Differentially methylated CpG sites related to fertility in Japanese Black bull spermatozoa: epigenetic biomarker candidates to predict sire conception rate
For semen suppliers, predicting the low fertility of service bull candidates before artificial insemination would help prevent economic loss; however, predicting bull fertility through in vitro assessment of semen is yet to be established. In the present study, we focused on the methylated CpG sites...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2021-01-01
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| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/67/2/67_2020-137/_pdf/-char/en |
| _version_ | 1797615581887725568 |
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| author | Kumiko TAKEDA Eiji KOBAYASHI Kazuko OGATA Akira IMAI Shinya SATO Hiromichi ADACHI Yoichiro HOSHINO Kagetomo NISHINO Masahiro INOUE Masahiro KANEDA Shinya WATANABE |
| author_facet | Kumiko TAKEDA Eiji KOBAYASHI Kazuko OGATA Akira IMAI Shinya SATO Hiromichi ADACHI Yoichiro HOSHINO Kagetomo NISHINO Masahiro INOUE Masahiro KANEDA Shinya WATANABE |
| author_sort | Kumiko TAKEDA |
| collection | DOAJ |
| description | For semen suppliers, predicting the low fertility of service bull candidates before artificial insemination would help prevent economic loss; however, predicting bull fertility through in vitro assessment of semen is yet to be established. In the present study, we focused on the methylated CpG sites of sperm nuclear DNA and examined methylation levels to screen new biomarkers for predicting bull fertility. In frozen-thawed semen samples collected from Japanese Black bulls, for which the sire conception rate (SCR) was recorded, the methylation level of each CpG site was analyzed using human methylation microarray. According to regression analysis, 143 CpG sites related to SCR were significantly differentially methylated. Whole genome bisulfite sequence data were obtained from three semen samples and the differentially methylated regions (DMRs) that included the target CpG sites selected by human methylation microarray were confirmed. Using combined bisulfite restriction analysis, fertility-related methylation changes were detected in 10 DMRs. With the exception of one DMR, the methylation levels of these DMRs were significantly different between groups with high fertility (> 50%) and low fertility (< 40%). From multiple regression analysis of methylation levels and SCR, three DMRs were selected that could effectively predict bull fertility. We suggest that these fertility-related differences in spermatozoal methylation levels could be new epigenetic biomarkers for predicting bull fertility. |
| first_indexed | 2024-03-11T07:28:39Z |
| format | Article |
| id | doaj.art-6157b8329f24477b9b67e799c125a3f1 |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T07:28:39Z |
| publishDate | 2021-01-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-6157b8329f24477b9b67e799c125a3f12023-11-17T07:27:41ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002021-01-016729910710.1262/jrd.2020-137jrdDifferentially methylated CpG sites related to fertility in Japanese Black bull spermatozoa: epigenetic biomarker candidates to predict sire conception rateKumiko TAKEDA0Eiji KOBAYASHI1Kazuko OGATA2Akira IMAI3Shinya SATO4Hiromichi ADACHI5Yoichiro HOSHINO6Kagetomo NISHINO7Masahiro INOUE8Masahiro KANEDA9Shinya WATANABE10Institute of Livestock and Grassland Science, NARO, Ibaraki 305-0901, JapanInstitute of Livestock and Grassland Science, NARO, Ibaraki 305-0901, JapanInstitute of Livestock and Grassland Science, NARO, Ibaraki 305-0901, JapanHiroshima Prefectural Livestock Technology Research Center, Hiroshima 739-0151, JapanHiroshima Prefectural Livestock Technology Research Center, Hiroshima 739-0151, JapanHida Beef Cattle Research, Gifu Prefectural Livestock Research Institute, Gifu 506-0101, JapanKyoto University, Kyoto 622-0203, JapanBeef Cattle Institute, Ibaraki Prefectural Livestock Research Center, Ibaraki 319-2224, JapanTottori Prefectural Livestock Research Institute, Tottori 689-2503, JapanTokyo University of Agriculture and Technology, Tokyo 183-8509, JapanInstitute of Livestock and Grassland Science, NARO, Ibaraki 305-0901, JapanFor semen suppliers, predicting the low fertility of service bull candidates before artificial insemination would help prevent economic loss; however, predicting bull fertility through in vitro assessment of semen is yet to be established. In the present study, we focused on the methylated CpG sites of sperm nuclear DNA and examined methylation levels to screen new biomarkers for predicting bull fertility. In frozen-thawed semen samples collected from Japanese Black bulls, for which the sire conception rate (SCR) was recorded, the methylation level of each CpG site was analyzed using human methylation microarray. According to regression analysis, 143 CpG sites related to SCR were significantly differentially methylated. Whole genome bisulfite sequence data were obtained from three semen samples and the differentially methylated regions (DMRs) that included the target CpG sites selected by human methylation microarray were confirmed. Using combined bisulfite restriction analysis, fertility-related methylation changes were detected in 10 DMRs. With the exception of one DMR, the methylation levels of these DMRs were significantly different between groups with high fertility (> 50%) and low fertility (< 40%). From multiple regression analysis of methylation levels and SCR, three DMRs were selected that could effectively predict bull fertility. We suggest that these fertility-related differences in spermatozoal methylation levels could be new epigenetic biomarkers for predicting bull fertility.https://www.jstage.jst.go.jp/article/jrd/67/2/67_2020-137/_pdf/-char/enbulldna methylationfertilitymicroarrayspermatozoa |
| spellingShingle | Kumiko TAKEDA Eiji KOBAYASHI Kazuko OGATA Akira IMAI Shinya SATO Hiromichi ADACHI Yoichiro HOSHINO Kagetomo NISHINO Masahiro INOUE Masahiro KANEDA Shinya WATANABE Differentially methylated CpG sites related to fertility in Japanese Black bull spermatozoa: epigenetic biomarker candidates to predict sire conception rate The Journal of Reproduction and Development bull dna methylation fertility microarray spermatozoa |
| title | Differentially methylated CpG sites related to fertility in Japanese Black bull spermatozoa: epigenetic biomarker candidates to predict sire conception rate |
| title_full | Differentially methylated CpG sites related to fertility in Japanese Black bull spermatozoa: epigenetic biomarker candidates to predict sire conception rate |
| title_fullStr | Differentially methylated CpG sites related to fertility in Japanese Black bull spermatozoa: epigenetic biomarker candidates to predict sire conception rate |
| title_full_unstemmed | Differentially methylated CpG sites related to fertility in Japanese Black bull spermatozoa: epigenetic biomarker candidates to predict sire conception rate |
| title_short | Differentially methylated CpG sites related to fertility in Japanese Black bull spermatozoa: epigenetic biomarker candidates to predict sire conception rate |
| title_sort | differentially methylated cpg sites related to fertility in japanese black bull spermatozoa epigenetic biomarker candidates to predict sire conception rate |
| topic | bull dna methylation fertility microarray spermatozoa |
| url | https://www.jstage.jst.go.jp/article/jrd/67/2/67_2020-137/_pdf/-char/en |
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