Studying the Polymorphism in the Gene Encoding Interleukin-17 [rs4711998 (A/G)] with PCR-RFLP in Patients with Brucellosis Compared with Healthy Subjects: A Case-Control Study
Background and purpose: Brucellosis is a systemic infection caused by gram-negative coccobacilli and facultative intracellular bacteria of the genus Brucella. Interleukin-17 is one of the important cytokines that plays a role in controlling host immune response in patients with brucellosis. The aim...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2017-12-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
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| Online Access: | http://jmums.mazums.ac.ir/article-1-8932-en.html |
| _version_ | 1828054788022992896 |
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| author | Hamed Farhadi Kohan Fariba Keramat Hassan Mahmoudi Alireza Zamani Massoud Saidijam Mohammad Ebrahim Ghaffari Sima Kazemi Mohammad Yousef Alikhani |
| author_facet | Hamed Farhadi Kohan Fariba Keramat Hassan Mahmoudi Alireza Zamani Massoud Saidijam Mohammad Ebrahim Ghaffari Sima Kazemi Mohammad Yousef Alikhani |
| author_sort | Hamed Farhadi Kohan |
| collection | DOAJ |
| description | Background and purpose: Brucellosis is a systemic infection caused by gram-negative coccobacilli and facultative intracellular bacteria of the genus Brucella. Interleukin-17 is one of the important cytokines that plays a role in controlling host immune response in patients with brucellosis. The aim of this study was to investigate polymorphism of the genes encoding IL-17 in patients with brucellosis compared to healthy subjects.
Materials and methods: This case-control study included 86 patients with brucellosis who were selected based on clinical symptoms, serology, culture and PCR results. The control group composed of 86 healthy people. The polymorphism gene encoding Interleukin-17 was evaluated in both groups by PCR-RELP method.
Results: Current study showed that the frequencies of AA [OR=0.047 (95%CI: 0.01-0.12)] and GG [OR=337.20 (95%CI: 20.49-5541.39] were significant at position -1998 (G/A) in both cases and controls [P-values (AA) and (GG) = 0.001]. But, the frequency of AA genotypes in the control group was greater than the frequency of GG genotype in patients. The odds ratio for catching brucellosis in people who have genotype GG was 41 times higher than those who have genotype AA.
Conclusion: Findings showed that, AA and AG genotypes at -1998 (A/G) position are more important. So, the risk of brucellosis in people with GG genotype at position -1998 was higher than that in people with AA genotype. In other words, people with AA genotype are more protected against brucellosis at this position. |
| first_indexed | 2024-04-10T20:28:32Z |
| format | Article |
| id | doaj.art-6183fb02699649ff8fe55fce4309e866 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:28:32Z |
| publishDate | 2017-12-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-6183fb02699649ff8fe55fce4309e8662023-01-25T07:43:27ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792017-12-01271551626Studying the Polymorphism in the Gene Encoding Interleukin-17 [rs4711998 (A/G)] with PCR-RFLP in Patients with Brucellosis Compared with Healthy Subjects: A Case-Control StudyHamed Farhadi Kohan0Fariba Keramat1Hassan Mahmoudi2Alireza Zamani3Massoud Saidijam4Mohammad Ebrahim Ghaffari5Sima Kazemi6Mohammad Yousef Alikhani7 Background and purpose: Brucellosis is a systemic infection caused by gram-negative coccobacilli and facultative intracellular bacteria of the genus Brucella. Interleukin-17 is one of the important cytokines that plays a role in controlling host immune response in patients with brucellosis. The aim of this study was to investigate polymorphism of the genes encoding IL-17 in patients with brucellosis compared to healthy subjects. Materials and methods: This case-control study included 86 patients with brucellosis who were selected based on clinical symptoms, serology, culture and PCR results. The control group composed of 86 healthy people. The polymorphism gene encoding Interleukin-17 was evaluated in both groups by PCR-RELP method. Results: Current study showed that the frequencies of AA [OR=0.047 (95%CI: 0.01-0.12)] and GG [OR=337.20 (95%CI: 20.49-5541.39] were significant at position -1998 (G/A) in both cases and controls [P-values (AA) and (GG) = 0.001]. But, the frequency of AA genotypes in the control group was greater than the frequency of GG genotype in patients. The odds ratio for catching brucellosis in people who have genotype GG was 41 times higher than those who have genotype AA. Conclusion: Findings showed that, AA and AG genotypes at -1998 (A/G) position are more important. So, the risk of brucellosis in people with GG genotype at position -1998 was higher than that in people with AA genotype. In other words, people with AA genotype are more protected against brucellosis at this position.http://jmums.mazums.ac.ir/article-1-8932-en.htmlinterleukin-17cytokinesbrucellosispolymorphism |
| spellingShingle | Hamed Farhadi Kohan Fariba Keramat Hassan Mahmoudi Alireza Zamani Massoud Saidijam Mohammad Ebrahim Ghaffari Sima Kazemi Mohammad Yousef Alikhani Studying the Polymorphism in the Gene Encoding Interleukin-17 [rs4711998 (A/G)] with PCR-RFLP in Patients with Brucellosis Compared with Healthy Subjects: A Case-Control Study Journal of Mazandaran University of Medical Sciences interleukin-17 cytokines brucellosis polymorphism |
| title | Studying the Polymorphism in the Gene Encoding Interleukin-17 [rs4711998 (A/G)] with PCR-RFLP in Patients with Brucellosis Compared with Healthy Subjects: A Case-Control Study |
| title_full | Studying the Polymorphism in the Gene Encoding Interleukin-17 [rs4711998 (A/G)] with PCR-RFLP in Patients with Brucellosis Compared with Healthy Subjects: A Case-Control Study |
| title_fullStr | Studying the Polymorphism in the Gene Encoding Interleukin-17 [rs4711998 (A/G)] with PCR-RFLP in Patients with Brucellosis Compared with Healthy Subjects: A Case-Control Study |
| title_full_unstemmed | Studying the Polymorphism in the Gene Encoding Interleukin-17 [rs4711998 (A/G)] with PCR-RFLP in Patients with Brucellosis Compared with Healthy Subjects: A Case-Control Study |
| title_short | Studying the Polymorphism in the Gene Encoding Interleukin-17 [rs4711998 (A/G)] with PCR-RFLP in Patients with Brucellosis Compared with Healthy Subjects: A Case-Control Study |
| title_sort | studying the polymorphism in the gene encoding interleukin 17 rs4711998 a g with pcr rflp in patients with brucellosis compared with healthy subjects a case control study |
| topic | interleukin-17 cytokines brucellosis polymorphism |
| url | http://jmums.mazums.ac.ir/article-1-8932-en.html |
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