Molecular Mapping of QTLs Conferring Fusarium Head Blight Resistance in Chinese Wheat Cultivar Jingzhou 66
Fusarium head blight (FHB) is a destructive disease of wheat (<i>Triticum aestivum</i> L.), which not only significantly reduces grain yield, but also affects end-use quality. Breeding wheat cultivars with high FHB resistance is the most effective way to control the disease. The Chinese...
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MDPI AG
2020-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/8/1021 |
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| author | Qing Xu Fuchao Xu Dandan Qin Meifang Li George Fedak Wenguang Cao Lijun Yang Jing Dong |
| author_facet | Qing Xu Fuchao Xu Dandan Qin Meifang Li George Fedak Wenguang Cao Lijun Yang Jing Dong |
| author_sort | Qing Xu |
| collection | DOAJ |
| description | Fusarium head blight (FHB) is a destructive disease of wheat (<i>Triticum aestivum</i> L.), which not only significantly reduces grain yield, but also affects end-use quality. Breeding wheat cultivars with high FHB resistance is the most effective way to control the disease. The Chinese wheat cultivar Jingzhou 66 (JZ66) shows moderately high FHB resistance; however, the genetic basis of its resistance is unknown. A doubled haploid (DH) population consisting 209 lines was developed from a cross of JZ66 and Aikang 58 (AK58), a FHB susceptible wheat cultivar, to identify quantitative trait loci (QTL) that contribute to the FHB resistance. Five field experiments were established across two consecutive crop seasons (2018 and 2019) to evaluate the DH lines and parents for FHB response. The parents and DH population were genotyped with the wheat 55K single-nucleotide polymorphism (SNP) assay. Six QTLs associated with FHB resistance in JZ66 were mapped on chromosome 2DS, 3AS, 3AL, 3DL, 4DS, and 5DL, respectively. Four of the QTL (<i>QFhb.hbaas-2DS</i>, <i>QFhb.hbaas-3AL</i>, <i>QFhb.hbaas-4DS</i>, and <i>QFhb.hbaas-5DL</i>) were detected in at least two environments, and the QTL on 3AL and 5DL might be new. The QTL with major effects, <i>QFhb.hbaas-2DS</i> and <i>QFhb.hbaas-4DS</i>, explained up to 36.2% and 17.6% of the phenotypic variance, and were co-localized with the plant semi-dwarfing loci <i>Rht8</i> and <i>Rht-D1.</i> The dwarfing <i>Rht8</i> allele significantly increased spike compactness (SC) and FHB susceptibility causing a larger effect on FHB response than <i>Rht-D1</i> observed in this study. PCR–based SNP markers for <i>QFhb.hbaas-2DS</i>, <i>QFhb.hbaas-3AL</i>, <i>QFhb.hbaas-4DS</i>, and <i>QFhb.hbaas-5DL</i>, were developed to facilitate their use in breeding for FHB resistance by marker-assisted selection. |
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| spelling | doaj.art-618ca48c2f714272b27c547cf1aad7e52023-11-20T09:57:35ZengMDPI AGPlants2223-77472020-08-0198102110.3390/plants9081021Molecular Mapping of QTLs Conferring Fusarium Head Blight Resistance in Chinese Wheat Cultivar Jingzhou 66Qing Xu0Fuchao Xu1Dandan Qin2Meifang Li3George Fedak4Wenguang Cao5Lijun Yang6Jing Dong7Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaOttawa Research Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, CanadaOttawa Research Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, CanadaHubei Key Laboratory of Crop Disease, Insect Pests and Weeds Control, Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaFusarium head blight (FHB) is a destructive disease of wheat (<i>Triticum aestivum</i> L.), which not only significantly reduces grain yield, but also affects end-use quality. Breeding wheat cultivars with high FHB resistance is the most effective way to control the disease. The Chinese wheat cultivar Jingzhou 66 (JZ66) shows moderately high FHB resistance; however, the genetic basis of its resistance is unknown. A doubled haploid (DH) population consisting 209 lines was developed from a cross of JZ66 and Aikang 58 (AK58), a FHB susceptible wheat cultivar, to identify quantitative trait loci (QTL) that contribute to the FHB resistance. Five field experiments were established across two consecutive crop seasons (2018 and 2019) to evaluate the DH lines and parents for FHB response. The parents and DH population were genotyped with the wheat 55K single-nucleotide polymorphism (SNP) assay. Six QTLs associated with FHB resistance in JZ66 were mapped on chromosome 2DS, 3AS, 3AL, 3DL, 4DS, and 5DL, respectively. Four of the QTL (<i>QFhb.hbaas-2DS</i>, <i>QFhb.hbaas-3AL</i>, <i>QFhb.hbaas-4DS</i>, and <i>QFhb.hbaas-5DL</i>) were detected in at least two environments, and the QTL on 3AL and 5DL might be new. The QTL with major effects, <i>QFhb.hbaas-2DS</i> and <i>QFhb.hbaas-4DS</i>, explained up to 36.2% and 17.6% of the phenotypic variance, and were co-localized with the plant semi-dwarfing loci <i>Rht8</i> and <i>Rht-D1.</i> The dwarfing <i>Rht8</i> allele significantly increased spike compactness (SC) and FHB susceptibility causing a larger effect on FHB response than <i>Rht-D1</i> observed in this study. PCR–based SNP markers for <i>QFhb.hbaas-2DS</i>, <i>QFhb.hbaas-3AL</i>, <i>QFhb.hbaas-4DS</i>, and <i>QFhb.hbaas-5DL</i>, were developed to facilitate their use in breeding for FHB resistance by marker-assisted selection.https://www.mdpi.com/2223-7747/9/8/1021<i>Fusarium graminearum</i>head scabQTL mapping<i>Triticum aestivum</i> |
| spellingShingle | Qing Xu Fuchao Xu Dandan Qin Meifang Li George Fedak Wenguang Cao Lijun Yang Jing Dong Molecular Mapping of QTLs Conferring Fusarium Head Blight Resistance in Chinese Wheat Cultivar Jingzhou 66 Plants <i>Fusarium graminearum</i> head scab QTL mapping <i>Triticum aestivum</i> |
| title | Molecular Mapping of QTLs Conferring Fusarium Head Blight Resistance in Chinese Wheat Cultivar Jingzhou 66 |
| title_full | Molecular Mapping of QTLs Conferring Fusarium Head Blight Resistance in Chinese Wheat Cultivar Jingzhou 66 |
| title_fullStr | Molecular Mapping of QTLs Conferring Fusarium Head Blight Resistance in Chinese Wheat Cultivar Jingzhou 66 |
| title_full_unstemmed | Molecular Mapping of QTLs Conferring Fusarium Head Blight Resistance in Chinese Wheat Cultivar Jingzhou 66 |
| title_short | Molecular Mapping of QTLs Conferring Fusarium Head Blight Resistance in Chinese Wheat Cultivar Jingzhou 66 |
| title_sort | molecular mapping of qtls conferring fusarium head blight resistance in chinese wheat cultivar jingzhou 66 |
| topic | <i>Fusarium graminearum</i> head scab QTL mapping <i>Triticum aestivum</i> |
| url | https://www.mdpi.com/2223-7747/9/8/1021 |
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