Genetic diversity of merozoite surface protein-1 C-terminal 42 kDa of Plasmodium falciparum (PfMSP-142) may be greater than previously known in global isolates
Abstract Background The C-terminal 42 kDa region of merozoite surface protein-1 of Plasmodium falciparum (PfMSP-142) is the target of an immune response. It has been recognised as one of the promising candidate antigens for a blood-stage malaria vaccine. Genetic structure of PfMSP-142 has been consi...
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| Format: | Article |
| Language: | English |
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BMC
2018-08-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-018-3027-x |
| _version_ | 1818443717368872960 |
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| author | Thị Lam Thái Hojong Jun Jinyoung Lee Jung-Mi Kang Hương Giang Lê Khin Lin Kyaw Zin Thant Woon-Mok Sohn Tong-Soo Kim Byoung-Kuk Na |
| author_facet | Thị Lam Thái Hojong Jun Jinyoung Lee Jung-Mi Kang Hương Giang Lê Khin Lin Kyaw Zin Thant Woon-Mok Sohn Tong-Soo Kim Byoung-Kuk Na |
| author_sort | Thị Lam Thái |
| collection | DOAJ |
| description | Abstract Background The C-terminal 42 kDa region of merozoite surface protein-1 of Plasmodium falciparum (PfMSP-142) is the target of an immune response. It has been recognised as one of the promising candidate antigens for a blood-stage malaria vaccine. Genetic structure of PfMSP-142 has been considered to be largely conserved in the P. falciparum population. However, only limited information is currently available. This study aimed to analyse genetic diversity and the effect of natural selection on PfMSP-142 among the Myanmar P. falciparum population and compare them with publicly available PfMSP-142 from global P. falciparum populations. Methods A total of 69 P. falciparum clinical isolates collected from Myanmar malaria patients in Upper Myanmar in 2015 were used. The PfMSP-142 region was amplified by polymerase chain reaction, cloned and sequenced. Genetic structure and natural selection of this region were analysed using MEGA4 and DnaSP programs. Polymorphic nature and natural selection in global PfMSP-142 were also investigated. Results All three allele types (MAD20, K1, and RO33) of PfMSP-142 were identified in Myanmar isolates of P. falciparum. Myanmar PfMSP-142 displayed genetic diversity. Most polymorphisms were scattered in blocks 16 and 17. Polymorphisms observed in Myanmar PfMSP-142 showed a similar pattern to those of global PfMSP-142; however, they were not identical to each other. Genetic diversity of Myanmar PfMSP-142 was relatively lower than that of PfMSP-142 from different geographical regions. Evidence of natural selection and recombination were found. Comparative analysis of genetic polymorphism and natural selection in the global PfMSP-142 population suggested that this region was not tightly conserved in global PfMSP-142 as previously thought and is under the complicated influence of natural selection and recombination. Conclusions Global PfMSP-142 revealed limited, but non-negligible, genetic diversity by allele types and geographical origins. Complicated natural selection and potential recombination might have occurred in global PfMSP-142. Comprehensive monitoring of genetic diversity for global PfMSP-142 would be needed to better understand the polymorphic nature and evolutionary aspect of PfMSP-142 in the global P. falciparum population. More thought would be necessary for designing a vaccine based on PfMSP-142. |
| first_indexed | 2024-12-14T19:04:29Z |
| format | Article |
| id | doaj.art-617114f7b52448ed835948d1e1e9eeb8 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-14T19:04:29Z |
| publishDate | 2018-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-617114f7b52448ed835948d1e1e9eeb82022-12-21T22:50:54ZengBMCParasites & Vectors1756-33052018-08-0111111510.1186/s13071-018-3027-xGenetic diversity of merozoite surface protein-1 C-terminal 42 kDa of Plasmodium falciparum (PfMSP-142) may be greater than previously known in global isolatesThị Lam Thái0Hojong Jun1Jinyoung Lee2Jung-Mi Kang3Hương Giang Lê4Khin Lin5Kyaw Zin Thant6Woon-Mok Sohn7Tong-Soo Kim8Byoung-Kuk Na9Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of MedicineDepartment of Tropical Medicine, and Inha Research Institute for Medical Sciences, Inha University College of MedicineDepartment of Tropical Medicine, and Inha Research Institute for Medical Sciences, Inha University College of MedicineDepartment of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of MedicineDepartment