Label-free Raman imaging for screening of anti-inflammatory function food
Natural bioactive compounds and plant constituents are considered to have a positive anti-inflammatory effect. This study aimed to establish a screening technique for anti-inflammatory function in foods based on label-free Raman imaging. A visible anti-inflammatory analysis method based on coherent...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524001846 |
| _version_ | 1797253907082117120 |
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| author | Qi Zeng Yangyao Peng Xianzhen Zhou Jiaojiao Zhang Yuhang Yang Xinyi Xu Bin Guan Yuntian Zhang Xiaojia Hu Xueli Chen |
| author_facet | Qi Zeng Yangyao Peng Xianzhen Zhou Jiaojiao Zhang Yuhang Yang Xinyi Xu Bin Guan Yuntian Zhang Xiaojia Hu Xueli Chen |
| author_sort | Qi Zeng |
| collection | DOAJ |
| description | Natural bioactive compounds and plant constituents are considered to have a positive anti-inflammatory effect. This study aimed to establish a screening technique for anti-inflammatory function in foods based on label-free Raman imaging. A visible anti-inflammatory analysis method based on coherent anti-Stokes Raman scattering (CARS) was established with an LPS-induced RAW264.7 cell model. Dynamic changes in proteins and lipids were determined at laser pump light wavelengths of 2956 cm−1 and 2856 cm−1, respectively. The method was applied to a plant-based formula (JC) with anti-inflammatory activity. Q-TOF-MS and HPLC analyses revealed the main active constituents of JC as quercetin, kaempferol, l-glutamine, and sodium copper chlorophyllin. In in vitro and in vivo verification experiments, JC showed significant anti-inflammatory activity by regulating the TLR4/NF-κB pathway. In conclusion, this study successfully established a label-free and visible method for screening anti-inflammatory constituents in plant-based food products, which will facilitate the evaluation of functional foods. |
| first_indexed | 2024-04-24T21:41:31Z |
| format | Article |
| id | doaj.art-616265a8338244579750e468d7ea234f |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-04-24T21:41:31Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-616265a8338244579750e468d7ea234f2024-03-21T05:37:30ZengElsevierFood Chemistry: X2590-15752024-06-0122101297Label-free Raman imaging for screening of anti-inflammatory function foodQi Zeng0Yangyao Peng1Xianzhen Zhou2Jiaojiao Zhang3Yuhang Yang4Xinyi Xu5Bin Guan6Yuntian Zhang7Xiaojia Hu8Xueli Chen9Center for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, China; Innovation Center for Advanced Medical Imaging and Intelligent Medicine, Guangzhou Institute of Technology, Xidian University, Guangzhou, Guangdong 510555, ChinaCenter for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, ChinaCenter for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, ChinaCenter for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, ChinaCenter for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, ChinaCenter for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, China; Innovation Center for Advanced Medical Imaging and Intelligent Medicine, Guangzhou Institute of Technology, Xidian University, Guangzhou, Guangdong 510555, ChinaAffiliated Wuxi Fifth Hospital of Jiangnan University, Wuxi Fifth People's Hospital, Wuxi 214000, ChinaShanghai Nature's Sunshine Health Products Co. Ltd, Shanghai 200040, ChinaShanghai Nature's Sunshine Health Products Co. Ltd, Shanghai 200040, China; Corresponding author.Center for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, Xidian University, Xi'an, Shaanxi 710126, China; Innovation Center for Advanced Medical Imaging and Intelligent Medicine, Guangzhou Institute of Technology, Xidian University, Guangzhou, Guangdong 510555, China; Corresponding author at: Center for Biomedical-photonics and Molecular Imaging, Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China.Natural bioactive compounds and plant constituents are considered to have a positive anti-inflammatory effect. This study aimed to establish a screening technique for anti-inflammatory function in foods based on label-free Raman imaging. A visible anti-inflammatory analysis method based on coherent anti-Stokes Raman scattering (CARS) was established with an LPS-induced RAW264.7 cell model. Dynamic changes in proteins and lipids were determined at laser pump light wavelengths of 2956 cm−1 and 2856 cm−1, respectively. The method was applied to a plant-based formula (JC) with anti-inflammatory activity. Q-TOF-MS and HPLC analyses revealed the main active constituents of JC as quercetin, kaempferol, l-glutamine, and sodium copper chlorophyllin. In in vitro and in vivo verification experiments, JC showed significant anti-inflammatory activity by regulating the TLR4/NF-κB pathway. In conclusion, this study successfully established a label-free and visible method for screening anti-inflammatory constituents in plant-based food products, which will facilitate the evaluation of functional foods.http://www.sciencedirect.com/science/article/pii/S2590157524001846Anti-inflammatoryPlant-based ingredientRaman imaging techniqueCoherent anti-stokes Raman scattering |
| spellingShingle | Qi Zeng Yangyao Peng Xianzhen Zhou Jiaojiao Zhang Yuhang Yang Xinyi Xu Bin Guan Yuntian Zhang Xiaojia Hu Xueli Chen Label-free Raman imaging for screening of anti-inflammatory function food Food Chemistry: X Anti-inflammatory Plant-based ingredient Raman imaging technique Coherent anti-stokes Raman scattering |
| title | Label-free Raman imaging for screening of anti-inflammatory function food |
| title_full | Label-free Raman imaging for screening of anti-inflammatory function food |
| title_fullStr | Label-free Raman imaging for screening of anti-inflammatory function food |
| title_full_unstemmed | Label-free Raman imaging for screening of anti-inflammatory function food |
| title_short | Label-free Raman imaging for screening of anti-inflammatory function food |
| title_sort | label free raman imaging for screening of anti inflammatory function food |
| topic | Anti-inflammatory Plant-based ingredient Raman imaging technique Coherent anti-stokes Raman scattering |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524001846 |
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