Immunomodulatory effects of L. helveticus WHH2580 fermented milk on an immunosuppressed murine model
Several bacterial strains from traditional fermented foods were screened on a large scale to identify novel candidate probiotics that can be used as starters. Two in vitro cellular models were used to identify strains with immunomodulatory activity. Three strains with pro-inflammatory activity were...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622004236 |
| _version_ | 1811178930202214400 |
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| author | Yanjun Li Su Chen Lie Chen Cailing Chen Xueliang Ren Zhiyao Zheng Luwei Weng Hongjuan Ge Jian Wang Guanchen Liu Xingqian Ye |
| author_facet | Yanjun Li Su Chen Lie Chen Cailing Chen Xueliang Ren Zhiyao Zheng Luwei Weng Hongjuan Ge Jian Wang Guanchen Liu Xingqian Ye |
| author_sort | Yanjun Li |
| collection | DOAJ |
| description | Several bacterial strains from traditional fermented foods were screened on a large scale to identify novel candidate probiotics that can be used as starters. Two in vitro cellular models were used to identify strains with immunomodulatory activity. Three strains with pro-inflammatory activity were selected, and their recovery effect was tested in vivo in an immunosuppressed murine model. Only Lactobacillus helveticus WHH2580 enhanced natural killer cell activity and splenic lymphocyte proliferation. Furthermore, milk fermented using the strain WHH2580 exerted an immunomodulatory effect with enhanced resistance to gastrointestinal conditions, including strong adhesion to HT-29 cells. Whole genome sequencing revealed the presence of a circular chromosome and two circular plasmids; no antibiotic resistance or putative virulence genes were discovered. These findings indicate the safety and positive role of L. helveticus WHH2580 in maintaining host health in mice and suggest that milk fermented with this strain can deliver this strain and thus promote immunity. |
| first_indexed | 2024-04-11T06:26:16Z |
| format | Article |
| id | doaj.art-6178aaa9989d4eb1a2fcbf997b2baa86 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-11T06:26:16Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-6178aaa9989d4eb1a2fcbf997b2baa862022-12-22T04:40:21ZengElsevierJournal of Functional Foods1756-46462022-12-0199105353Immunomodulatory effects of L. helveticus WHH2580 fermented milk on an immunosuppressed murine modelYanjun Li0Su Chen1Lie Chen2Cailing Chen3Xueliang Ren4Zhiyao Zheng5Luwei Weng6Hongjuan Ge7Jian Wang8Guanchen Liu9Xingqian Ye10College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Food and Biological Engineering of Zhejiang Province, Research and Development Department, Hangzhou Wahaha Technology Co. Ltd., Hangzhou Wahaha Group Co. Ltd., Hangzhou 310018, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Research and Development Department, Hangzhou Wahaha Technology Co. Ltd., Hangzhou Wahaha Group Co. Ltd., Hangzhou 310018, China; Corresponding author at: Street 14, Hangzhou Wahaha Technology Co. Ltd., Xiasha ETDZ, Hangzhou, Zhejiang 310058, China.Key Laboratory of Food and Biological Engineering of Zhejiang Province, Research and Development Department, Hangzhou Wahaha Technology Co. Ltd., Hangzhou Wahaha Group Co. Ltd., Hangzhou 310018, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Research and Development Department, Hangzhou Wahaha Technology Co. Ltd., Hangzhou Wahaha Group Co. Ltd., Hangzhou 310018, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Research and Development Department, Hangzhou Wahaha Technology Co. Ltd., Hangzhou Wahaha Group Co. Ltd., Hangzhou 310018, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Research and Development Department, Hangzhou Wahaha Technology Co. Ltd., Hangzhou Wahaha Group Co. Ltd., Hangzhou 310018, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Research and Development Department, Hangzhou Wahaha Technology Co. Ltd., Hangzhou Wahaha Group Co. Ltd., Hangzhou 310018, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Research and Development Department, Hangzhou Wahaha Technology Co. Ltd., Hangzhou Wahaha Group Co. Ltd., Hangzhou 310018, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Research and Development Department, Hangzhou Wahaha Technology Co. Ltd., Hangzhou Wahaha Group Co. Ltd., Hangzhou 310018, ChinaKey Laboratory of Food and Biological Engineering of Zhejiang Province, Research and Development Department, Hangzhou Wahaha Technology Co. Ltd., Hangzhou Wahaha Group Co. Ltd., Hangzhou 310018, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaSeveral bacterial strains from traditional fermented foods were screened on a large scale to identify novel candidate probiotics that can be used as starters. Two in vitro cellular models were used to identify strains with immunomodulatory activity. Three strains with pro-inflammatory activity were selected, and their recovery effect was tested in vivo in an immunosuppressed murine model. Only Lactobacillus helveticus WHH2580 enhanced natural killer cell activity and splenic lymphocyte proliferation. Furthermore, milk fermented using the strain WHH2580 exerted an immunomodulatory effect with enhanced resistance to gastrointestinal conditions, including strong adhesion to HT-29 cells. Whole genome sequencing revealed the presence of a circular chromosome and two circular plasmids; no antibiotic resistance or putative virulence genes were discovered. These findings indicate the safety and positive role of L. helveticus WHH2580 in maintaining host health in mice and suggest that milk fermented with this strain can deliver this strain and thus promote immunity.http://www.sciencedirect.com/science/article/pii/S1756464622004236ImmunomodulatoryLactobacillus helveticusPro-inflammatoryImmunosuppressed murine modelGenome sequence |
| spellingShingle | Yanjun Li Su Chen Lie Chen Cailing Chen Xueliang Ren Zhiyao Zheng Luwei Weng Hongjuan Ge Jian Wang Guanchen Liu Xingqian Ye Immunomodulatory effects of L. helveticus WHH2580 fermented milk on an immunosuppressed murine model Journal of Functional Foods Immunomodulatory Lactobacillus helveticus Pro-inflammatory Immunosuppressed murine model Genome sequence |
| title | Immunomodulatory effects of L. helveticus WHH2580 fermented milk on an immunosuppressed murine model |
| title_full | Immunomodulatory effects of L. helveticus WHH2580 fermented milk on an immunosuppressed murine model |
| title_fullStr | Immunomodulatory effects of L. helveticus WHH2580 fermented milk on an immunosuppressed murine model |
| title_full_unstemmed | Immunomodulatory effects of L. helveticus WHH2580 fermented milk on an immunosuppressed murine model |
| title_short | Immunomodulatory effects of L. helveticus WHH2580 fermented milk on an immunosuppressed murine model |
| title_sort | immunomodulatory effects of l helveticus whh2580 fermented milk on an immunosuppressed murine model |
| topic | Immunomodulatory Lactobacillus helveticus Pro-inflammatory Immunosuppressed murine model Genome sequence |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622004236 |
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