Isolation and characterization of 15 SSR loci for the endangered European tetraploid species Gladiolus palustris (Iridaceae)
Premise Gladiolus palustris (Iridaceae) is an endangered European perennial tetraploid herb with special conservation interest in the European Union. Microsatellite markers can serve as effective tools for the conservation genetics of this species. Methods and Results We utilized a 454 pyrosequencin...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-05-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/aps3.1245 |
| _version_ | 1818380616151859200 |
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| author | Tamás Malkócs Shyryn Almerekova Judit Bereczki Judit Cservenka Emese Meglécz Gábor Sramkó |
| author_facet | Tamás Malkócs Shyryn Almerekova Judit Bereczki Judit Cservenka Emese Meglécz Gábor Sramkó |
| author_sort | Tamás Malkócs |
| collection | DOAJ |
| description | Premise Gladiolus palustris (Iridaceae) is an endangered European perennial tetraploid herb with special conservation interest in the European Union. Microsatellite markers can serve as effective tools for the conservation genetics of this species. Methods and Results We utilized a 454 pyrosequencing approach to identify simple sequence repeat (SSR) regions in a microsatellite‐enriched library. Of all SSR regions, 46 were screened for specific PCR amplification, and 15 were found to be applicable in the target species. We found 1.62–3.08 alleles per population (effective alleles: 1.58–2.08) that indicated moderate to high genetic diversity values (0.28–0.44) in three pilot populations. Cross‐species amplification was less effective in G. imbricatus and G. tenuis. Conclusions The primers reported here can be used for the population genetic characterization of G. palustris. They will help us to better understand the conservation genetics of this highly endangered species. |
| first_indexed | 2024-12-14T02:21:31Z |
| format | Article |
| id | doaj.art-6162bc9bb20b459d992aa1d264b1a4a9 |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-14T02:21:31Z |
| publishDate | 2019-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-6162bc9bb20b459d992aa1d264b1a4a92022-12-21T23:20:29ZengWileyApplications in Plant Sciences2168-04502019-05-0175n/an/a10.1002/aps3.1245Isolation and characterization of 15 SSR loci for the endangered European tetraploid species Gladiolus palustris (Iridaceae)Tamás Malkócs0Shyryn Almerekova1Judit Bereczki2Judit Cservenka3Emese Meglécz4Gábor Sramkó5Department of Botany University of Debrecen Debrecen HungaryDepartment of Biodiversity and Bioresources Al‐Farabi Kazakh National University Almaty KazakhstanDepartment of Evolutionary Zoology and Human Biology University of Debrecen Debrecen HungaryBalaton Uplands National Park Directorate Csopak HungaryAix Marseille Univ, Avignon Université, CNRS, IRD, IMBE Marseille FranceDepartment of Botany University of Debrecen Debrecen HungaryPremise Gladiolus palustris (Iridaceae) is an endangered European perennial tetraploid herb with special conservation interest in the European Union. Microsatellite markers can serve as effective tools for the conservation genetics of this species. Methods and Results We utilized a 454 pyrosequencing approach to identify simple sequence repeat (SSR) regions in a microsatellite‐enriched library. Of all SSR regions, 46 were screened for specific PCR amplification, and 15 were found to be applicable in the target species. We found 1.62–3.08 alleles per population (effective alleles: 1.58–2.08) that indicated moderate to high genetic diversity values (0.28–0.44) in three pilot populations. Cross‐species amplification was less effective in G. imbricatus and G. tenuis. Conclusions The primers reported here can be used for the population genetic characterization of G. palustris. They will help us to better understand the conservation genetics of this highly endangered species.https://doi.org/10.1002/aps3.1245conservationGladiolus palustrisHabitats Directivemicrosatellitepolyploidpopulation genetics |
| spellingShingle | Tamás Malkócs Shyryn Almerekova Judit Bereczki Judit Cservenka Emese Meglécz Gábor Sramkó Isolation and characterization of 15 SSR loci for the endangered European tetraploid species Gladiolus palustris (Iridaceae) Applications in Plant Sciences conservation Gladiolus palustris Habitats Directive microsatellite polyploid population genetics |
| title | Isolation and characterization of 15 SSR loci for the endangered European tetraploid species Gladiolus palustris (Iridaceae) |
| title_full | Isolation and characterization of 15 SSR loci for the endangered European tetraploid species Gladiolus palustris (Iridaceae) |
| title_fullStr | Isolation and characterization of 15 SSR loci for the endangered European tetraploid species Gladiolus palustris (Iridaceae) |
| title_full_unstemmed | Isolation and characterization of 15 SSR loci for the endangered European tetraploid species Gladiolus palustris (Iridaceae) |
| title_short | Isolation and characterization of 15 SSR loci for the endangered European tetraploid species Gladiolus palustris (Iridaceae) |
| title_sort | isolation and characterization of 15 ssr loci for the endangered european tetraploid species gladiolus palustris iridaceae |
| topic | conservation Gladiolus palustris Habitats Directive microsatellite polyploid population genetics |
| url | https://doi.org/10.1002/aps3.1245 |
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