THE CASE OF TRAUMATIC TRANSMISSION CHILD OF HUMAN IMMUNODEFICIENCY VIRUS
Aim. Analysis of phylogenetic relationships of HIV isolates obtained from a child 8 years old and HIV-positive parents to search for a possible source of infection.Materials and methods. The blood plasma samples of 3 patients (father, mother and child) HIV from Veliky Novgorod were used. Pres...
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| Format: | Article |
| Language: | Russian |
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Journal Infectology
2016-12-01
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| Series: | Журнал инфектологии |
| Subjects: | |
| Online Access: | https://journal.niidi.ru/jofin/article/view/534 |
| _version_ | 1797695217273405440 |
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| author | Yu. V. Ostankova A. V. Semenov M. A. Churina A. P. Rosolovsky E. V. Grebyonkina T. N. Tkachenko T. А. Zhandarmova A. А. Totolian |
| author_facet | Yu. V. Ostankova A. V. Semenov M. A. Churina A. P. Rosolovsky E. V. Grebyonkina T. N. Tkachenko T. А. Zhandarmova A. А. Totolian |
| author_sort | Yu. V. Ostankova |
| collection | DOAJ |
| description | Aim. Analysis of phylogenetic relationships of HIV isolates obtained from a child 8 years old and HIV-positive parents to search for a possible source of infection.Materials and methods. The blood plasma samples of 3 patients (father, mother and child) HIV from Veliky Novgorod were used. Presented in a group of patients were directed to conduct epidemiological investigation cases of HIV-1 infection a child from a dysfunctional family. In the present study we used genotyping by direct sequencing of the site of the polymerase gene (pol) length of 1285 nt., The gene encoding the protease (PR) length of 465 nt. and a portion of the reverse transcriptase (RT) gene length of 820 nt.Results. The study allowed the identification of the HIV virus in clinical samples. HIV-1 subtype A1 (IDU-A) was detected in all cases. Phylogenetic analysis of isolates, where the control samples using HIV-1 isolates obtained previously from Veliky Novgorod, possible to identify grouped in a separate cluster sample from the mother, father and child, which makes it possible to conclude about intrafamily HIV infected child by one of his parents. The nucleotide identity of the samples obtained from the mother and the child, showed a higher percentage of similarity – 98.4%, compared with the identity of the samples between the father and the mother and between father and child (96.2% and 94.2%, respectively). Differences natural polymorphisms in protease and reverse transcriptase genes of the parents and the child are discussed. Conclusion. The analysis of phylogenetic relationships of HIV isolates showed that the source of infection of the child 8 years old, born and living in socially disadvantaged families with HIV-positive parents, is his mother. Way parenteral transmission of infection by random simultaneous trauma in the mother and child.Molecular phylogenetic analysis using routine methods for determining the resistance of HIV to antiretroviral drugs allows to investigate cases of HIV infection, identifying the source of infection. |
| first_indexed | 2024-03-12T03:09:15Z |
| format | Article |
| id | doaj.art-6157a62b687c4a4a962e7e4cf591ce61 |
| institution | Directory Open Access Journal |
| issn | 2072-6732 |
| language | Russian |
| last_indexed | 2024-03-12T03:09:15Z |
| publishDate | 2016-12-01 |
| publisher | Journal Infectology |
| record_format | Article |
| series | Журнал инфектологии |
