<i>Trypanosoma cruzi</i> Parasite Burdens of Several Triatomine Species in Colombia
<i>Trypanosoma cruzi</i>, the causal agent of Chagas disease, is mainly transmitted by insects of the Triatominae subfamily. In Colombia, there are 26 triatomine species, and 16 of them are naturally infected with the parasite. The parasite loads of naturally infected vectors can be sign...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Tropical Medicine and Infectious Disease |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2414-6366/7/12/445 |
| _version_ | 1797455034741424128 |
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| author | Natalia Velásquez-Ortiz Carolina Hernández Omar Cantillo-Barraza Nathalia Ballesteros Lissa Cruz-Saavedra Giovanny Herrera Luz Stella Buitrago Hugo Soto Manuel Medina Jatney Palacio Marina Stella González Andrés Cuervo Gustavo Vallejo Liliana Zuleta Dueñas Plutarco Urbano Marina Muñoz Juan David Ramírez |
| author_facet | Natalia Velásquez-Ortiz Carolina Hernández Omar Cantillo-Barraza Nathalia Ballesteros Lissa Cruz-Saavedra Giovanny Herrera Luz Stella Buitrago Hugo Soto Manuel Medina Jatney Palacio Marina Stella González Andrés Cuervo Gustavo Vallejo Liliana Zuleta Dueñas Plutarco Urbano Marina Muñoz Juan David Ramírez |
| author_sort | Natalia Velásquez-Ortiz |
| collection | DOAJ |
| description | <i>Trypanosoma cruzi</i>, the causal agent of Chagas disease, is mainly transmitted by insects of the Triatominae subfamily. In Colombia, there are 26 triatomine species, and 16 of them are naturally infected with the parasite. The parasite loads of naturally infected vectors can be significant in targeting specific species that can affect the epidemiology of the disease. Studying their ecology and behavior is vital to understand their role in <i>T. cruzi</i> transmission dynamics. We evaluated the parasite loads of 182 field-collected triatomines corresponding to 10 species in 13 departments across Colombia. We standardized a methodology to quantify <i>T. cruzi</i> DNA in these insects. We obtained a LOD (limit of detection) of 3.05 p-eq/mL. The 82% of triatomines we evaluated were positive for <i>T. cruzi</i> infection, with loads ranging from hundreds to millions of equivalent parasites per milliliter. <i>Panstrongylus geniculatus</i>, <i>Rhodnius prolixus</i>, and <i>Triatoma dimidiata</i> were the species with the highest loads of <i>T. cruzi</i>; however, other species whose role as vectors is still unknown were also found with high loads of parasites. Our results suggest the relevance of secondary species for <i>T. cruzi</i> transmission in Colombia. We hope our data can help improve entomological surveillance and vector control programs in the country and the region. |
| first_indexed | 2024-03-09T15:46:41Z |
| format | Article |
| id | doaj.art-6196386c1c184ebaad50f37d93626abf |
| institution | Directory Open Access Journal |
| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-03-09T15:46:41Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Tropical Medicine and Infectious Disease |
| spelling | doaj.art-6196386c1c184ebaad50f37d93626abf2023-11-24T18:28:45ZengMDPI AGTropical Medicine and Infectious Disease2414-63662022-12-0171244510.3390/tropicalmed7120445<i>Trypanosoma cruzi</i> Parasite Burdens of Several Triatomine Species in ColombiaNatalia Velásquez-Ortiz0Carolina Hernández1Omar Cantillo-Barraza2Nathalia Ballesteros3Lissa Cruz-Saavedra4Giovanny Herrera5Luz Stella Buitrago6Hugo Soto7Manuel Medina8Jatney Palacio9Marina Stella González10Andrés Cuervo11Gustavo Vallejo12Liliana Zuleta Dueñas13Plutarco Urbano14Marina Muñoz15Juan David