Determination of 37 Typical Antibiotics by Liquid Chromatography-Triple Quadrupole Mass Spectrometry
BACKGROUND Currently, the environmental detection of antibiotics is receiving extensive attention, but there is still a lack of methods that can simultaneously analyze seven categories of antibiotics in surface water and groundwater. OBJECTIVES To establish a method that can detect thirty-seven anti...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Science Press, PR China
2022-05-01
|
| Series: | Yankuang ceshi |
| Subjects: | |
| Online Access: | http://www.ykcs.ac.cn/en/article/doi/10.15898/j.cnki.11-2131/td.202111060168 |
| _version_ | 1828065264622632960 |
|---|---|
| author | YING Jiaolong QIN Xiaopeng LANG Hang GUO Jianyi XIONG Ling ZHANG Zhanhao LIU Fei |
| author_facet | YING Jiaolong QIN Xiaopeng LANG Hang GUO Jianyi XIONG Ling ZHANG Zhanhao LIU Fei |
| author_sort | YING Jiaolong |
| collection | DOAJ |
| description | BACKGROUND Currently, the environmental detection of antibiotics is receiving extensive attention, but there is still a lack of methods that can simultaneously analyze seven categories of antibiotics in surface water and groundwater. OBJECTIVES To establish a method that can detect thirty-seven antibiotics (15 sulfonamides, 2 tetracyclines, 3 macrolides, 8 quinolones, 2 chloramphenicols, 2 β-lactams and 5 other kinds) simultaneously. METHODS Solid phase extraction coupled with ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS) was used to quantify the concentration of antibiotics. RESULTS The method had good sensitivity and enrichment effect for the surface water and groundwater, the recoveries ranged from 60% to 130%, the detection limits ranged from 0.6 to 10.6ng/L. The method was applied to the determination of antibiotics in surface water of the Yongding and Chaobai Rivers, and groundwater of the Chaobai River in Beijing. Sulfonamides, quinolones and macrolides were the main antibiotics in the surface water of the Yongding River, and the detection rates were 88.9%, 55.6% and 33.3%, respectively. The highest concentration of floxacin was 111.9ng/L. Sulfonamides, quinolones and chloramphenicols were the main antibiotics in the surface water of the Chaobai River with detection rates of 100%. The highest concentration of tilmicosin was 71.6ng/L. Sulfonamides, quinolones and β-lactams antibiotics were the main antibiotics in the groundwater of the Chaobai River Basin, and the detection rates of were 66.7%, 55.6% and 22.2%, respectively. The highest concentration of sulfadiazine was 69.3ng/L. In both surface water and groundwater, the detection rate and concentration near the sewage treatment plant were significantly higher than those at other points. CONCLUSIONS The method established in this study is simple, rapid and accurate, which can be used for the simultaneous determination of 37 antibiotics in seven categories in surface water and groundwater. It provides antibiotic analysis method support for surface water and groundwater investigation, and provides a scientific basis for rational utilization of water resources and management of new pollutants in water. |
| first_indexed | 2024-04-10T23:12:14Z |
| format | Article |
| id | doaj.art-6155d9a951344e53a0e8aec863d0d5bc |
| institution | Directory Open Access Journal |
| issn | 0254-5357 |
| language | English |
| last_indexed | 2024-04-10T23:12:14Z |
| publishDate | 2022-05-01 |
| publisher | Science Press, PR China |
| record_format | Article |
| series | Yankuang ceshi |
| spelling | doaj.art-6155d9a951344e53a0e8aec863d0d5bc2023-01-13T05:18:57ZengScience Press, PR ChinaYankuang ceshi0254-53572022-05-0141339440310.15898/j.cnki.11-2131/td.202111060168yk202111060168Determination of 37 Typical Antibiotics by Liquid Chromatography-Triple Quadrupole Mass SpectrometryYING Jiaolong0QIN Xiaopeng1LANG Hang2GUO Jianyi3XIONG Ling4ZHANG Zhanhao5LIU Fei6Beijing Key Laboratory of Water Resources and Environmental Engineering, School of Water Resources and Environment, China University of Geosciences (Beijing), Beijing 100083, ChinaTechnical Centre for Soil, Agricultural and Rural Ecology and Environment, Ministry of Ecology and Environment, Beijing 100012, ChinaChina Institute of Water Resources and Hydropower Research, Beijing 100038, ChinaYongding Middle School Affiliated to Capital Normal University, Beijing 102308, ChinaBeijing Key Laboratory of Water Resources and Environmental Engineering, School of Water Resources and Environment, China University of Geosciences (Beijing), Beijing 100083, ChinaBeijing Key Laboratory of Water Resources and Environmental Engineering, School of Water Resources and Environment, China