Chemical and biological studies of β-carotene after exposure to Cannabis sativa smoke
Considering the increase in consumption of Cannabis sativa and the use of the compound β-carotene (BC) as supplement, we investigated potential changes in the chemical and biological proprieties of BC after exposure to C. sativa smoke (CSS). Our results showed that the BC exposed to CSS underwent 98...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-01-01
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| Series: | Toxicology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750016300439 |
| _version_ | 1811222478262894592 |
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| author | Dulciana S. do Monte Jonh A. Bezerra Tenório Isla V.G.A. Bastos Fábio de S. Mendonça Joaquim E. Neto Teresinha G. da Silva Clécio S. Ramos |
| author_facet | Dulciana S. do Monte Jonh A. Bezerra Tenório Isla V.G.A. Bastos Fábio de S. Mendonça Joaquim E. Neto Teresinha G. da Silva Clécio S. Ramos |
| author_sort | Dulciana S. do Monte |
| collection | DOAJ |
| description | Considering the increase in consumption of Cannabis sativa and the use of the compound β-carotene (BC) as supplement, we investigated potential changes in the chemical and biological proprieties of BC after exposure to C. sativa smoke (CSS). Our results showed that the BC exposed to CSS underwent 98.8% degradation and suffered loss of its antiradical activity. The major degradation products identified were 3-hydroxy-2,4,4-trimethylpentyl)2-methylpropanoate and (2-ethyl-3-hydroxyhexyl)2-methylpropanoate compounds. These are found in higher levels in the exhalations of colorectal cancer patients and are similar to the toxic products associated with lipid peroxidation of polyunsaturated fatty acids. In toxicological assays using micro-crustacean Artemia salina the BC was non-toxic, while the BC degraded by CSS had a toxicity of LC50 = 397.35 μg/mL. In Wistar rats, females treated with BC degraded by CSS (BCCSS) showed whitish liver spots, alterations in liver weight and in bilirubin and alkaline phosphatase levels, and decrease in the number of leukocytes associated with atypical lymphocytosis. In male rats, there was an increase in the number of leukocytes when compared to the control group. In the histopathological analysis, the cortical region of the kidneys showed the presence of discrete amorphous eosinophilic material (cylinders) in the lumen of the proximate and distal convoluted tubules. In general, the BC in contact with CSS undergoes chemical changes and exhibits toxicity to rats and Artemia salina. Keywords: Cannabis sativa smoke, β-Carotene, Toxicity, Degradation |
| first_indexed | 2024-04-12T08:17:33Z |
| format | Article |
| id | doaj.art-6194d647ad94492f9efdebe9e41367fb |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-04-12T08:17:33Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-6194d647ad94492f9efdebe9e41367fb2022-12-22T03:40:44ZengElsevierToxicology Reports2214-75002016-01-013516522Chemical and biological studies of β-carotene after exposure to Cannabis sativa smokeDulciana S. do Monte0Jonh A. Bezerra Tenório1Isla V.G.A. Bastos2Fábio de S. Mendonça3Joaquim E. Neto4Teresinha G. da Silva5Clécio S. Ramos6Department of Chemistry, Federal Rural University of Pernambuco, Recife-Pe, BrazilDepartment of Chemistry, Federal Rural University of Pernambuco, Recife-Pe, BrazilAntibiotics Department, Federal University of Pernambuco, Recife-Pe, BrazilDepartment of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Recife-Pe, BrazilDepartment of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Recife-Pe, BrazilAntibiotics Department, Federal University of Pernambuco, Recife-Pe, BrazilDepartment of Chemistry, Federal Rural University of Pernambuco, Recife-Pe, Brazil; Corresponding author.Considering the increase in consumption of Cannabis sativa and the use of the compound β-carotene (BC) as supplement, we investigated potential changes in the chemical and biological proprieties of BC after exposure to C. sativa smoke (CSS). Our results showed that the BC exposed to CSS underwent 98.8% degradation and suffered loss of its antiradical activity. The major degradation products identified were 3-hydroxy-2,4,4-trimethylpentyl)2-methylpropanoate and (2-ethyl-3-hydroxyhexyl)2-methylpropanoate compounds. These are found in higher levels in the exhalations of colorectal cancer patients and are similar to the toxic products associated with lipid peroxidation of polyunsaturated fatty acids. In toxicological assays using micro-crustacean Artemia salina the BC was non-toxic, while the BC degraded by CSS had a toxicity of LC50 = 397.35 μg/mL. In Wistar rats, females treated with BC degraded by CSS (BCCSS) showed whitish liver spots, alterations in liver weight and in bilirubin and alkaline phosphatase levels, and decrease in the number of leukocytes associated with atypical lymphocytosis. In male rats, there was an increase in the number of leukocytes when compared to the control group. In the histopathological analysis, the cortical region of the kidneys showed the presence of discrete amorphous eosinophilic material (cylinders) in the lumen of the proximate and distal convoluted tubules. In general, the BC in contact with CSS undergoes chemical changes and exhibits toxicity to rats and Artemia salina. Keywords: Cannabis sativa smoke, β-Carotene, Toxicity, Degradationhttp://www.sciencedirect.com/science/article/pii/S2214750016300439 |
| spellingShingle | Dulciana S. do Monte Jonh A. Bezerra Tenório Isla V.G.A. Bastos Fábio de S. Mendonça Joaquim E. Neto Teresinha G. da Silva Clécio S. Ramos Chemical and biological studies of β-carotene after exposure to Cannabis sativa smoke Toxicology Reports |
| title | Chemical and biological studies of β-carotene after exposure to Cannabis sativa smoke |
| title_full | Chemical and biological studies of β-carotene after exposure to Cannabis sativa smoke |
| title_fullStr | Chemical and biological studies of β-carotene after exposure to Cannabis sativa smoke |
| title_full_unstemmed | Chemical and biological studies of β-carotene after exposure to Cannabis sativa smoke |
| title_short | Chemical and biological studies of β-carotene after exposure to Cannabis sativa smoke |
| title_sort | chemical and biological studies of β carotene after exposure to cannabis sativa smoke |
| url | http://www.sciencedirect.com/science/article/pii/S2214750016300439 |
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