Ramírez16Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, ColombiaCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, ColombiaCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, ColombiaCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, ColombiaCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, ColombiaCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, ColombiaLaboratorio de Salud Pública del Meta, Villavicencio, ColombiaLaboratorio de Salud Pública del Cesar, Valledupar, ColombiaPrograma de Control de ETV, Secretaría de Salud de Boyacá, Tunja, ColombiaLaboratorio Departamental de Salud Pública del Departamento del Chocó, Quibdó, ColombiaLaboratorio de Salud Pública del Meta, Villavicencio, ColombiaSecretaría Departamental de Salud de Arauca, Arauca, ColombiaLaboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Universidad del Tolima, Ibagué, ColombiaGrupo de Vigilancia en Salud Pública, Secretaría de Salud de Casanare, Yopal, ColombiaGrupo de Investigaciones Biológicas de la Orinoquia, Universidad Internacional del Trópico Americano (Unitrópico), Yopal, ColombiaCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, ColombiaCentro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia<i>Trypanosoma cruzi</i>, the causal agent of Chagas disease, is mainly transmitted by insects of the Triatominae subfamily. In Colombia, there are 26 triatomine species, and 16 of them are naturally infected with the parasite. The parasite loads of naturally infected vectors can be significant in targeting specific species that can affect the epidemiology of the disease. Studying their ecology and behavior is vital to understand their role in <i>T. cruzi</i> transmission dynamics. We evaluated the parasite loads of 182 field-collected triatomines corresponding to 10 species in 13 departments across Colombia. We standardized a methodology to quantify <i>T. cruzi</i> DNA in these insects. We obtained a LOD (limit of detection) of 3.05 p-eq/mL. The 82% of triatomines we evaluated were positive for <i>T. cruzi</i> infection, with loads ranging from hundreds to millions of equivalent parasites per milliliter. <i>Panstrongylus geniculatus</i>, <i>Rhodnius prolixus</i>, and <i>Triatoma dimidiata</i> were the species with the highest loads of <i>T. cruzi</i>; however, other species whose role as vectors is still unknown were also found with high loads of parasites. Our results suggest the relevance of secondary species for <i>T. cruzi</i> transmission in Colombia. We hope our data can help improve entomological surveillance and vector control programs in the country and the region.https://www.mdpi.com/2414-6366/7/12/445<i>Trypanosoma cruzi</i>parasite loadtriatominesColombiaqPCR |
| spellingShingle | Natalia Velásquez-Ortiz Carolina Hernández Omar Cantillo-Barraza Nathalia Ballesteros Lissa Cruz-Saavedra Giovanny Herrera Luz Stella Buitrago Hugo Soto Manuel Medina Jatney Palacio Marina Stella González Andrés Cuervo Gustavo Vallejo Liliana Zuleta Dueñas Plutarco Urbano Marina Muñoz Juan David Ramírez <i>Trypanosoma cruzi</i> Parasite Burdens of Several Triatomine Species in Colombia Tropical Medicine and Infectious Disease <i>Trypanosoma cruzi</i> parasite load triatomines Colombia qPCR |
| title | <i>Trypanosoma cruzi</i> Parasite Burdens of Several Triatomine Species in Colombia |
| title_full | <i>Trypanosoma cruzi</i> Parasite Burdens of Several Triatomine Species in Colombia |
| title_fullStr | <i>Trypanosoma cruzi</i> Parasite Burdens of Several Triatomine Species in Colombia |
| title_full_unstemmed | <i>Trypanosoma cruzi</i> Parasite Burdens of Several Triatomine Species in Colombia |
| title_short | <i>Trypanosoma cruzi</i> Parasite Burdens of Several Triatomine Species in Colombia |
| title_sort | i trypanosoma cruzi i parasite burdens of several triatomine species in colombia |
| topic | <i>Trypanosoma cruzi</i> parasite load triatomines Colombia qPCR |
| url | https://www.mdpi.com/2414-6366/7/12/445 |
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