FtsZ Interactions and Biomolecular Condensates as Potential Targets for New Antibiotics
FtsZ is an essential and central protein for cell division in most bacteria. Because of its ability to organize into dynamic polymers at the cell membrane and recruit other protein partners to form a “divisome”, FtsZ is a leading target in the quest for new antibacterial compounds. Strategies to pot...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/10/3/254 |
| _version_ | 1797414863392210944 |
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| author | Silvia Zorrilla Begoña Monterroso Miguel-Ángel Robles-Ramos William Margolin Germán Rivas |
| author_facet | Silvia Zorrilla Begoña Monterroso Miguel-Ángel Robles-Ramos William Margolin Germán Rivas |
| author_sort | Silvia Zorrilla |
| collection | DOAJ |
| description | FtsZ is an essential and central protein for cell division in most bacteria. Because of its ability to organize into dynamic polymers at the cell membrane and recruit other protein partners to form a “divisome”, FtsZ is a leading target in the quest for new antibacterial compounds. Strategies to potentially arrest the essential and tightly regulated cell division process include perturbing FtsZ’s ability to interact with itself and other divisome proteins. Here, we discuss the available methodologies to screen for and characterize those interactions. In addition to assays that measure protein-ligand interactions in solution, we also discuss the use of minimal membrane systems and cell-like compartments to better approximate the native bacterial cell environment and hence provide a more accurate assessment of a candidate compound’s potential in vivo effect. We particularly focus on ways to measure and inhibit under-explored interactions between FtsZ and partner proteins. Finally, we discuss recent evidence that FtsZ forms biomolecular condensates in vitro, and the potential implications of these assemblies in bacterial resistance to antibiotic treatment. |
| first_indexed | 2024-03-09T05:40:04Z |
| format | Article |
| id | doaj.art-615b018d76f74ef4a25db48fc7196cf1 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T05:40:04Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-615b018d76f74ef4a25db48fc7196cf12023-12-03T12:25:29ZengMDPI AGAntibiotics2079-63822021-03-0110325410.3390/antibiotics10030254FtsZ Interactions and Biomolecular Condensates as Potential Targets for New AntibioticsSilvia Zorrilla0Begoña Monterroso1Miguel-Ángel Robles-Ramos2William Margolin3Germán Rivas4Centro de Investigaciones Biológicas Margarita Salas, Consejo Superior de Investigaciones Científicas (CSIC), 28040 Madrid, SpainCentro de Investigaciones Biológicas Margarita Salas, Consejo Superior de Investigaciones Científicas (CSIC), 28040 Madrid, SpainCentro de Investigaciones Biológicas Margarita Salas, Consejo Superior de Investigaciones Científicas (CSIC), 28040 Madrid, SpainDepartment of Microbiology and Molecular Genetics, McGovern Medical School, University of Texas, Houston, TX 77030, USACentro de Investigaciones Biológicas Margarita Salas, Consejo Superior de Investigaciones Científicas (CSIC), 28040 Madrid, SpainFtsZ is an essential and central protein for cell division in most bacteria. Because of its ability to organize into dynamic polymers at the cell membrane and recruit other protein partners to form a “divisome”, FtsZ is a leading target in the quest for new antibacterial compounds. Strategies to potentially arrest the essential and tightly regulated cell division process include perturbing FtsZ’s ability to interact with itself and other divisome proteins. Here, we discuss the available methodologies to screen for and characterize those interactions. In addition to assays that measure protein-ligand interactions in solution, we also discuss the use of minimal membrane systems and cell-like compartments to better approximate the native bacterial cell environment and hence provide a more accurate assessment of a candidate compound’s potential in vivo effect. We particularly focus on ways to measure and inhibit under-explored interactions between FtsZ and partner proteins. Finally, we discuss recent evidence that FtsZ forms biomolecular condensates in vitro, and the potential implications of these assemblies in bacterial resistance to antibiotic treatment.https://www.mdpi.com/2079-6382/10/3/254bacterial cell divisionFtsZ association statesprotein interactionsbiomolecular condensatesmacromolecular crowdingphase separation |
| spellingShingle | Silvia Zorrilla Begoña Monterroso Miguel-Ángel Robles-Ramos William Margolin Germán Rivas FtsZ Interactions and Biomolecular Condensates as Potential Targets for New Antibiotics Antibiotics bacterial cell division FtsZ association states protein interactions biomolecular condensates macromolecular crowding phase separation |
| title | FtsZ Interactions and Biomolecular Condensates as Potential Targets for New Antibiotics |
| title_full | FtsZ Interactions and Biomolecular Condensates as Potential Targets for New Antibiotics |
| title_fullStr | FtsZ Interactions and Biomolecular Condensates as Potential Targets for New Antibiotics |
| title_full_unstemmed | FtsZ Interactions and Biomolecular Condensates as Potential Targets for New Antibiotics |
| title_short | FtsZ Interactions and Biomolecular Condensates as Potential Targets for New Antibiotics |
| title_sort | ftsz interactions and biomolecular condensates as potential targets for new antibiotics |
| topic | bacterial cell division FtsZ association states protein interactions biomolecular condensates macromolecular crowding phase separation |
| url | https://www.mdpi.com/2079-6382/10/3/254 |
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