of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of MedicineDepartment of Medical Research Pyin Oo Lwin BranchDepartment of Medical Research, Ministry of Health and SportsDepartment of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of MedicineDepartment of Tropical Medicine, and Inha Research Institute for Medical Sciences, Inha University College of MedicineDepartment of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of MedicineAbstract Background The C-terminal 42 kDa region of merozoite surface protein-1 of Plasmodium falciparum (PfMSP-142) is the target of an immune response. It has been recognised as one of the promising candidate antigens for a blood-stage malaria vaccine. Genetic structure of PfMSP-142 has been considered to be largely conserved in the P. falciparum population. However, only limited information is currently available. This study aimed to analyse genetic diversity and the effect of natural selection on PfMSP-142 among the Myanmar P. falciparum population and compare them with publicly available PfMSP-142 from global P. falciparum populations. Methods A total of 69 P. falciparum clinical isolates collected from Myanmar malaria patients in Upper Myanmar in 2015 were used. The PfMSP-142 region was amplified by polymerase chain reaction, cloned and sequenced. Genetic structure and natural selection of this region were analysed using MEGA4 and DnaSP programs. Polymorphic nature and natural selection in global PfMSP-142 were also investigated. Results All three allele types (MAD20, K1, and RO33) of PfMSP-142 were identified in Myanmar isolates of P. falciparum. Myanmar PfMSP-142 displayed genetic diversity. Most polymorphisms were scattered in blocks 16 and 17. Polymorphisms observed in Myanmar PfMSP-142 showed a similar pattern to those of global PfMSP-142; however, they were not identical to each other. Genetic diversity of Myanmar PfMSP-142 was relatively lower than that of PfMSP-142 from different geographical regions. Evidence of natural selection and recombination were found. Comparative analysis of genetic polymorphism and natural selection in the global PfMSP-142 population suggested that this region was not tightly conserved in global PfMSP-142 as previously thought and is under the complicated influence of natural selection and recombination. Conclusions Global PfMSP-142 revealed limited, but non-negligible, genetic diversity by allele types and geographical origins. Complicated natural selection and potential recombination might have occurred in global PfMSP-142. Comprehensive monitoring of genetic diversity for global PfMSP-142 would be needed to better understand the polymorphic nature and evolutionary aspect of PfMSP-142 in the global P. falciparum population. More thought would be necessary for designing a vaccine based on PfMSP-142.http://link.springer.com/article/10.1186/s13071-018-3027-xPlasmodium falciparumPfMSP-142MyanmarPolymorphismNatural selection |
| spellingShingle | Thị Lam Thái Hojong Jun Jinyoung Lee Jung-Mi Kang Hương Giang Lê Khin Lin Kyaw Zin Thant Woon-Mok Sohn Tong-Soo Kim Byoung-Kuk Na Genetic diversity of merozoite surface protein-1 C-terminal 42 kDa of Plasmodium falciparum (PfMSP-142) may be greater than previously known in global isolates Parasites & Vectors Plasmodium falciparum PfMSP-142 Myanmar Polymorphism Natural selection |
| title | Genetic diversity of merozoite surface protein-1 C-terminal 42 kDa of Plasmodium falciparum (PfMSP-142) may be greater than previously known in global isolates |
| title_full | Genetic diversity of merozoite surface protein-1 C-terminal 42 kDa of Plasmodium falciparum (PfMSP-142) may be greater than previously known in global isolates |
| title_fullStr | Genetic diversity of merozoite surface protein-1 C-terminal 42 kDa of Plasmodium falciparum (PfMSP-142) may be greater than previously known in global isolates |
| title_full_unstemmed | Genetic diversity of merozoite surface protein-1 C-terminal 42 kDa of Plasmodium falciparum (PfMSP-142) may be greater than previously known in global isolates |
| title_short | Genetic diversity of merozoite surface protein-1 C-terminal 42 kDa of Plasmodium falciparum (PfMSP-142) may be greater than previously known in global isolates |
| title_sort | genetic diversity of merozoite surface protein 1 c terminal 42 kda of plasmodium falciparum pfmsp 142 may be greater than previously known in global isolates |
| topic | Plasmodium falciparum PfMSP-142 Myanmar Polymorphism Natural selection |
| url | http://link.springer.com/article/10.1186/s13071-018-3027-x |
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