGFRA</i>; through lncRNA-mRNA correlation analysis, we found that genes associated with ovarian/follicle development or ovulation include: XR_003585520.1, MSTRG.15652.1, XR_003588840.1, and their paired genes <i>PDGFC</i>, <i>LRP5</i>, and <i>LRP1</i>. We observed a synergistic effect between <i>PDGFR</i> and <i>LRP1</i>. <i>PDGFR</i> may play a leading role compared with <i>LRP1</i>. The induced <i>LHCGR</i> in lambs is higher than in adult sheep, showing more sensitivity to LH. The release of the oocytes was stimulated. Among the three lncRNAs, we found that XR_003588840.1 was significantly different and might perform a regulatory role in ovarian/follicle growth or ovulation.https://www.mdpi.com/2076-2615/13/4/665LncRNAsmRNAsjuvenile superovulationsheep |
| spellingShingle | Qingwei Wang Xiaofei Guo Dawei Yao Biao Wang Yupeng Li Jinlong Zhang Xiaosheng Zhang Insights into Transcriptomic Differences in Ovaries between Lambs and Adult Sheep after Superovulation Treatment Animals LncRNAs mRNAs juvenile superovulation sheep |
| title | Insights into Transcriptomic Differences in Ovaries between Lambs and Adult Sheep after Superovulation Treatment |
| title_full | Insights into Transcriptomic Differences in Ovaries between Lambs and Adult Sheep after Superovulation Treatment |
| title_fullStr | Insights into Transcriptomic Differences in Ovaries between Lambs and Adult Sheep after Superovulation Treatment |
| title_full_unstemmed | Insights into Transcriptomic Differences in Ovaries between Lambs and Adult Sheep after Superovulation Treatment |
| title_short | Insights into Transcriptomic Differences in Ovaries between Lambs and Adult Sheep after Superovulation Treatment |
| title_sort | insights into transcriptomic differences in ovaries between lambs and adult sheep after superovulation treatment |
| topic | LncRNAs mRNAs juvenile superovulation sheep |
| url | https://www.mdpi.com/2076-2615/13/4/665 |
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