Association of Matrix Metalloproteinase-3 (MMP-3) Gene Methylation Status with the Risk of Developing Achilles Tendonitis: A Preliminary Study
INTRODUCTION: In this study, we aimed to examine the relationship between the MMP-3 gene promoter methylation status and the risk of developing Achilles tendinitis in endurance athletes. METHODS: A total of 20 endurance athletes with achille tendonitis and 20 sedantary controls were involved in the...
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| Format: | Article |
| Language: | English |
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Van Yuzuncu Yil University, School of Medicine
2023-01-01
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| Series: | Van Tıp Dergisi |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=vtd&un=VTD-88864 |
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| author | Ercan Tural Zulfinaz Betul Celik Esra Tekcan Sengul Tural |
| author_facet | Ercan Tural Zulfinaz Betul Celik Esra Tekcan Sengul Tural |
| author_sort | Ercan Tural |
| collection | DOAJ |
| description | INTRODUCTION: In this study, we aimed to examine the relationship between the MMP-3 gene promoter methylation status and the risk of developing Achilles tendinitis in endurance athletes. METHODS: A total of 20 endurance athletes with achille tendonitis and 20 sedantary controls were involved in the study. DNA isolation and bisulfite modification were performed from peripheral blood samples of all volunteers. Using methylation-specific primers designed for MMP-3 gene promoters, methylation profiles were analyzed by the methylation specific polymerase chain reaction method RESULTS: In the MMP-3 gene promoter methiation analysis, the methylation frequency was found to be 75% in athletes with Achilles tendinitis, while it was found to be 100% in the control group (p<0.05). MMP-3 promoter region methylation status in the patient group was found to be hypomethylated compared to the control group. DISCUSSION AND CONCLUSION: The hypomethylated status of the MMP-3 gene promoter may have a role in developing achille tendonitis in athletes. Furher and larges studies are needed to confirm our results. |
| first_indexed | 2024-03-09T07:14:15Z |
| format | Article |
| id | doaj.art-61981ec4b1c54ce69b3d49bf983a5376 |
| institution | Directory Open Access Journal |
| issn | 2587-0351 |
| language | English |
| last_indexed | 2024-03-09T07:14:15Z |
| publishDate | 2023-01-01 |
| publisher | Van Yuzuncu Yil University, School of Medicine |
| record_format | Article |
| series | Van Tıp Dergisi |
| spelling | doaj.art-61981ec4b1c54ce69b3d49bf983a53762023-12-03T08:50:38ZengVan Yuzuncu Yil University, School of MedicineVan Tıp Dergisi2587-03512023-01-01301222610.5505/vtd.2023.88864VTD-88864Association of Matrix Metalloproteinase-3 (MMP-3) Gene Methylation Status with the Risk of Developing Achilles Tendonitis: A Preliminary StudyErcan Tural0Zulfinaz Betul Celik1Esra Tekcan2Sengul Tural3Ondokuz Mayıs University Faculty of Health Science Department of Physical Therapy and Rehabilitation, Samsun TürkiyeSamsun University Faculty of Medicine, Department of Medical Biology, Samsun, TürkiyeOndokuz Mayis University Faculty of Medicine Departement of Medical Biology, Samsun, TürkiyeOndokuz Mayis University Faculty of Medicine Departement of Medical Biology, Samsun, TürkiyeINTRODUCTION: In this study, we aimed to examine the relationship between the MMP-3 gene promoter methylation status and the risk of developing Achilles tendinitis in endurance athletes. METHODS: A total of 20 endurance athletes with achille tendonitis and 20 sedantary controls were involved in the study. DNA isolation and bisulfite modification were performed from peripheral blood samples of all volunteers. Using methylation-specific primers designed for MMP-3 gene promoters, methylation profiles were analyzed by the methylation specific polymerase chain reaction method RESULTS: In the MMP-3 gene promoter methiation analysis, the methylation frequency was found to be 75% in athletes with Achilles tendinitis, while it was found to be 100% in the control group (p<0.05). MMP-3 promoter region methylation status in the patient group was found to be hypomethylated compared to the control group. DISCUSSION AND CONCLUSION: The hypomethylated status of the MMP-3 gene promoter may have a role in developing achille tendonitis in athletes. Furher and larges studies are needed to confirm our results.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=vtd&un=VTD-88864epigenomicstendonopathymethylation |
| spellingShingle | Ercan Tural Zulfinaz Betul Celik Esra Tekcan Sengul Tural Association of Matrix Metalloproteinase-3 (MMP-3) Gene Methylation Status with the Risk of Developing Achilles Tendonitis: A Preliminary Study Van Tıp Dergisi epigenomics tendonopathy methylation |
| title | Association of Matrix Metalloproteinase-3 (MMP-3) Gene Methylation Status with the Risk of Developing Achilles Tendonitis: A Preliminary Study |
| title_full | Association of Matrix Metalloproteinase-3 (MMP-3) Gene Methylation Status with the Risk of Developing Achilles Tendonitis: A Preliminary Study |
| title_fullStr | Association of Matrix Metalloproteinase-3 (MMP-3) Gene Methylation Status with the Risk of Developing Achilles Tendonitis: A Preliminary Study |
| title_full_unstemmed | Association of Matrix Metalloproteinase-3 (MMP-3) Gene Methylation Status with the Risk of Developing Achilles Tendonitis: A Preliminary Study |
| title_short | Association of Matrix Metalloproteinase-3 (MMP-3) Gene Methylation Status with the Risk of Developing Achilles Tendonitis: A Preliminary Study |
| title_sort | association of matrix metalloproteinase 3 mmp 3 gene methylation status with the risk of developing achilles tendonitis a preliminary study |
| topic | epigenomics tendonopathy methylation |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=vtd&un=VTD-88864 |
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