| spelling | doaj.art-6157a62b687c4a4a962e7e4cf591ce612023-09-03T14:29:57ZrusJournal InfectologyЖурнал инфектологии2072-67322016-12-01849810310.22625/2072-6732-2016-8-4-98-103519THE CASE OF TRAUMATIC TRANSMISSION CHILD OF HUMAN IMMUNODEFICIENCY VIRUSYu. V. Ostankova0A. V. Semenov1M. A. Churina2A. P. Rosolovsky3E. V. Grebyonkina4T. N. Tkachenko5T. А. Zhandarmova6A. А. Totolian7Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии имени ПастераСанкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии имени Пастера; Первый Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова; Северо-Западный государственный медицинский университет имени И.И. МечниковаСанкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии имени ПастераУправление Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека по Новгородской областиУправление Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека по Новгородской областиНовгородский центр по профилактике и борьбе со СПИД и инфекционными заболеваниями «Хелпер»Новгородский центр по профилактике и борьбе со СПИД и инфекционными заболеваниями «Хелпер»Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии имени Пастера; Первый Санкт-Петербургский государственный медицинский университет имени академика И.П. ПавловаAim. Analysis of phylogenetic relationships of HIV isolates obtained from a child 8 years old and HIV-positive parents to search for a possible source of infection.Materials and methods. The blood plasma samples of 3 patients (father, mother and child) HIV from Veliky Novgorod were used. Presented in a group of patients were directed to conduct epidemiological investigation cases of HIV-1 infection a child from a dysfunctional family. In the present study we used genotyping by direct sequencing of the site of the polymerase gene (pol) length of 1285 nt., The gene encoding the protease (PR) length of 465 nt. and a portion of the reverse transcriptase (RT) gene length of 820 nt.Results. The study allowed the identification of the HIV virus in clinical samples. HIV-1 subtype A1 (IDU-A) was detected in all cases. Phylogenetic analysis of isolates, where the control samples using HIV-1 isolates obtained previously from Veliky Novgorod, possible to identify grouped in a separate cluster sample from the mother, father and child, which makes it possible to conclude about intrafamily HIV infected child by one of his parents. The nucleotide identity of the samples obtained from the mother and the child, showed a higher percentage of similarity – 98.4%, compared with the identity of the samples between the father and the mother and between father and child (96.2% and 94.2%, respectively). Differences natural polymorphisms in protease and reverse transcriptase genes of the parents and the child are discussed. Conclusion. The analysis of phylogenetic relationships of HIV isolates showed that the source of infection of the child 8 years old, born and living in socially disadvantaged families with HIV-positive parents, is his mother. Way parenteral transmission of infection by random simultaneous trauma in the mother and child.Molecular phylogenetic analysis using routine methods for determining the resistance of HIV to antiretroviral drugs allows to investigate cases of HIV infection, identifying the source of infection.https://journal.niidi.ru/jofin/article/view/534вичмолекулярная эпидемиологияген polпротеазаобратная транскриптазасубтипмолекулярно-эпидемиологическое расследование |
| spellingShingle | Yu. V. Ostankova A. V. Semenov M. A. Churina A. P. Rosolovsky E. V. Grebyonkina T. N. Tkachenko T. А. Zhandarmova A. А. Totolian THE CASE OF TRAUMATIC TRANSMISSION CHILD OF HUMAN IMMUNODEFICIENCY VIRUS Журнал инфектологии вич молекулярная эпидемиология ген pol протеаза обратная транскриптаза субтип молекулярно-эпидемиологическое расследование |
| title | THE CASE OF TRAUMATIC TRANSMISSION CHILD OF HUMAN IMMUNODEFICIENCY VIRUS |
| title_full | THE CASE OF TRAUMATIC TRANSMISSION CHILD OF HUMAN IMMUNODEFICIENCY VIRUS |
| title_fullStr | THE CASE OF TRAUMATIC TRANSMISSION CHILD OF HUMAN IMMUNODEFICIENCY VIRUS |
| title_full_unstemmed | THE CASE OF TRAUMATIC TRANSMISSION CHILD OF HUMAN IMMUNODEFICIENCY VIRUS |
| title_short | THE CASE OF TRAUMATIC TRANSMISSION CHILD OF HUMAN IMMUNODEFICIENCY VIRUS |
| title_sort | case of traumatic transmission child of human immunodeficiency virus |
| topic | вич молекулярная эпидемиология ген pol протеаза обратная транскриптаза субтип молекулярно-эпидемиологическое расследование |
| url | https://journal.niidi.ru/jofin/article/view/534 |
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