Antibacterial and antibiofilm activity of Lactobacillus strains secretome and extraction against Escherichia coli isolated from urinary tract infection
The purpose of this study was to antibacterial, and antibiofilm activity of two Lactobacillus strains secretome and extraction against E. coli isolated from women with urinary tract infection (UTI). We isolated 100 E. coli samples from women with UTI. Lactobacillus acidophilus and Lactobacillus case...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X22000583 |
| _version_ | 1811279355478802432 |
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| author | Nayemeh Soltani Samane Abbasi Sevda Baghaeifar Elham Taheri Mahdieh Farhoudi Sefidan Jadid Parisa Emami Kamilia Abolhasani Firouz Aslanshirzadeh |
| author_facet | Nayemeh Soltani Samane Abbasi Sevda Baghaeifar Elham Taheri Mahdieh Farhoudi Sefidan Jadid Parisa Emami Kamilia Abolhasani Firouz Aslanshirzadeh |
| author_sort | Nayemeh Soltani |
| collection | DOAJ |
| description | The purpose of this study was to antibacterial, and antibiofilm activity of two Lactobacillus strains secretome and extraction against E. coli isolated from women with urinary tract infection (UTI). We isolated 100 E. coli samples from women with UTI. Lactobacillus acidophilus and Lactobacillus casei characteristics were evaluated, and their secretome and extraction were prepared. The antibacterial and antibiofilm activity of secretome and extraction of both Lactobacillus strains were evaluated against isolated E. coli samples. L. acidophilus and L. casei were able to tolerate pH 3, bile salts, and pancreatic enzymes. Both probiotics were not resistant to antibiotics and demonstrated an appropriate ability to adhere to the intestinal epithelial cells. Secretome and extraction of L. acidophilus and L. casei strains showed a good antibacterial and antibiofilm against E. coli isolates. Generally, present study suggested that the secretome and extraction of L. acidophilus and L. casei strains exhibits a good antimicrobial activity. |
| first_indexed | 2024-04-13T00:53:08Z |
| format | Article |
| id | doaj.art-617e8a0b686c4d3c9c4af4ba10f3f760 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-13T00:53:08Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-617e8a0b686c4d3c9c4af4ba10f3f7602022-12-22T03:09:47ZengElsevierBiotechnology Reports2215-017X2022-12-0136e00760Antibacterial and antibiofilm activity of Lactobacillus strains secretome and extraction against Escherichia coli isolated from urinary tract infectionNayemeh Soltani0Samane Abbasi1Sevda Baghaeifar2Elham Taheri3Mahdieh Farhoudi Sefidan Jadid4Parisa Emami5Kamilia Abolhasani6Firouz Aslanshirzadeh7Department of Microbiology, Urmia Branch, Islamic Azad University, Urmia, IranDepartment of Biology, Faculty of Sciences, University of Guilan, Rasht, IranDepartment of Biotechnology, Urmia Branch, Islamic Azad University, Urmia, IranDepartment of Pharmaceutical Biotechnology, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Genetics, Tabriz Branch, Islamic Azad University, Branch, IranDepartment of Genetics, Ahar Branch, Islamic Azad University, Ahar, IranDepartment of Anesthesia, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Infectious Disease, Tabriz University of Medical Sciences, Tabriz, Iran; Corresponding author at: Department of Infectious Disease, School of Medicine, Tabriz University of Medical Sciences, Gholghasht Ave, Tabriz, Iran.The purpose of this study was to antibacterial, and antibiofilm activity of two Lactobacillus strains secretome and extraction against E. coli isolated from women with urinary tract infection (UTI). We isolated 100 E. coli samples from women with UTI. Lactobacillus acidophilus and Lactobacillus casei characteristics were evaluated, and their secretome and extraction were prepared. The antibacterial and antibiofilm activity of secretome and extraction of both Lactobacillus strains were evaluated against isolated E. coli samples. L. acidophilus and L. casei were able to tolerate pH 3, bile salts, and pancreatic enzymes. Both probiotics were not resistant to antibiotics and demonstrated an appropriate ability to adhere to the intestinal epithelial cells. Secretome and extraction of L. acidophilus and L. casei strains showed a good antibacterial and antibiofilm against E. coli isolates. Generally, present study suggested that the secretome and extraction of L. acidophilus and L. casei strains exhibits a good antimicrobial activity.http://www.sciencedirect.com/science/article/pii/S2215017X22000583Anti-bacterial agentsProbioticsLactobacillusUrinary tract infection |
| spellingShingle | Nayemeh Soltani Samane Abbasi Sevda Baghaeifar Elham Taheri Mahdieh Farhoudi Sefidan Jadid Parisa Emami Kamilia Abolhasani Firouz Aslanshirzadeh Antibacterial and antibiofilm activity of Lactobacillus strains secretome and extraction against Escherichia coli isolated from urinary tract infection Biotechnology Reports Anti-bacterial agents Probiotics Lactobacillus Urinary tract infection |
| title | Antibacterial and antibiofilm activity of Lactobacillus strains secretome and extraction against Escherichia coli isolated from urinary tract infection |
| title_full | Antibacterial and antibiofilm activity of Lactobacillus strains secretome and extraction against Escherichia coli isolated from urinary tract infection |
| title_fullStr | Antibacterial and antibiofilm activity of Lactobacillus strains secretome and extraction against Escherichia coli isolated from urinary tract infection |
| title_full_unstemmed | Antibacterial and antibiofilm activity of Lactobacillus strains secretome and extraction against Escherichia coli isolated from urinary tract infection |
| title_short | Antibacterial and antibiofilm activity of Lactobacillus strains secretome and extraction against Escherichia coli isolated from urinary tract infection |
| title_sort | antibacterial and antibiofilm activity of lactobacillus strains secretome and extraction against escherichia coli isolated from urinary tract infection |
| topic | Anti-bacterial agents Probiotics Lactobacillus Urinary tract infection |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X22000583 |
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