Antimicrobial Efficacy of Ten Commercially Available Herbal Dentifrices against Specific Oral Microflora – In Vitro Study
Aim: To determine and compare the antimicrobial efficacy of ten commercially available herbal dentifrices against specific strains of oral microflora using a standard diffusion method at full strength and 1:1 dilution at 24 h. Materials and Methods: The standard strains of Streptococcus. mutans...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2015-04-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/5829/11127_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PFA2(V_AK)_PF2(PAG).pdf |
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| author | S. Sabiha Shaheen Padma Reddy Hemalatha Srikanth Reddy Dolar Doshi Suhas Kulkarni Manoj Kumar |
| author_facet | S. Sabiha Shaheen Padma Reddy Hemalatha Srikanth Reddy Dolar Doshi Suhas Kulkarni Manoj Kumar |
| author_sort | S. Sabiha Shaheen |
| collection | DOAJ |
| description | Aim: To determine and compare the antimicrobial efficacy of ten
commercially available herbal dentifrices against specific strains of
oral microflora using a standard diffusion method at full strength
and 1:1 dilution at 24 h.
Materials and Methods: The standard strains of Streptococcus.
mutans (ATCC 21293), Streptococcus sangius (MTCC 442),
Actinomyces viscosus (ATCC 3268), Staphylococcus aureus
(ATCC 2592), Streptococcus pyogenes (MTCC 442) and Candida
albicans (ATCC 183) were obtained. Antimicrobial efficacy of the
dentifrices was tested in triplicate, at full strength and 1:1 dilution
with the sterile water using a standard diffusion method for 24 h
at 37ºC. The antimicrobial efficacy was tested by observing the
zones of inhibition in millimeters surrounding disk containing the
dentifrice. Mean standard deviation and standard error of mean
of the inhibitory zones was calculated for each herbal dentifrice.
p<0.05 was considered statistically significant.
Results: Danth Kanthi (DK) was the most effective against all the
microorganisms producing larger zones of inhibition at 24 h (F.S –
40+1.5; 1:1 dilution – 40+2.71). Amar Premium (AP) also produced
larger zones of inhibition against all microorganisms except S.
aureus. Of all the dentifrices, least zones of inhibitions i.e., around
5 mm was observed against S. aureus by Amar Premium (AP) and
Dabur Babool (DB) at 24 h.
Conclusion: Based on the results of the present study, it can be
concluded that all herbal dentifrices exhibited antimicrobial activity
against the selected oral microorganisms, with DK being the most
effective. Hence, it can be inferred that herbal dentifrices can also
be recommended like the conventional formulations. |
| first_indexed | 2024-04-13T02:42:00Z |
| format | Article |
| id | doaj.art-6169bd6a077d48ef8883bd91f6475435 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-13T02:42:00Z |
| publishDate | 2015-04-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-6169bd6a077d48ef8883bd91f64754352022-12-22T03:06:10ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-04-0194ZC42ZC4610.7860/JCDR/2015/11127.5829Antimicrobial Efficacy of Ten Commercially Available Herbal Dentifrices against Specific Oral Microflora – In Vitro StudyS. Sabiha Shaheen0Padma Reddy1Hemalatha2Srikanth Reddy3Dolar Doshi4Suhas Kulkarni5Manoj Kumar6Postgraduate Student, Department of Public Health Dentistry, Panineeya Institute of Dental Sciences & Hospital, Hyderabad, India.Reader, Department of Public Health Dentistry, Panineeya Institute of Dental Sciences & Hospital, Hyderabad, India.Professor & Head, Department of Microbiology, National Institute of Nutrition, ICMR, Hyderabad, India.Reader, Department of Public Health Dentistry, Panineeya Institute of Dental Sciences & Hospital, Hyderabad, India.Reader, Department of Public Health Dentistry, Panineeya Institute of Dental Sciences & Hospital, Hyderabad, India.Professor & Head, Department of Public Health Dentistry, Panineeya Institute of Dental Sciences & Hospital, Hyderabad, India.Scientist, Department of Microbiology and Immunology, National Institute of Nutrition, ICMR, Hyderabad, India.Aim: To determine and compare the antimicrobial efficacy of ten commercially available herbal dentifrices against specific strains of oral microflora using a standard diffusion method at full strength and 1:1 dilution at 24 h. Materials and Methods: The standard strains of Streptococcus. mutans (ATCC 21293), Streptococcus sangius (MTCC 442), Actinomyces viscosus (ATCC 3268), Staphylococcus aureus (ATCC 2592), Streptococcus pyogenes (MTCC 442) and Candida albicans (ATCC 183) were obtained. Antimicrobial efficacy of the dentifrices was tested in triplicate, at full strength and 1:1 dilution with the sterile water using a standard diffusion method for 24 h at 37ºC. The antimicrobial efficacy was tested by observing the zones of inhibition in millimeters surrounding disk containing the dentifrice. Mean standard deviation and standard error of mean of the inhibitory zones was calculated for each herbal dentifrice. p<0.05 was considered statistically significant. Results: Danth Kanthi (DK) was the most effective against all the microorganisms producing larger zones of inhibition at 24 h (F.S – 40+1.5; 1:1 dilution – 40+2.71). Amar Premium (AP) also produced larger zones of inhibition against all microorganisms except S. aureus. Of all the dentifrices, least zones of inhibitions i.e., around 5 mm was observed against S. aureus by Amar Premium (AP) and Dabur Babool (DB) at 24 h. Conclusion: Based on the results of the present study, it can be concluded that all herbal dentifrices exhibited antimicrobial activity against the selected oral microorganisms, with DK being the most effective. Hence, it can be inferred that herbal dentifrices can also be recommended like the conventional formulations.https://jcdr.net/articles/PDF/5829/11127_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PFA2(V_AK)_PF2(PAG).pdfdental cariesmicroorganismsoral hygieneoral diseasesperiodontal diseases |
| spellingShingle | S. Sabiha Shaheen Padma Reddy Hemalatha Srikanth Reddy Dolar Doshi Suhas Kulkarni Manoj Kumar Antimicrobial Efficacy of Ten Commercially Available Herbal Dentifrices against Specific Oral Microflora – In Vitro Study Journal of Clinical and Diagnostic Research dental caries microorganisms oral hygiene oral diseases periodontal diseases |
| title | Antimicrobial Efficacy of Ten Commercially Available Herbal Dentifrices against Specific Oral Microflora – In Vitro Study |
| title_full | Antimicrobial Efficacy of Ten Commercially Available Herbal Dentifrices against Specific Oral Microflora – In Vitro Study |
| title_fullStr | Antimicrobial Efficacy of Ten Commercially Available Herbal Dentifrices against Specific Oral Microflora – In Vitro Study |
| title_full_unstemmed | Antimicrobial Efficacy of Ten Commercially Available Herbal Dentifrices against Specific Oral Microflora – In Vitro Study |
| title_short | Antimicrobial Efficacy of Ten Commercially Available Herbal Dentifrices against Specific Oral Microflora – In Vitro Study |
| title_sort | antimicrobial efficacy of ten commercially available herbal dentifrices against specific oral microflora in vitro study |
| topic | dental caries microorganisms oral hygiene oral diseases periodontal diseases |
| url | https://jcdr.net/articles/PDF/5829/11127_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PFA2(V_AK)_PF2(PAG).pdf |
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