Prunus mume Sieb. et Zucc. fruit ameliorates alcoholic liver injury in mice by inhibiting apoptosis and inflammation through oxidative stress
The effects of Prunus mume Sieb. et Zucc. extract (PME) on alcohol-induced liver injury were investigated. These functions of PME were linked to antioxidant activity that suppress lipogenesis in liver tissue, in part inhibiting activation of sterol response element binding protein-1 through ROS-mito...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-08-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464616300974 |
| _version_ | 1819044288832471040 |
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| author | Jeong Hoon Pan Ki Yong Lee Jun Ho Kim Hyeji Shin Jin Hyup Lee Young Jun Kim |
| author_facet | Jeong Hoon Pan Ki Yong Lee Jun Ho Kim Hyeji Shin Jin Hyup Lee Young Jun Kim |
| author_sort | Jeong Hoon Pan |
| collection | DOAJ |
| description | The effects of Prunus mume Sieb. et Zucc. extract (PME) on alcohol-induced liver injury were investigated. These functions of PME were linked to antioxidant activity that suppress lipogenesis in liver tissue, in part inhibiting activation of sterol response element binding protein-1 through ROS-mitogen-activated protein kinase signalling axis, and by suppressing hepatic apoptosis and inflammation through ROS-mediated p53 signalling pathway. Eight compounds and their structures in PME were also identified by LC-MS analysis, and chlorogenic acid (CGA) and neochlorogenic acid (NeoCGA) were validated using standard compounds by HPLC. The effects of CGA against cellular damage caused by alcohol-induced oxidative stress were confirmed using AML-12 cells. CGA significantly inhibited alcohol-induced apoptosis and MAPK activation in the cells. Moreover, antioxidant activities of CGA in the alcohol treatment were demonstrated regarding to cellular redox status and oxidative damage. These studies validated therapeutic value of PME in the prevention of alcoholic liver disease. |
| first_indexed | 2024-12-21T10:10:18Z |
| format | Article |
| id | doaj.art-6184df7de3f14be69e7728571afa3dd3 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-21T10:10:18Z |
| publishDate | 2016-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-6184df7de3f14be69e7728571afa3dd32022-12-21T19:07:44ZengElsevierJournal of Functional Foods1756-46462016-08-0125135148Prunus mume Sieb. et Zucc. fruit ameliorates alcoholic liver injury in mice by inhibiting apoptosis and inflammation through oxidative stressJeong Hoon Pan0Ki Yong Lee1Jun Ho Kim2Hyeji Shin3Jin Hyup Lee4Young Jun Kim5Department of Food and Biotechnology, Korea University, Sejong 30019, South KoreaCollege of Pharmacy, Korea University, Sejong 30019, South KoreaDepartment of Food and Biotechnology, Korea University, Sejong 30019, South KoreaCollege of Pharmacy, Korea University, Sejong 30019, South KoreaDepartment of Food and Biotechnology, Korea University, Sejong 30019, South Korea; Corresponding authors. Department of Food and Biotechnology, Korea University, #9-523, Sejong 30019, South Korea. Tel.: +82 44 860 1435; fax: +82 44 860 1780.Department of Food and Biotechnology, Korea University, Sejong 30019, South Korea; Corresponding authors. Department of Food and Biotechnology, Korea University, #9-523, Sejong 30019, South Korea. Tel.: +82 44 860 1435; fax: +82 44 860 1780.The effects of Prunus mume Sieb. et Zucc. extract (PME) on alcohol-induced liver injury were investigated. These functions of PME were linked to antioxidant activity that suppress lipogenesis in liver tissue, in part inhibiting activation of sterol response element binding protein-1 through ROS-mitogen-activated protein kinase signalling axis, and by suppressing hepatic apoptosis and inflammation through ROS-mediated p53 signalling pathway. Eight compounds and their structures in PME were also identified by LC-MS analysis, and chlorogenic acid (CGA) and neochlorogenic acid (NeoCGA) were validated using standard compounds by HPLC. The effects of CGA against cellular damage caused by alcohol-induced oxidative stress were confirmed using AML-12 cells. CGA significantly inhibited alcohol-induced apoptosis and MAPK activation in the cells. Moreover, antioxidant activities of CGA in the alcohol treatment were demonstrated regarding to cellular redox status and oxidative damage. These studies validated therapeutic value of PME in the prevention of alcoholic liver disease.http://www.sciencedirect.com/science/article/pii/S1756464616300974Prunus mume Sieb. et ZuccAlcoholic liver injuryHepatic apoptosisLipogenesisNatural antioxidant |
| spellingShingle | Jeong Hoon Pan Ki Yong Lee Jun Ho Kim Hyeji Shin Jin Hyup Lee Young Jun Kim Prunus mume Sieb. et Zucc. fruit ameliorates alcoholic liver injury in mice by inhibiting apoptosis and inflammation through oxidative stress Journal of Functional Foods Prunus mume Sieb. et Zucc Alcoholic liver injury Hepatic apoptosis Lipogenesis Natural antioxidant |
| title | Prunus mume Sieb. et Zucc. fruit ameliorates alcoholic liver injury in mice by inhibiting apoptosis and inflammation through oxidative stress |
| title_full | Prunus mume Sieb. et Zucc. fruit ameliorates alcoholic liver injury in mice by inhibiting apoptosis and inflammation through oxidative stress |
| title_fullStr | Prunus mume Sieb. et Zucc. fruit ameliorates alcoholic liver injury in mice by inhibiting apoptosis and inflammation through oxidative stress |
| title_full_unstemmed | Prunus mume Sieb. et Zucc. fruit ameliorates alcoholic liver injury in mice by inhibiting apoptosis and inflammation through oxidative stress |
| title_short | Prunus mume Sieb. et Zucc. fruit ameliorates alcoholic liver injury in mice by inhibiting apoptosis and inflammation through oxidative stress |
| title_sort | prunus mume sieb et zucc fruit ameliorates alcoholic liver injury in mice by inhibiting apoptosis and inflammation through oxidative stress |
| topic | Prunus mume Sieb. et Zucc Alcoholic liver injury Hepatic apoptosis Lipogenesis Natural antioxidant |
| url | http://www.sciencedirect.com/science/article/pii/S1756464616300974 |
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