Fabrication and Characterisation of Calcium Sulphate Hemihydrate Enhanced with Zn- or B-Doped Hydroxyapatite Nanoparticles for Hard Tissue Restoration
A composite based on calcium sulphate hemihydrate enhanced with Zn- or B-doped hydroxyapatite nanoparticles was fabricated and evaluated for bone graft applications. The investigations of their structural and morphological properties were performed by X-ray diffraction (XRD), Fourier transform infra...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/15/2219 |
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| author | Adrian Ionut Nicoara Teodor Gabriel Voineagu Andrada Elena Alecu Bogdan Stefan Vasile Ioana Maior Anca Cojocaru Roxana Trusca Roxana Cristina Popescu |
| author_facet | Adrian Ionut Nicoara Teodor Gabriel Voineagu Andrada Elena Alecu Bogdan Stefan Vasile Ioana Maior Anca Cojocaru Roxana Trusca Roxana Cristina Popescu |
| author_sort | Adrian Ionut Nicoara |
| collection | DOAJ |
| description | A composite based on calcium sulphate hemihydrate enhanced with Zn- or B-doped hydroxyapatite nanoparticles was fabricated and evaluated for bone graft applications. The investigations of their structural and morphological properties were performed by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) spectroscopy techniques. To study the bioactive properties of the obtained composites, soaking tests in simulated body fluid (SBF) were performed. The results showed that the addition of 2% Zn results in an increase of 2.27% in crystallinity, while the addition of boron causes an increase of 5.61% compared to the undoped HAp sample. The crystallite size was found to be 10.69 ± 1.59 nm for HAp@B, and in the case of HAp@Zn, the size reaches 16.63 ± 1.83 nm, compared to HAp, whose crystallite size value was 19.44 ± 3.13 nm. The mechanical resistance of the samples doped with zinc was the highest and decreased by about 6% after immersion in SBF. Mixing HAp nanoparticles with gypsum improved cell viability compared to HAp for all concentrations (except for 200 µg/mL). Cell density decreased with increasing nanoparticle concentration, compared to gypsum, where the cell density was not significantly affected. The degree of cellular differentiation of osteoblast-type cells was more accentuated in the case of samples treated with G+HAp@B nanoparticles compared to HAp@B. Cell viability in these samples decreased inversely proportionally to the concentration of administered nanoparticles. From the point of view of cell density, this confirmed the quantitative data. |
| first_indexed | 2024-03-11T00:20:31Z |
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| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-11T00:20:31Z |
| publishDate | 2023-07-01 |
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| series | Nanomaterials |
| spelling | doaj.art-617a9dc5f21346659c64e785400763e62023-11-18T23:21:46ZengMDPI AGNanomaterials2079-49912023-07-011315221910.3390/nano13152219Fabrication and Characterisation of Calcium Sulphate Hemihydrate Enhanced with Zn- or B-Doped Hydroxyapatite Nanoparticles for Hard Tissue RestorationAdrian Ionut Nicoara0Teodor Gabriel Voineagu1Andrada Elena Alecu2Bogdan Stefan Vasile3Ioana Maior4Anca Cojocaru5Roxana Trusca6Roxana Cristina Popescu7Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, 060042 Bucharest, RomaniaFaculty of Medical Engineering, University Politehnica of Bucharest, 060042 Bucharest, RomaniaFaculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, 060042 Bucharest, RomaniaNational Research Center for Micro and Nanomaterials, University Politehnica of Bucharest, 060042 Bucharest, RomaniaFaculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, 060042 Bucharest, RomaniaFaculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, 060042 Bucharest, RomaniaFaculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, 060042 Bucharest, RomaniaFaculty of Medical Engineering, University Politehnica of Bucharest, 060042 Bucharest, RomaniaA composite based on calcium sulphate hemihydrate enhanced with Zn- or B-doped hydroxyapatite nanoparticles was fabricated and evaluated for bone graft applications. The investigations of their structural and morphological properties were performed by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) spectroscopy techniques. To study the bioactive properties of the obtained composites, soaking tests in simulated body fluid (SBF) were performed. The results showed that the addition of 2% Zn results in an increase of 2.27% in crystallinity, while the addition of boron causes an increase of 5.61% compared to the undoped HAp sample. The crystallite size was found to be 10.69 ± 1.59 nm for HAp@B, and in the case of HAp@Zn, the size reaches 16.63 ± 1.83 nm, compared to HAp, whose crystallite size value was 19.44 ± 3.13 nm. The mechanical resistance of the samples doped with zinc was the highest and decreased by about 6% after immersion in SBF. Mixing HAp nanoparticles with gypsum improved cell viability compared to HAp for all concentrations (except for 200 µg/mL). Cell density decreased with increasing nanoparticle concentration, compared to gypsum, where the cell density was not significantly affected. The degree of cellular differentiation of osteoblast-type cells was more accentuated in the case of samples treated with G+HAp@B nanoparticles compared to HAp@B. Cell viability in these samples decreased inversely proportionally to the concentration of administered nanoparticles. From the point of view of cell density, this confirmed the quantitative data.https://www.mdpi.com/2079-4991/13/15/2219nanomaterialsbone reparationgypsumdoped hydroxyapatitebone cement |
| spellingShingle | Adrian Ionut Nicoara Teodor Gabriel Voineagu Andrada Elena Alecu Bogdan Stefan Vasile Ioana Maior Anca Cojocaru Roxana Trusca Roxana Cristina Popescu Fabrication and Characterisation of Calcium Sulphate Hemihydrate Enhanced with Zn- or B-Doped Hydroxyapatite Nanoparticles for Hard Tissue Restoration Nanomaterials nanomaterials bone reparation gypsum doped hydroxyapatite bone cement |
| title | Fabrication and Characterisation of Calcium Sulphate Hemihydrate Enhanced with Zn- or B-Doped Hydroxyapatite Nanoparticles for Hard Tissue Restoration |
| title_full | Fabrication and Characterisation of Calcium Sulphate Hemihydrate Enhanced with Zn- or B-Doped Hydroxyapatite Nanoparticles for Hard Tissue Restoration |
| title_fullStr | Fabrication and Characterisation of Calcium Sulphate Hemihydrate Enhanced with Zn- or B-Doped Hydroxyapatite Nanoparticles for Hard Tissue Restoration |
| title_full_unstemmed | Fabrication and Characterisation of Calcium Sulphate Hemihydrate Enhanced with Zn- or B-Doped Hydroxyapatite Nanoparticles for Hard Tissue Restoration |
| title_short | Fabrication and Characterisation of Calcium Sulphate Hemihydrate Enhanced with Zn- or B-Doped Hydroxyapatite Nanoparticles for Hard Tissue Restoration |
| title_sort | fabrication and characterisation of calcium sulphate hemihydrate enhanced with zn or b doped hydroxyapatite nanoparticles for hard tissue restoration |
| topic | nanomaterials bone reparation gypsum doped hydroxyapatite bone cement |
| url | https://www.mdpi.com/2079-4991/13/15/2219 |
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