Diversity of Mobile Genetic Elements in the Mitogenomes of Closely Related Fusarium culmorum and F. graminearum sensu stricto Strains and Its Implication for Diagnostic Purposes
Much of the mitogenome variation observed in fungal lineages seems driven by mobile genetic elements (MGEs), which have invaded their genomes throughout evolution. The variation in the distribution and nucleotide diversity of these elements appears to be the main distinction between different fungal...
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| Format: | Article |
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01002/full |
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| author | Tomasz Kulik Balazs Brankovics Anne D. van Diepeningen Katarzyna Bilska Maciej Żelechowski Kamil Myszczyński Kamil Myszczyński Tomasz Molcan Alexander Stakheev Sebastian Stenglein Sebastian Stenglein Marco Beyer Matias Pasquali Jakub Sawicki Joanna Wyrȩbek Anna Baturo-Cieśniewska |
| author_facet | Tomasz Kulik Balazs Brankovics Anne D. van Diepeningen Katarzyna Bilska Maciej Żelechowski Kamil Myszczyński Kamil Myszczyński Tomasz Molcan Alexander Stakheev Sebastian Stenglein Sebastian Stenglein Marco Beyer Matias Pasquali Jakub Sawicki Joanna Wyrȩbek Anna Baturo-Cieśniewska |
| author_sort | Tomasz Kulik |
| collection | DOAJ |
| description | Much of the mitogenome variation observed in fungal lineages seems driven by mobile genetic elements (MGEs), which have invaded their genomes throughout evolution. The variation in the distribution and nucleotide diversity of these elements appears to be the main distinction between different fungal taxa, making them promising candidates for diagnostic purposes. Fungi of the genus Fusarium display a high variation in MGE content, from MGE-poor (Fusarium oxysporum and Fusarium fujikuroi species complex) to MGE-rich mitogenomes found in the important cereal pathogens F. culmorum and F. graminearum sensu stricto. In this study, we investigated the MGE variation in these latter two species by mitogenome analysis of geographically diverse strains. In addition, a smaller set of F. cerealis and F. pseudograminearum strains was included for comparison. Forty-seven introns harboring from 0 to 3 endonucleases (HEGs) were identified in the standard set of mitochondrial protein-coding genes. Most of them belonged to the group I intron family and harbored either LAGLIDADG or GIY-YIG HEGs. Among a total of 53 HEGs, 27 were shared by all fungal strains. Most of the optional HEGs were irregularly distributed among fungal strains/species indicating ancestral mosaicism in MGEs. However, among optional MGEs, one exhibited species-specific conservation in F. culmorum. While in F. graminearum s.s. MGE patterns in cox3 and in the intergenic spacer between cox2 and nad4L may facilitate the identification of this species. Thus, our results demonstrate distinctive traits of mitogenomes for diagnostic purposes of Fusaria. |
| first_indexed | 2024-12-22T08:26:22Z |
| format | Article |
| id | doaj.art-617a7fdc6379410e8907bf700b3f4038 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T08:26:22Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-617a7fdc6379410e8907bf700b3f40382022-12-21T18:32:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-05-011110.3389/fmicb.2020.01002536335Diversity of Mobile Genetic Elements in the Mitogenomes of Closely Related Fusarium culmorum and F. graminearum sensu stricto Strains and Its Implication for Diagnostic PurposesTomasz Kulik0Balazs Brankovics1Anne D. van Diepeningen2Katarzyna Bilska3Maciej Żelechowski4Kamil Myszczyński5Kamil Myszczyński6Tomasz Molcan7Alexander Stakheev8Sebastian Stenglein9Sebastian Stenglein10Marco Beyer11Matias Pasquali12Jakub Sawicki13Joanna Wyrȩbek14Anna Baturo-Cieśniewska15Department of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandBiointeractions & Plant Health, Wageningen Plant Research, Wageningen, NetherlandsBiointeractions & Plant Health, Wageningen Plant Research, Wageningen, NetherlandsDepartment of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandDepartment of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandDepartment of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandMolecular Biology Laboratory, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandShemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, RussiaNational Scientific and Technical Research Council, Godoy