Caffeoylquinic acid enhances proliferation of oligodendrocyte precursor cells
This report evaluates the protective effect of caffeoylquinic acid (CA) injury to oligodendrocyte precursor cells (OPCs) by promoting the formation of oligodendrocytes. Neonatal rat brain was used to isolate primary OPCs and non-lethal CoCl2 was used to induce hypoxic stress to inhibit the different...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2017-10-01
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| Series: | Translational Neuroscience |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/tnsci-2017-0017 |
| _version_ | 1819016217586827264 |
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| author | Yanqin Ying Shaohua Chen Jing Tang Nan Li |
| author_facet | Yanqin Ying Shaohua Chen Jing Tang Nan Li |
| author_sort | Yanqin Ying |
| collection | DOAJ |
| description | This report evaluates the protective effect of caffeoylquinic acid (CA) injury to oligodendrocyte precursor cells (OPCs) by promoting the formation of oligodendrocytes. Neonatal rat brain was used to isolate primary OPCs and non-lethal CoCl2 was used to induce hypoxic stress to inhibit the differentiation of OPCs. Differentiation of OPCs was estimated by survival assay and the expressions of myelin-basic-protein (MBP). Moreover, the effect of CA on the Akt signanling pathway was also estimated in the presence and absence of LY294002 (PI3K/Akt inhibitor) and adrenomedullin (AM) receptor antagonist (AM22-52) by using western blot assay. It was observed that CA enhances the differentiation OPCs in CoCl2 induced hypoxic stress condition. However treatment with CA in presence of LY294002 and AM22-52 was not able to enhance the differentiation of OPCs. Moreover treatment with CA significantly enhances the phosphorylation of Akt and presence of LY294002 and AM22-52 inhibits it. This report concludes that CA effectively attenuates the injury of white matter (OPCs) by enhancing the differentiation of OPCs. It enhances the formation of oligodendrocytes by activating AM receptor and thereby accelerates the regeneration of neuron in pathological condition. |
| first_indexed | 2024-12-21T02:44:07Z |
| format | Article |
| id | doaj.art-6178bda6b1264b90a37dfda952ec9ed6 |
| institution | Directory Open Access Journal |
| issn | 2081-6936 |
| language | English |
| last_indexed | 2024-12-21T02:44:07Z |
| publishDate | 2017-10-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Translational Neuroscience |
| spelling | doaj.art-6178bda6b1264b90a37dfda952ec9ed62022-12-21T19:18:36ZengDe GruyterTranslational Neuroscience2081-69362017-10-018111111610.1515/tnsci-2017-0017tnsci-2017-0017Caffeoylquinic acid enhances proliferation of oligodendrocyte precursor cellsYanqin Ying0Shaohua Chen1Jing Tang2Nan Li3Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030ChinaDepartment of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030ChinaDepartment of Pediatrics, Jinzhou Maternal and Children Healthy Care Hospital, Jinzhou, 434020ChinaDepartment of intensive care unit, The first hospital of Jilin University, Changchun, 130021ChinaThis report evaluates the protective effect of caffeoylquinic acid (CA) injury to oligodendrocyte precursor cells (OPCs) by promoting the formation of oligodendrocytes. Neonatal rat brain was used to isolate primary OPCs and non-lethal CoCl2 was used to induce hypoxic stress to inhibit the differentiation of OPCs. Differentiation of OPCs was estimated by survival assay and the expressions of myelin-basic-protein (MBP). Moreover, the effect of CA on the Akt signanling pathway was also estimated in the presence and absence of LY294002 (PI3K/Akt inhibitor) and adrenomedullin (AM) receptor antagonist (AM22-52) by using western blot assay. It was observed that CA enhances the differentiation OPCs in CoCl2 induced hypoxic stress condition. However treatment with CA in presence of LY294002 and AM22-52 was not able to enhance the differentiation of OPCs. Moreover treatment with CA significantly enhances the phosphorylation of Akt and presence of LY294002 and AM22-52 inhibits it. This report concludes that CA effectively attenuates the injury of white matter (OPCs) by enhancing the differentiation of OPCs. It enhances the formation of oligodendrocytes by activating AM receptor and thereby accelerates the regeneration of neuron in pathological condition.https://doi.org/10.1515/tnsci-2017-0017caffeoylquinic acidoligodendrocyte precursor cellsmyelin-basic-proteinadrenomedullin |
| spellingShingle | Yanqin Ying Shaohua Chen Jing Tang Nan Li Caffeoylquinic acid enhances proliferation of oligodendrocyte precursor cells Translational Neuroscience caffeoylquinic acid oligodendrocyte precursor cells myelin-basic-protein adrenomedullin |
| title | Caffeoylquinic acid enhances proliferation of oligodendrocyte precursor cells |
| title_full | Caffeoylquinic acid enhances proliferation of oligodendrocyte precursor cells |
| title_fullStr | Caffeoylquinic acid enhances proliferation of oligodendrocyte precursor cells |
| title_full_unstemmed | Caffeoylquinic acid enhances proliferation of oligodendrocyte precursor cells |
| title_short | Caffeoylquinic acid enhances proliferation of oligodendrocyte precursor cells |
| title_sort | caffeoylquinic acid enhances proliferation of oligodendrocyte precursor cells |
| topic | caffeoylquinic acid oligodendrocyte precursor cells myelin-basic-protein adrenomedullin |
| url | https://doi.org/10.1515/tnsci-2017-0017 |
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