Phenotypic detection of multi-drug and extensively drug resistant Mycobacterium tuberculosis among tuberculosis patients in Kaduna State, North West Nigeria
Background: Tuberculosis (TB) is one of the leading infectious diseases that cause death worldwide; the emergence of drug resistant mycobacterium tuberculosis is of great concern for TB control programme in Nigeria. Aim and objective: To detect multi-drug and extensively drug resistant mycobacterium...
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| Format: | Article |
| Language: | English |
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Zagazig University, Faculty of Medicine
2024-02-01
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| Series: | Microbes and Infectious Diseases |
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| Online Access: | https://mid.journals.ekb.eg/article_307209_fbd9e49ac09b52ec0d090926bed5a64d.pdf |
| _version_ | 1797317253364973568 |
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| author | Kasimu Mamuda Aliyu Sani Steven Olonitola Opaluwa Saraja |
| author_facet | Kasimu Mamuda Aliyu Sani Steven Olonitola Opaluwa Saraja |
| author_sort | Kasimu Mamuda |
| collection | DOAJ |
| description | Background: Tuberculosis (TB) is one of the leading infectious diseases that cause death worldwide; the emergence of drug resistant mycobacterium tuberculosis is of great concern for TB control programme in Nigeria. Aim and objective: To detect multi-drug and extensively drug resistant mycobacterium tuberculosis among tuberculosis patients in Kaduna State North west Nigeria. Study design: This was a cross sectional study among tuberculosis patients attending directly observed treatment short course (DOTS) clinics in the three (3) senatorial districts of Kaduna State, Nigeria. Materials and methods: A total of 360 presumptive drug resistant tuberculosis patients’ samples were collected and decontaminated using modified Petroff method (NaOH-Na-citrate-NALC) from August 2018 to December 2020. Drug susceptibility testing was performed on first- and second-line anti-tuberculosis drugs for the 305 confirmed MTBC isolates using Lowenstein Jensen (LJ) proportion method. Results: A total of 67(21.8%) isolates were pan-susceptible, 176(57.5%) isolates were MDR-TB, rifampicin and isoniazid mono-resistant were 46(15.1%) and 17(5.6%) respectively. For the second line drugs, a total of 271(80.22%) isolates were found to be pan-susceptible and 35(19.88%) isolates were pre-extensively drug resistant TB. Conclusions: These findings established higher prevalence of MDR-TB and pre-extensively resistant TB in Kaduna state. |
| first_indexed | 2024-03-08T03:32:15Z |
| format | Article |
| id | doaj.art-617118565dfb4205b3bd24ba66597c89 |
| institution | Directory Open Access Journal |
| issn | 2682-4132 2682-4140 |
| language | English |
| last_indexed | 2024-03-08T03:32:15Z |
| publishDate | 2024-02-01 |
| publisher | Zagazig University, Faculty of Medicine |
| record_format | Article |
| series | Microbes and Infectious Diseases |
| spelling | doaj.art-617118565dfb4205b3bd24ba66597c892024-02-10T21:57:30ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402024-02-015114815410.21608/mid.2023.219742.1552307209Phenotypic detection of multi-drug and extensively drug resistant Mycobacterium tuberculosis among tuberculosis patients in Kaduna State, North West NigeriaKasimu Mamuda0Aliyu Sani1Steven Olonitola2Opaluwa Saraja3Department of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, NigeriaDepartment of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, NigeriaDepartment of Microbiology, Faculty of Life Sciences, Ahmadu Bello University, Zaria, NigeriaDepartment of Microbiology, Ahmadu Bello University Teaching Hospital, Zaria, NigeriaBackground: Tuberculosis (TB) is one of the leading infectious diseases that cause death worldwide; the emergence of drug resistant mycobacterium tuberculosis is of great concern for TB control programme in Nigeria. Aim and objective: To detect multi-drug and extensively drug resistant mycobacterium tuberculosis among tuberculosis patients in Kaduna State North west Nigeria. Study design: This was a cross sectional study among tuberculosis patients attending directly observed treatment short course (DOTS) clinics in the three (3) senatorial districts of Kaduna State, Nigeria. Materials and methods: A total of 360 presumptive drug resistant tuberculosis patients’ samples were collected and decontaminated using modified Petroff method (NaOH-Na-citrate-NALC) from August 2018 to December 2020. Drug susceptibility testing was performed on first- and second-line anti-tuberculosis drugs for the 305 confirmed MTBC isolates using Lowenstein Jensen (LJ) proportion method. Results: A total of 67(21.8%) isolates were pan-susceptible, 176(57.5%) isolates were MDR-TB, rifampicin and isoniazid mono-resistant were 46(15.1%) and 17(5.6%) respectively. For the second line drugs, a total of 271(80.22%) isolates were found to be pan-susceptible and 35(19.88%) isolates were pre-extensively drug resistant TB. Conclusions: These findings established higher prevalence of MDR-TB and pre-extensively resistant TB in Kaduna state.https://mid.journals.ekb.eg/article_307209_fbd9e49ac09b52ec0d090926bed5a64d.pdftuberculosismdr-tbmtband ntm |
| spellingShingle | Kasimu Mamuda Aliyu Sani Steven Olonitola Opaluwa Saraja Phenotypic detection of multi-drug and extensively drug resistant Mycobacterium tuberculosis among tuberculosis patients in Kaduna State, North West Nigeria Microbes and Infectious Diseases tuberculosis mdr-tb mtb and ntm |
| title | Phenotypic detection of multi-drug and extensively drug resistant Mycobacterium tuberculosis among tuberculosis patients in Kaduna State, North West Nigeria |
| title_full | Phenotypic detection of multi-drug and extensively drug resistant Mycobacterium tuberculosis among tuberculosis patients in Kaduna State, North West Nigeria |
| title_fullStr | Phenotypic detection of multi-drug and extensively drug resistant Mycobacterium tuberculosis among tuberculosis patients in Kaduna State, North West Nigeria |
| title_full_unstemmed | Phenotypic detection of multi-drug and extensively drug resistant Mycobacterium tuberculosis among tuberculosis patients in Kaduna State, North West Nigeria |
| title_short | Phenotypic detection of multi-drug and extensively drug resistant Mycobacterium tuberculosis among tuberculosis patients in Kaduna State, North West Nigeria |
| title_sort | phenotypic detection of multi drug and extensively drug resistant mycobacterium tuberculosis among tuberculosis patients in kaduna state north west nigeria |
| topic | tuberculosis mdr-tb mtb and ntm |
| url | https://mid.journals.ekb.eg/article_307209_fbd9e49ac09b52ec0d090926bed5a64d.pdf |
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