Characterization and Hypoglycemic Activity of a Rhamnan-Type Sulfated Polysaccharide Derivative
Polysaccharide chromium (III) derivatives are gaining increasing attention in improving type 2 diabetes. In this study, the sulfated polysaccharide from Enteromorpha prolifera (SPE) with 4.8 kDa was prepared by specific enzymatic hydrolysis. The obtained SPE was used to prepare a rhamnan-type sulfat...
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MDPI AG
2019-01-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/17/1/21 |
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| author | Jie-Fen Cui Han Ye Yu-Jie Zhu Yin-Ping Li Jing-Feng Wang Peng Wang |
| author_facet | Jie-Fen Cui Han Ye Yu-Jie Zhu Yin-Ping Li Jing-Feng Wang Peng Wang |
| author_sort | Jie-Fen Cui |
| collection | DOAJ |
| description | Polysaccharide chromium (III) derivatives are gaining increasing attention in improving type 2 diabetes. In this study, the sulfated polysaccharide from Enteromorpha prolifera (SPE) with 4.8 kDa was prepared by specific enzymatic hydrolysis. The obtained SPE was used to prepare a rhamnan-type sulfated polysaccharide derivative (SPED). Results indicated that O-H, C=O, and S=O were effectively involved in the chelation of SPED (chromium content 20.26%). Acute (half lethal dose > 2.38 g/kg) and sub-acute toxicity showed that SPED had no damaging effects on mice. Anti-diabetic experiment demonstrated that SPED improved glucose metabolism. Moreover, SPED promoted the PI3K/PKB/GSK-3β signaling pathway by regulating mRNA expression of insulin receptors (IR), insulin receptor substrate 2 (IRS-2), phosphatidylinositol 3 kinase (PI3K), protein kinase B (PKB), and glycogen synthase kinase 3β (GSK-3β). In conclusion, the SPED might represent a novel marine-derived candidate against hyperglycemia, which may undergo further pharmaceutical development as a hypoglycemic agent. |
| first_indexed | 2024-04-11T12:44:12Z |
| format | Article |
| id | doaj.art-616f905053c845598c9d01d0d421ece7 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T12:44:12Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-616f905053c845598c9d01d0d421ece72022-12-22T04:23:25ZengMDPI AGMarine Drugs1660-33972019-01-011712110.3390/md17010021md17010021Characterization and Hypoglycemic Activity of a Rhamnan-Type Sulfated Polysaccharide DerivativeJie-Fen Cui0Han Ye1Yu-Jie Zhu2Yin-Ping Li3Jing-Feng Wang4Peng Wang5College of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaPolysaccharide chromium (III) derivatives are gaining increasing attention in improving type 2 diabetes. In this study, the sulfated polysaccharide from Enteromorpha prolifera (SPE) with 4.8 kDa was prepared by specific enzymatic hydrolysis. The obtained SPE was used to prepare a rhamnan-type sulfated polysaccharide derivative (SPED). Results indicated that O-H, C=O, and S=O were effectively involved in the chelation of SPED (chromium content 20.26%). Acute (half lethal dose > 2.38 g/kg) and sub-acute toxicity showed that SPED had no damaging effects on mice. Anti-diabetic experiment demonstrated that SPED improved glucose metabolism. Moreover, SPED promoted the PI3K/PKB/GSK-3β signaling pathway by regulating mRNA expression of insulin receptors (IR), insulin receptor substrate 2 (IRS-2), phosphatidylinositol 3 kinase (PI3K), protein kinase B (PKB), and glycogen synthase kinase 3β (GSK-3β). In conclusion, the SPED might represent a novel marine-derived candidate against hyperglycemia, which may undergo further pharmaceutical development as a hypoglycemic agent.http://www.mdpi.com/1660-3397/17/1/21sulfated polysaccharide derivativestructure characterizationsafety evaluationtype 2 diabetesmolecular mechanism |
| spellingShingle | Jie-Fen Cui Han Ye Yu-Jie Zhu Yin-Ping Li Jing-Feng Wang Peng Wang Characterization and Hypoglycemic Activity of a Rhamnan-Type Sulfated Polysaccharide Derivative Marine Drugs sulfated polysaccharide derivative structure characterization safety evaluation type 2 diabetes molecular mechanism |
| title | Characterization and Hypoglycemic Activity of a Rhamnan-Type Sulfated Polysaccharide Derivative |
| title_full | Characterization and Hypoglycemic Activity of a Rhamnan-Type Sulfated Polysaccharide Derivative |
| title_fullStr | Characterization and Hypoglycemic Activity of a Rhamnan-Type Sulfated Polysaccharide Derivative |
| title_full_unstemmed | Characterization and Hypoglycemic Activity of a Rhamnan-Type Sulfated Polysaccharide Derivative |
| title_short | Characterization and Hypoglycemic Activity of a Rhamnan-Type Sulfated Polysaccharide Derivative |
| title_sort | characterization and hypoglycemic activity of a rhamnan type sulfated polysaccharide derivative |
| topic | sulfated polysaccharide derivative structure characterization safety evaluation type 2 diabetes molecular mechanism |
| url | http://www.mdpi.com/1660-3397/17/1/21 |
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