Comparative genomics and pathogenicity potential of members of the Pseudomonas syringae species complex on Prunus spp
Abstract Background Diseases on Prunus spp. have been associated with a large number of phylogenetically different pathovars and species within the P. syringae species complex. Despite their economic significance, there is a severe lack of genomic information of these pathogens. The high phylogeneti...
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| Format: | Article |
| Language: | English |
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BMC
2019-03-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-019-5555-y |
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| author | Michela Ruinelli Jochen Blom Theo H. M. Smits Joël F. Pothier |
| author_facet | Michela Ruinelli Jochen Blom Theo H. M. Smits Joël F. Pothier |
| author_sort | Michela Ruinelli |
| collection | DOAJ |
| description | Abstract Background Diseases on Prunus spp. have been associated with a large number of phylogenetically different pathovars and species within the P. syringae species complex. Despite their economic significance, there is a severe lack of genomic information of these pathogens. The high phylogenetic diversity observed within strains causing disease on Prunus spp. in nature, raised the question whether other strains or species within the P. syringae species complex were potentially pathogenic on Prunus spp. Results To gain insight into the genomic potential of adaptation and virulence in Prunus spp., a total of twelve de novo whole genome sequences of P. syringae pathovars and species found in association with diseases on cherry (sweet, sour and ornamental-cherry) and peach were sequenced. Strains sequenced in this study covered three phylogroups and four clades. These strains were screened in vitro for pathogenicity on Prunus spp. together with additional genome sequenced strains thus covering nine out of thirteen of the currently defined P. syringae phylogroups. Pathogenicity tests revealed that most of the strains caused symptoms in vitro and no obvious link was found between presence of known virulence factors and the observed pathogenicity pattern based on comparative genomics. Non-pathogenic strains were displaying a two to three times higher generation time when grown in rich medium. Conclusion In this study, the first set of complete genomes of cherry associated P. syringae strains as well as the draft genome of the quarantine peach pathogen P. syringae pv. persicae were generated. The obtained genomic data were matched with phenotypic data in order to determine factors related to pathogenicity to Prunus spp. Results of this study suggest that the inability to cause disease on Prunus spp. in vitro is not the result of host specialization but rather linked to metabolic impairments of individual strains. |
| first_indexed | 2024-12-19T15:41:23Z |
| format | Article |
| id | doaj.art-616595bb000b4f2598e3cd2bc59e56da |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-19T15:41:23Z |
| publishDate | 2019-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-616595bb000b4f2598e3cd2bc59e56da2022-12-21T20:15:28ZengBMCBMC Genomics1471-21642019-03-0120111610.1186/s12864-019-5555-yComparative genomics and pathogenicity potential of members of the Pseudomonas syringae species complex on Prunus sppMichela Ruinelli0Jochen Blom1Theo H. M. Smits2Joël F. Pothier3Environmental Genomics and Systems Biology Research Group, Institute for Natural Resources Sciences, Zurich University of Applied SciencesBioinformatics and Systems Biology, Justus-Liebig-University GiessenEnvironmental Genomics and Systems Biology Research Group, Institute for Natural Resources Sciences, Zurich University of Applied SciencesEnvironmental Genomics and Systems Biology Research Group, Institute for Natural Resources Sciences, Zurich University of Applied SciencesAbstract Background Diseases on Prunus spp. have been associated with a large number of phylogenetically different pathovars and species within the P. syringae species complex. Despite their economic significance, there is a severe lack of genomic information of these pathogens. The high phylogenetic diversity observed within strains causing disease on Prunus spp. in nature, raised the question whether other strains or species within the P. syringae species complex were potentially pathogenic on Prunus spp. Results To gain insight into the genomic potential of adaptation and virulence in Prunus spp., a total of twelve de novo whole genome sequences of P. syringae pathovars and species found in association with diseases on cherry (sweet, sour and ornamental-cherry) and peach were sequenced. Strains sequenced in this study covered three phylogroups and four clades. These strains were screened in vitro for pathogenicity on Prunus spp. together with additional genome sequenced strains thus covering nine out of thirteen of the currently defined P. syringae phylogroups. Pathogenicity tests revealed that most of the strains caused symptoms in vitro and no obvious link was found between presence of known virulence factors and the observed pathogenicity pattern based on comparative genomics. Non-pathogenic strains were displaying a two to three times higher generation time when grown in rich medium. Conclusion In this study, the first set of complete genomes of cherry associated P. syringae strains as well as the draft genome of the quarantine peach pathogen P. syringae pv. persicae were generated. The obtained genomic data were matched with phenotypic data in order to determine factors related to pathogenicity to Prunus spp. Results of this study suggest that the inability to cause disease on Prunus spp. in vitro is not the result of host specialization but rather linked to metabolic impairments of individual strains.http://link.springer.com/article/10.1186/s12864-019-5555-yBacterial cankerIn vitro pathogenicity testType III effectorsPhytotoxinsPhytohormons |
| spellingShingle | Michela Ruinelli Jochen Blom Theo H. M. Smits Joël F. Pothier Comparative genomics and pathogenicity potential of members of the Pseudomonas syringae species complex on Prunus spp BMC Genomics Bacterial canker In vitro pathogenicity test Type III effectors Phytotoxins Phytohormons |
| title | Comparative genomics and pathogenicity potential of members of the Pseudomonas syringae species complex on Prunus spp |
| title_full | Comparative genomics and pathogenicity potential of members of the Pseudomonas syringae species complex on Prunus spp |
| title_fullStr | Comparative genomics and pathogenicity potential of members of the Pseudomonas syringae species complex on Prunus spp |
| title_full_unstemmed | Comparative genomics and pathogenicity potential of members of the Pseudomonas syringae species complex on Prunus spp |
| title_short | Comparative genomics and pathogenicity potential of members of the Pseudomonas syringae species complex on Prunus spp |
| title_sort | comparative genomics and pathogenicity potential of members of the pseudomonas syringae species complex on prunus spp |
| topic | Bacterial canker In vitro pathogenicity test Type III effectors Phytotoxins Phytohormons |
| url | http://link.springer.com/article/10.1186/s12864-019-5555-y |
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