Probiotic Streptococcus salivarius K12 Alleviates Radiation-Induced Oral Mucositis in Mice
BackgroundOral mucositis is the most common oral complication of cancer patients receiving radiotherapy and/or chemotherapy, leading to poor quality of life. Limitations of the current interventions on radiation-induced oral mucositis (RIOM) urge the development of novel therapeutics. Here, we evalu...
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.684824/full |
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| author | Yan Wang Jiatong Li Haonan Zhang Xin Zheng Jiantao Wang Xiaoyue Jia Xian Peng Qian Xie Jing Zou Liwei Zheng Jiyao Li Xuedong Zhou Xin Xu Xin Xu |
| author_facet | Yan Wang Jiatong Li Haonan Zhang Xin Zheng Jiantao Wang Xiaoyue Jia Xian Peng Qian Xie Jing Zou Liwei Zheng Jiyao Li Xuedong Zhou Xin Xu Xin Xu |
| author_sort | Yan Wang |
| collection | DOAJ |
| description | BackgroundOral mucositis is the most common oral complication of cancer patients receiving radiotherapy and/or chemotherapy, leading to poor quality of life. Limitations of the current interventions on radiation-induced oral mucositis (RIOM) urge the development of novel therapeutics. Here, we evaluated the treatment outcome of probiotic Streptococcus salivarius K12 on RIOM mice, and oral microbiota that is associated with the progress of RIOM was further investigated.MethodsAn experimental RIOM mouse model was established, and S. salivarius K12 was applied to the mouse oral cavity daily. Histological analyses were performed to evaluate the severity of oral mucositis and the treatment outcome of S. salivarius K12. The oral microbiota of mice was further analyzed by 16S rRNA sequencing, microbial culture and qPCR.Results Irradiation induced conspicuous mucositis in the oral cavity of mice. S. salivarius K12 treatment was beneficial for the healing of RIOM, as reflected by reduced ulcer size, increased basal layer epithelial cellularity and mucosal thickness, and elevated epithelial proliferation and attenuated apoptosis. RIOM mice presented significant oral microbial dysbiosis, with an overgrowth of oral anaerobes. S. salivarius K12 treatment reconstituted the oral microbiota and decreased the abundance of oral anaerobes of RIOM mice. In addition, S. salivarius K12 treatment inhibited NI1060 in Pasteurella genus and downregulated the expression of nitrate reductase.ConclusionsS. salivarius K12 treatment can alleviate RIOM and reconstituted the dysbiotic oral microbiota in mice. S. salivarius K12 may represent a promising adjuvant treatment to improve the quality of life of cancer patients receiving radiotherapy. |
| first_indexed | 2024-12-19T21:38:58Z |
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| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-19T21:38:58Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj.art-61844f2af8064cfcba83c596e6f133b72022-12-21T20:04:42ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-06-011210.3389/fimmu.2021.684824684824Probiotic Streptococcus salivarius K12 Alleviates Radiation-Induced Oral Mucositis in MiceYan Wang0Jiatong Li1Haonan Zhang2Xin Zheng3Jiantao Wang4Xiaoyue Jia5Xian Peng6Qian Xie7Jing Zou8Liwei Zheng9Jiyao Li10Xuedong Zhou11Xin Xu12Xin Xu13State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Biotherapy, Department of Lung Cancer Center and Department of Radiation Oncology, West China Hospital, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Endodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL, United StatesState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaClinical Research Center for Oral Diseases of Sichuan Province, Chengdu, ChinaBackgroundOral mucositis is the most common oral complication of cancer patients receiving radiotherapy and/or chemotherapy, leading to poor quality of life. Limitations of the current interventions on radiation-induced oral mucositis (RIOM) urge the development of novel therapeutics. Here, we evaluated the treatment outcome of probiotic Streptococcus salivarius K12 on RIOM mice, and oral microbiota that is associated with the progress of RIOM was further investigated.MethodsAn experimental RIOM mouse model was established, and S. salivarius K12 was applied to the mouse oral cavity daily. Histological analyses were performed to evaluate the severity of oral mucositis and the treatment outcome of S. salivarius K12. The oral microbiota of mice was further analyzed by 16S rRNA sequencing, microbial culture and qPCR.Results Irradiation induced conspicuous mucositis in the oral cavity of mice. S. salivarius K12 treatment was beneficial for the healing of RIOM, as reflected by reduced ulcer size, increased basal layer epithelial cellularity and mucosal thickness, and elevated epithelial proliferation and attenuated apoptosis. RIOM mice presented significant oral microbial dysbiosis, with an overgrowth of oral anaerobes. S. salivarius K12 treatment reconstituted the oral microbiota and decreased the abundance of oral anaerobes of RIOM mice. In addition, S. salivarius K12 treatment inhibited NI1060 in Pasteurella genus and downregulated the expression of nitrate reductase.ConclusionsS. salivarius K12 treatment can alleviate RIOM and reconstituted the dysbiotic oral microbiota in mice. S. salivarius K12 may represent a promising adjuvant treatment to improve the quality of life of cancer patients receiving radiotherapy.https://www.frontiersin.org/articles/10.3389/fimmu.2021.684824/fullStreptococcus salivarius K12radiotherapyoral mucositisdysbiosisprobiotics |
| spellingShingle | Yan Wang Jiatong Li Haonan Zhang Xin Zheng Jiantao Wang Xiaoyue Jia Xian Peng Qian Xie Jing Zou Liwei Zheng Jiyao Li Xuedong Zhou Xin Xu Xin Xu Probiotic Streptococcus salivarius K12 Alleviates Radiation-Induced Oral Mucositis in Mice Frontiers in Immunology Streptococcus salivarius K12 radiotherapy oral mucositis dysbiosis probiotics |
| title | Probiotic Streptococcus salivarius K12 Alleviates Radiation-Induced Oral Mucositis in Mice |
| title_full | Probiotic Streptococcus salivarius K12 Alleviates Radiation-Induced Oral Mucositis in Mice |
| title_fullStr | Probiotic Streptococcus salivarius K12 Alleviates Radiation-Induced Oral Mucositis in Mice |
| title_full_unstemmed | Probiotic Streptococcus salivarius K12 Alleviates Radiation-Induced Oral Mucositis in Mice |
| title_short | Probiotic Streptococcus salivarius K12 Alleviates Radiation-Induced Oral Mucositis in Mice |
| title_sort | probiotic streptococcus salivarius k12 alleviates radiation induced oral mucositis in mice |
| topic | Streptococcus salivarius K12 radiotherapy oral mucositis dysbiosis probiotics |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.684824/full |
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