First TILLING platform in Cucurbita pepo: a new mutant resource for gene function and crop improvement.
Although the availability of genetic and genomic resources for Cucurbita pepo has increased significantly, functional genomic resources are still limited for this crop. In this direction, we have developed a high throughput reverse genetic tool: the first TILLING (Targeting Induced Local Lesions IN...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0112743 |
| _version_ | 1819123292905144320 |
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| author | Nelly Vicente-Dólera Christelle Troadec Manuel Moya Mercedes del Río-Celestino Teresa Pomares-Viciana Abdelhafid Bendahmane Belén Picó Belén Román Pedro Gómez |
| author_facet | Nelly Vicente-Dólera Christelle Troadec Manuel Moya Mercedes del Río-Celestino Teresa Pomares-Viciana Abdelhafid Bendahmane Belén Picó Belén Román Pedro Gómez |
| author_sort | Nelly Vicente-Dólera |
| collection | DOAJ |
| description | Although the availability of genetic and genomic resources for Cucurbita pepo has increased significantly, functional genomic resources are still limited for this crop. In this direction, we have developed a high throughput reverse genetic tool: the first TILLING (Targeting Induced Local Lesions IN Genomes) resource for this species. Additionally, we have used this resource to demonstrate that the previous EMS mutant population we developed has the highest mutation density compared with other cucurbits mutant populations. The overall mutation density in this first C. pepo TILLING platform was estimated to be 1/133 Kb by screening five additional genes. In total, 58 mutations confirmed by sequencing were identified in the five targeted genes, thirteen of which were predicted to have an impact on the function of the protein. The genotype/phenotype correlation was studied in a peroxidase gene, revealing that the phenotype of seedling homozygous for one of the isolated mutant alleles was albino. These results indicate that the TILLING approach in this species was successful at providing new mutations and can address the major challenge of linking sequence information to biological function and also the identification of novel variation for crop breeding. |
| first_indexed | 2024-12-22T07:06:02Z |
| format | Article |
| id | doaj.art-615ecbef50a544549b133f1b9acbfb23 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T07:06:02Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-615ecbef50a544549b133f1b9acbfb232022-12-21T18:34:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11274310.1371/journal.pone.0112743First TILLING platform in Cucurbita pepo: a new mutant resource for gene function and crop improvement.Nelly Vicente-DóleraChristelle TroadecManuel MoyaMercedes del Río-CelestinoTeresa Pomares-VicianaAbdelhafid BendahmaneBelén PicóBelén RománPedro GómezAlthough the availability of genetic and genomic resources for Cucurbita pepo has increased significantly, functional genomic resources are still limited for this crop. In this direction, we have developed a high throughput reverse genetic tool: the first TILLING (Targeting Induced Local Lesions IN Genomes) resource for this species. Additionally, we have used this resource to demonstrate that the previous EMS mutant population we developed has the highest mutation density compared with other cucurbits mutant populations. The overall mutation density in this first C. pepo TILLING platform was estimated to be 1/133 Kb by screening five additional genes. In total, 58 mutations confirmed by sequencing were identified in the five targeted genes, thirteen of which were predicted to have an impact on the function of the protein. The genotype/phenotype correlation was studied in a peroxidase gene, revealing that the phenotype of seedling homozygous for one of the isolated mutant alleles was albino. These results indicate that the TILLING approach in this species was successful at providing new mutations and can address the major challenge of linking sequence information to biological function and also the identification of novel variation for crop breeding.https://doi.org/10.1371/journal.pone.0112743 |
| spellingShingle | Nelly Vicente-Dólera Christelle Troadec Manuel Moya Mercedes del Río-Celestino Teresa Pomares-Viciana Abdelhafid Bendahmane Belén Picó Belén Román Pedro Gómez First TILLING platform in Cucurbita pepo: a new mutant resource for gene function and crop improvement. PLoS ONE |
| title | First TILLING platform in Cucurbita pepo: a new mutant resource for gene function and crop improvement. |
| title_full | First TILLING platform in Cucurbita pepo: a new mutant resource for gene function and crop improvement. |
| title_fullStr | First TILLING platform in Cucurbita pepo: a new mutant resource for gene function and crop improvement. |
| title_full_unstemmed | First TILLING platform in Cucurbita pepo: a new mutant resource for gene function and crop improvement. |
| title_short | First TILLING platform in Cucurbita pepo: a new mutant resource for gene function and crop improvement. |
| title_sort | first tilling platform in cucurbita pepo a new mutant resource for gene function and crop improvement |
| url | https://doi.org/10.1371/journal.pone.0112743 |
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