Biological Control of Fusarium oxysporum, the Causal Agent of Fusarium Basal Rot in Onion by Bacillus spp.
Fusarium oxysporum is the main pathogen causing Fusarium basal rot in onion (Allium cepa L.), which incurs significant yield losses before and after harvest. Among management strategies, biological control is an environmentally safe and sustainable alternative to chemical control. In this study, we...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2023-12-01
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| Series: | The Plant Pathology Journal |
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| Online Access: | http://ppjonline.org/upload/pdf/PPJ-OA-08-2023-0118.pdf |
| _version_ | 1827588015328854016 |
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| author | Jong-Hwan Shin Ha-Kyoung Lee Seong-Chan Lee You-Kyoung Han |
| author_facet | Jong-Hwan Shin Ha-Kyoung Lee Seong-Chan Lee You-Kyoung Han |
| author_sort | Jong-Hwan Shin |
| collection | DOAJ |
| description | Fusarium oxysporum is the main pathogen causing Fusarium basal rot in onion (Allium cepa L.), which incurs significant yield losses before and after harvest. Among management strategies, biological control is an environmentally safe and sustainable alternative to chemical control. In this study, we isolated and screened bacteria for antifungal activity against the basal rot pathogen F. oxysporum. Isolates 23-045, 23-046, 23-052, 23-055, and 23-056 significantly inhibited F. oxysporum mycelial growth and conidial germination. Isolates 23-045, 23-046, 23-052, and 23-056 suppressed the development of Fusarium basal rot in both onion seedlings and bulbs in pot and spray inoculation assays. Isolate 23-055 was effective in onion seedlings but exhibited weak inhibitory effect on onion bulbs. Based on analyses of the 16S rRNA and rpoB gene sequences together with morphological analysis, isolates 23-045, 23-046, 23-052, and 23-055 were identified as Bacillus thuringiensis, and isolate 23-056 as Bacillus toyonensis. All five bacterial isolates exhibited cellulolytic, proteolytic, and phosphate-solubilizing activity, which may contribute to their antagonistic activity against onion basal rot disease. Taken together B. thuringiensis 23-045, 23-046, 23-052, and 23-055 and B. toyonensis 23-056 have potential for the biological control of Fusarium basal rot in onion. |
| first_indexed | 2024-03-09T00:26:17Z |
| format | Article |
| id | doaj.art-6192e49f7e824ce2b2ce67b5318f72bb |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-03-09T00:26:17Z |
| publishDate | 2023-12-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-6192e49f7e824ce2b2ce67b5318f72bb2023-12-12T00:28:14ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802023-12-0139660061310.5423/PPJ.OA.08.2023.01182420Biological Control of Fusarium oxysporum, the Causal Agent of Fusarium Basal Rot in Onion by Bacillus spp.Jong-Hwan Shin0Ha-Kyoung Lee1Seong-Chan Lee2You-Kyoung Han3Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, KoreaHorticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, KoreaHorticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, KoreaHorticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, KoreaFusarium oxysporum is the main pathogen causing Fusarium basal rot in onion (Allium cepa L.), which incurs significant yield losses before and after harvest. Among management strategies, biological control is an environmentally safe and sustainable alternative to chemical control. In this study, we isolated and screened bacteria for antifungal activity against the basal rot pathogen F. oxysporum. Isolates 23-045, 23-046, 23-052, 23-055, and 23-056 significantly inhibited F. oxysporum mycelial growth and conidial germination. Isolates 23-045, 23-046, 23-052, and 23-056 suppressed the development of Fusarium basal rot in both onion seedlings and bulbs in pot and spray inoculation assays. Isolate 23-055 was effective in onion seedlings but exhibited weak inhibitory effect on onion bulbs. Based on analyses of the 16S rRNA and rpoB gene sequences together with morphological analysis, isolates 23-045, 23-046, 23-052, and 23-055 were identified as Bacillus thuringiensis, and isolate 23-056 as Bacillus toyonensis. All five bacterial isolates exhibited cellulolytic, proteolytic, and phosphate-solubilizing activity, which may contribute to their antagonistic activity against onion basal rot disease. Taken together B. thuringiensis 23-045, 23-046, 23-052, and 23-055 and B. toyonensis 23-056 have potential for the biological control of Fusarium basal rot in onion.http://ppjonline.org/upload/pdf/PPJ-OA-08-2023-0118.pdfbacillus thuringiensisbiological controlfusarium basal rotfusarium oxysporumonion disease |
| spellingShingle | Jong-Hwan Shin Ha-Kyoung Lee Seong-Chan Lee You-Kyoung Han Biological Control of Fusarium oxysporum, the Causal Agent of Fusarium Basal Rot in Onion by Bacillus spp. The Plant Pathology Journal bacillus thuringiensis biological control fusarium basal rot fusarium oxysporum onion disease |
| title | Biological Control of Fusarium oxysporum, the Causal Agent of Fusarium Basal Rot in Onion by Bacillus spp. |
| title_full | Biological Control of Fusarium oxysporum, the Causal Agent of Fusarium Basal Rot in Onion by Bacillus spp. |
| title_fullStr | Biological Control of Fusarium oxysporum, the Causal Agent of Fusarium Basal Rot in Onion by Bacillus spp. |
| title_full_unstemmed | Biological Control of Fusarium oxysporum, the Causal Agent of Fusarium Basal Rot in Onion by Bacillus spp. |
| title_short | Biological Control of Fusarium oxysporum, the Causal Agent of Fusarium Basal Rot in Onion by Bacillus spp. |
| title_sort | biological control of fusarium oxysporum the causal agent of fusarium basal rot in onion by bacillus spp |
| topic | bacillus thuringiensis biological control fusarium basal rot fusarium oxysporum onion disease |
| url | http://ppjonline.org/upload/pdf/PPJ-OA-08-2023-0118.pdf |
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