Efficacy Confirmation Test of Black Cumin (<i>Nigella sativa</i> L.) Seeds Extract Using a High-Fat Diet Mouse Model
To deal with the adverse effects associated with the use of currently available treatments for metabolic disorders, such as type 2 diabetes, there is a need to find an alternative drug compound. In the present study, we investigated the therapeutic potential of black cumin (<i>Nigella sativa&l...
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MDPI AG
2023-03-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/4/501 |
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| author | Khawaja Muhammad Imran Bashir Joo Wan Kim Jong-Kyu Kim Yoon-Seok Chun Jae-Suk Choi Sae-Kwang Ku |
| author_facet | Khawaja Muhammad Imran Bashir Joo Wan Kim Jong-Kyu Kim Yoon-Seok Chun Jae-Suk Choi Sae-Kwang Ku |
| author_sort | Khawaja Muhammad Imran Bashir |
| collection | DOAJ |
| description | To deal with the adverse effects associated with the use of currently available treatments for metabolic disorders, such as type 2 diabetes, there is a need to find an alternative drug compound. In the present study, we investigated the therapeutic potential of black cumin (<i>Nigella sativa</i> L.) seeds extract (BCS extract) for type 2 diabetes using a 45% Kcal-fed obese mouse model. The BCS extract at different doses (400–100 mg/kg) showed a dose-dependent improvement tendency in high-fat diet (HFD)-induced obesity, non-alcoholic fatty liver disease (NAFLD), hyperlipidemia, and diabetic nephropathy compared to the metformin (250 mg/kg). In particular, BCS extract at a dose of 200 mg/kg significantly inhibited the HFD-induced metabolic conditions. The oral administration of BCS extract (200 mg/kg) significantly inhibited the oxidative stress through lipid peroxidation, normalized the activity of sugar metabolism-related enzymes and the expression of genes involved in fat metabolism, and inhibited insulin resistance through glucose and fat metabolism by regulating the 5’-AMP-activated protein kinase (AMPK) expression. Furthermore, BCS extract (200 mg/kg) showed renal damage improvement effects compared to the metformin (250 mg/kg). The results clearly show that BCS aqueous extract at an appropriate concentration could help in the treatment of metabolic disorders, and BCS aqueous extract can be used as a functional food for various diabetic complications, such as obesity, diabetes, and NAFLD. |
| first_indexed | 2024-03-11T04:45:38Z |
| format | Article |
| id | doaj.art-6161c5eb255f44fd96014e1f94af2e7e |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T04:45:38Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-6161c5eb255f44fd96014e1f94af2e7e2023-11-17T20:24:22ZengMDPI AGMetabolites2218-19892023-03-0113450110.3390/metabo13040501Efficacy Confirmation Test of Black Cumin (<i>Nigella sativa</i> L.) Seeds Extract Using a High-Fat Diet Mouse ModelKhawaja Muhammad Imran Bashir0Joo Wan Kim1Jong-Kyu Kim2Yoon-Seok Chun3Jae-Suk Choi4Sae-Kwang Ku5German Engineering Research Center for Life Science Technologies in Medicine and Environment, Busan 46742, Republic of KoreaDepartment of Companion Animal Health, Daegu Haany University, Gyeongsan 38610, Republic of KoreaAriBnC, Ltd., Yongin 16985, Republic of KoreaAriBnC, Ltd., Yongin 16985, Republic of KoreaDepartment of Seafood Science and Technology, The Institute of Marine Industry, Gyeongsang National University, Tongyeong 53064, Republic of KoreaDepartment of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of KoreaTo deal with the adverse effects associated with the use of currently available treatments for metabolic disorders, such as type 2 diabetes, there is a need to find an alternative drug compound. In the present study, we investigated the therapeutic potential of black cumin (<i>Nigella sativa</i> L.) seeds extract (BCS extract) for type 2 diabetes using a 45% Kcal-fed obese mouse model. The BCS extract at different doses (400–100 mg/kg) showed a dose-dependent improvement tendency in high-fat diet (HFD)-induced obesity, non-alcoholic fatty liver disease (NAFLD), hyperlipidemia, and diabetic nephropathy compared to the metformin (250 mg/kg). In particular, BCS extract at a dose of 200 mg/kg significantly inhibited the HFD-induced metabolic conditions. The oral administration of BCS extract (200 mg/kg) significantly inhibited the oxidative stress through lipid peroxidation, normalized the activity of sugar metabolism-related enzymes and the expression of genes involved in fat metabolism, and inhibited insulin resistance through glucose and fat metabolism by regulating the 5’-AMP-activated protein kinase (AMPK) expression. Furthermore, BCS extract (200 mg/kg) showed renal damage improvement effects compared to the metformin (250 mg/kg). The results clearly show that BCS aqueous extract at an appropriate concentration could help in the treatment of metabolic disorders, and BCS aqueous extract can be used as a functional food for various diabetic complications, such as obesity, diabetes, and NAFLD.https://www.mdpi.com/2218-1989/13/4/501black cuminmetabolic disorderNAFLDobese diabetic micetype 2 diabetes |
| spellingShingle | Khawaja Muhammad Imran Bashir Joo Wan Kim Jong-Kyu Kim Yoon-Seok Chun Jae-Suk Choi Sae-Kwang Ku Efficacy Confirmation Test of Black Cumin (<i>Nigella sativa</i> L.) Seeds Extract Using a High-Fat Diet Mouse Model Metabolites black cumin metabolic disorder NAFLD obese diabetic mice type 2 diabetes |
| title | Efficacy Confirmation Test of Black Cumin (<i>Nigella sativa</i> L.) Seeds Extract Using a High-Fat Diet Mouse Model |
| title_full | Efficacy Confirmation Test of Black Cumin (<i>Nigella sativa</i> L.) Seeds Extract Using a High-Fat Diet Mouse Model |
| title_fullStr | Efficacy Confirmation Test of Black Cumin (<i>Nigella sativa</i> L.) Seeds Extract Using a High-Fat Diet Mouse Model |
| title_full_unstemmed | Efficacy Confirmation Test of Black Cumin (<i>Nigella sativa</i> L.) Seeds Extract Using a High-Fat Diet Mouse Model |
| title_short | Efficacy Confirmation Test of Black Cumin (<i>Nigella sativa</i> L.) Seeds Extract Using a High-Fat Diet Mouse Model |
| title_sort | efficacy confirmation test of black cumin i nigella sativa i l seeds extract using a high fat diet mouse model |
| topic | black cumin metabolic disorder NAFLD obese diabetic mice type 2 diabetes |
| url | https://www.mdpi.com/2218-1989/13/4/501 |
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