Cruz, ArgentinaUniversidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, ArgentinaDepartment of Environmental Research and Innovation, Agro-Environmental Systems, Luxembourg Institute of Science and Technology, Belval, LuxembourgDepartment of Food, Environmental and Nutritional Sciences, University of Milan, Milan, ItalyDepartment of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Olsztyn, PolandDepartment of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland0Laboratory of Phytopathology and Molecular Mycology, Department of Biology and Plant Protection, UTP University of Science and Technology, Bydgoszcz, PolandMuch of the mitogenome variation observed in fungal lineages seems driven by mobile genetic elements (MGEs), which have invaded their genomes throughout evolution. The variation in the distribution and nucleotide diversity of these elements appears to be the main distinction between different fungal taxa, making them promising candidates for diagnostic purposes. Fungi of the genus Fusarium display a high variation in MGE content, from MGE-poor (Fusarium oxysporum and Fusarium fujikuroi species complex) to MGE-rich mitogenomes found in the important cereal pathogens F. culmorum and F. graminearum sensu stricto. In this study, we investigated the MGE variation in these latter two species by mitogenome analysis of geographically diverse strains. In addition, a smaller set of F. cerealis and F. pseudograminearum strains was included for comparison. Forty-seven introns harboring from 0 to 3 endonucleases (HEGs) were identified in the standard set of mitochondrial protein-coding genes. Most of them belonged to the group I intron family and harbored either LAGLIDADG or GIY-YIG HEGs. Among a total of 53 HEGs, 27 were shared by all fungal strains. Most of the optional HEGs were irregularly distributed among fungal strains/species indicating ancestral mosaicism in MGEs. However, among optional MGEs, one exhibited species-specific conservation in F. culmorum. While in F. graminearum s.s. MGE patterns in cox3 and in the intergenic spacer between cox2 and nad4L may facilitate the identification of this species. Thus, our results demonstrate distinctive traits of mitogenomes for diagnostic purposes of Fusaria.https://www.frontiersin.org/article/10.3389/fmicb.2020.01002/fullFusarium graminearum sensu strictoF. culmorummitogenomemobile genetic elementsmitochondrial intronshoming endonucleases |
| spellingShingle | Tomasz Kulik Balazs Brankovics Anne D. van Diepeningen Katarzyna Bilska Maciej Żelechowski Kamil Myszczyński Kamil Myszczyński Tomasz Molcan Alexander Stakheev Sebastian Stenglein Sebastian Stenglein Marco Beyer Matias Pasquali Jakub Sawicki Joanna Wyrȩbek Anna Baturo-Cieśniewska Diversity of Mobile Genetic Elements in the Mitogenomes of Closely Related Fusarium culmorum and F. graminearum sensu stricto Strains and Its Implication for Diagnostic Purposes Frontiers in Microbiology Fusarium graminearum sensu stricto F. culmorum mitogenome mobile genetic elements mitochondrial introns homing endonucleases |
| title | Diversity of Mobile Genetic Elements in the Mitogenomes of Closely Related Fusarium culmorum and F. graminearum sensu stricto Strains and Its Implication for Diagnostic Purposes |
| title_full | Diversity of Mobile Genetic Elements in the Mitogenomes of Closely Related Fusarium culmorum and F. graminearum sensu stricto Strains and Its Implication for Diagnostic Purposes |
| title_fullStr | Diversity of Mobile Genetic Elements in the Mitogenomes of Closely Related Fusarium culmorum and F. graminearum sensu stricto Strains and Its Implication for Diagnostic Purposes |
| title_full_unstemmed | Diversity of Mobile Genetic Elements in the Mitogenomes of Closely Related Fusarium culmorum and F. graminearum sensu stricto Strains and Its Implication for Diagnostic Purposes |
| title_short | Diversity of Mobile Genetic Elements in the Mitogenomes of Closely Related Fusarium culmorum and F. graminearum sensu stricto Strains and Its Implication for Diagnostic Purposes |
| title_sort | diversity of mobile genetic elements in the mitogenomes of closely related fusarium culmorum and f graminearum sensu stricto strains and its implication for diagnostic purposes |
| topic | Fusarium graminearum sensu stricto F. culmorum mitogenome mobile genetic elements mitochondrial introns homing endonucleases |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.01002/full |
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