Use of ABA Treatment for the Activation of Drought Protective Mechanisms in Barley Under Non-stress Conditions
The present study evaluated the sensitivity of three different barley genotypes to stress simulated by the application of exogenous abscisic acid (20 µmol.l−1) at the early stage of the plant development. We used RIA method, instantaneous water use efficiency (WUE), the discrimination of 13C (Δ13C)...
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| Format: | Article |
| Language: | English |
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Mendel University Press
2015-01-01
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| Series: | Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis |
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| Online Access: | https://acta.mendelu.cz/63/1/0087/ |
| _version_ | 1811279043115352064 |
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| author | Lucie Melišová Marie Hronková Ludmila Holková Marek Klemš Pavlína Smutná |
| author_facet | Lucie Melišová Marie Hronková Ludmila Holková Marek Klemš Pavlína Smutná |
| author_sort | Lucie Melišová |
| collection | DOAJ |
| description | The present study evaluated the sensitivity of three different barley genotypes to stress simulated by the application of exogenous abscisic acid (20 µmol.l−1) at the early stage of the plant development. We used RIA method, instantaneous water use efficiency (WUE), the discrimination of 13C (Δ13C) and the expression levels of Dhn4 gene. The increase of ABA concentration in leaves after exogenous ABA application was detected in all tested genotypes; however, the lowest amount was found in the most tolerant genotype. Increased level of the instantaneous WUE after ABA treatment was found in all genotypes. The Δ13C in ABA treated plants decreased, however, relatively drought-tolerant genotype Tadmor showed lower discrimination even in control variant. The genotype-dependent differences were observed in the expression levels of Dhn4 gene in the leaves. High expression level of this gene was observed in Tadmor. Based on the exogenous ABA level, it was possible to distinguish two types of response of plants to exogenous ABA. Tadmor represented one of them as manifesting high sensitivity to exogenous ABA, leading to fast induction of Dhn4 gene relative expression. Conversely, spring genotypes of Jersey and Malz manifested slower response to exogenous ABA as well as lower WUE values and relative expression of Dhn4. The results supported the idea that ABA application may activate similar stress reactions in plants as drought conditions and additionally the intensity of this reaction is genotype dependent. |
| first_indexed | 2024-04-13T00:47:44Z |
| format | Article |
| id | doaj.art-617e0196190d4bea885474dec12cff5c |
| institution | Directory Open Access Journal |
| issn | 1211-8516 2464-8310 |
| language | English |
| last_indexed | 2024-04-13T00:47:44Z |
| publishDate | 2015-01-01 |
| publisher | Mendel University Press |
| record_format | Article |
| series | Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis |
| spelling | doaj.art-617e0196190d4bea885474dec12cff5c2022-12-22T03:09:57ZengMendel University PressActa Universitatis Agriculturae et Silviculturae Mendelianae Brunensis1211-85162464-83102015-01-01631879310.11118/actaun201563010087Use of ABA Treatment for the Activation of Drought Protective Mechanisms in Barley Under Non-stress ConditionsLucie Melišová0Marie Hronková1Ludmila Holková2Marek Klemš3Pavlína Smutná4Department of Crop Science, Breeding and Plant Medicine, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech RepublicBiology Centre AS CR, v.v.i., Institute of Plant Molecular Biology, Branišovská 31/1160, 370 05 České Budějovice, Czech RepublicDepartment of Crop Science, Breeding and Plant Medicine, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech RepublicDepartment of Plant Biology, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech RepublicDepartment of Crop Science, Breeding and Plant Medicine, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech RepublicThe present study evaluated the sensitivity of three different barley genotypes to stress simulated by the application of exogenous abscisic acid (20 µmol.l−1) at the early stage of the plant development. We used RIA method, instantaneous water use efficiency (WUE), the discrimination of 13C (Δ13C) and the expression levels of Dhn4 gene. The increase of ABA concentration in leaves after exogenous ABA application was detected in all tested genotypes; however, the lowest amount was found in the most tolerant genotype. Increased level of the instantaneous WUE after ABA treatment was found in all genotypes. The Δ13C in ABA treated plants decreased, however, relatively drought-tolerant genotype Tadmor showed lower discrimination even in control variant. The genotype-dependent differences were observed in the expression levels of Dhn4 gene in the leaves. High expression level of this gene was observed in Tadmor. Based on the exogenous ABA level, it was possible to distinguish two types of response of plants to exogenous ABA. Tadmor represented one of them as manifesting high sensitivity to exogenous ABA, leading to fast induction of Dhn4 gene relative expression. Conversely, spring genotypes of Jersey and Malz manifested slower response to exogenous ABA as well as lower WUE values and relative expression of Dhn4. The results supported the idea that ABA application may activate similar stress reactions in plants as drought conditions and additionally the intensity of this reaction is genotype dependent.https://acta.mendelu.cz/63/1/0087/Abscisic aciddrought stressDhngene expression<i>Hordeum vulgare</i> L.WUE |
| spellingShingle | Lucie Melišová Marie Hronková Ludmila Holková Marek Klemš Pavlína Smutná Use of ABA Treatment for the Activation of Drought Protective Mechanisms in Barley Under Non-stress Conditions Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis Abscisic acid drought stress Dhn gene expression <i>Hordeum vulgare</i> L. WUE |
| title | Use of ABA Treatment for the Activation of Drought Protective Mechanisms in Barley Under Non-stress Conditions |
| title_full | Use of ABA Treatment for the Activation of Drought Protective Mechanisms in Barley Under Non-stress Conditions |
| title_fullStr | Use of ABA Treatment for the Activation of Drought Protective Mechanisms in Barley Under Non-stress Conditions |
| title_full_unstemmed | Use of ABA Treatment for the Activation of Drought Protective Mechanisms in Barley Under Non-stress Conditions |
| title_short | Use of ABA Treatment for the Activation of Drought Protective Mechanisms in Barley Under Non-stress Conditions |
| title_sort | use of aba treatment for the activation of drought protective mechanisms in barley under non stress conditions |
| topic | Abscisic acid drought stress Dhn gene expression <i>Hordeum vulgare</i> L. WUE |
| url | https://acta.mendelu.cz/63/1/0087/ |
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