University of Geosciences (Beijing), Beijing 100083, ChinaBeijing Key Laboratory of Water Resources and Environmental Engineering, School of Water Resources and Environment, China University of Geosciences (Beijing), Beijing 100083, ChinaBACKGROUND Currently, the environmental detection of antibiotics is receiving extensive attention, but there is still a lack of methods that can simultaneously analyze seven categories of antibiotics in surface water and groundwater. OBJECTIVES To establish a method that can detect thirty-seven antibiotics (15 sulfonamides, 2 tetracyclines, 3 macrolides, 8 quinolones, 2 chloramphenicols, 2 β-lactams and 5 other kinds) simultaneously. METHODS Solid phase extraction coupled with ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS) was used to quantify the concentration of antibiotics. RESULTS The method had good sensitivity and enrichment effect for the surface water and groundwater, the recoveries ranged from 60% to 130%, the detection limits ranged from 0.6 to 10.6ng/L. The method was applied to the determination of antibiotics in surface water of the Yongding and Chaobai Rivers, and groundwater of the Chaobai River in Beijing. Sulfonamides, quinolones and macrolides were the main antibiotics in the surface water of the Yongding River, and the detection rates were 88.9%, 55.6% and 33.3%, respectively. The highest concentration of floxacin was 111.9ng/L. Sulfonamides, quinolones and chloramphenicols were the main antibiotics in the surface water of the Chaobai River with detection rates of 100%. The highest concentration of tilmicosin was 71.6ng/L. Sulfonamides, quinolones and β-lactams antibiotics were the main antibiotics in the groundwater of the Chaobai River Basin, and the detection rates of were 66.7%, 55.6% and 22.2%, respectively. The highest concentration of sulfadiazine was 69.3ng/L. In both surface water and groundwater, the detection rate and concentration near the sewage treatment plant were significantly higher than those at other points. CONCLUSIONS The method established in this study is simple, rapid and accurate, which can be used for the simultaneous determination of 37 antibiotics in seven categories in surface water and groundwater. It provides antibiotic analysis method support for surface water and groundwater investigation, and provides a scientific basis for rational utilization of water resources and management of new pollutants in water.http://www.ykcs.ac.cn/en/article/doi/10.15898/j.cnki.11-2131/td.202111060168antibioticultra performance liquid chromatography-triple quadrupole-mass spectrometrygroundwatersurface waterbeijing city |
| spellingShingle | YING Jiaolong QIN Xiaopeng LANG Hang GUO Jianyi XIONG Ling ZHANG Zhanhao LIU Fei Determination of 37 Typical Antibiotics by Liquid Chromatography-Triple Quadrupole Mass Spectrometry Yankuang ceshi antibiotic ultra performance liquid chromatography-triple quadrupole-mass spectrometry groundwater surface water beijing city |
| title | Determination of 37 Typical Antibiotics by Liquid Chromatography-Triple Quadrupole Mass Spectrometry |
| title_full | Determination of 37 Typical Antibiotics by Liquid Chromatography-Triple Quadrupole Mass Spectrometry |
| title_fullStr | Determination of 37 Typical Antibiotics by Liquid Chromatography-Triple Quadrupole Mass Spectrometry |
| title_full_unstemmed | Determination of 37 Typical Antibiotics by Liquid Chromatography-Triple Quadrupole Mass Spectrometry |
| title_short | Determination of 37 Typical Antibiotics by Liquid Chromatography-Triple Quadrupole Mass Spectrometry |
| title_sort | determination of 37 typical antibiotics by liquid chromatography triple quadrupole mass spectrometry |
| topic | antibiotic ultra performance liquid chromatography-triple quadrupole-mass spectrometry groundwater surface water beijing city |
| url | http://www.ykcs.ac.cn/en/article/doi/10.15898/j.cnki.11-2131/td.202111060168 |
| work_keys_str_mv | AT yingjiaolong determinationof37typicalantibioticsbyliquidchromatographytriplequadrupolemassspectrometry AT qinxiaopeng determinationof37typicalantibioticsbyliquidchromatographytriplequadrupolemassspectrometry AT langhang determinationof37typicalantibioticsbyliquidchromatographytriplequadrupolemassspectrometry AT guojianyi determinationof37typicalantibioticsbyliquidchromatographytriplequadrupolemassspectrometry AT xiongling determinationof37typicalantibioticsbyliquidchromatographytriplequadrupolemassspectrometry AT zhangzhanhao determinationof37typicalantibioticsbyliquidchromatographytriplequadrupolemassspectrometry AT liufei determinationof37typicalantibioticsbyliquidchromatographytriplequadrupolemassspectrometry |