Molecular Analysis of Antimicrobial Resistance among <i>Enterobacteriaceae</i> Isolated from Diarrhoeic Calves in Egypt
The present study was designed to investigate the presence of genes that conferred resistance to antimicrobials among <i>Enterobacteriaceae</i> that were isolated from diarrhoeic calves. A total of 120 faecal samples were collected from diarrhoeic calves that were raised in Kafr El-Sheik...
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MDPI AG
2021-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/6/1712 |
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| author | Abdel-Moamen E. Meshref Ibrahim E. Eldesoukey Abdulaziz S. Alouffi Saleh A. Alrashedi Salama A. Osman Ashraf M. Ahmed |
| author_facet | Abdel-Moamen E. Meshref Ibrahim E. Eldesoukey Abdulaziz S. Alouffi Saleh A. Alrashedi Salama A. Osman Ashraf M. Ahmed |
| author_sort | Abdel-Moamen E. Meshref |
| collection | DOAJ |
| description | The present study was designed to investigate the presence of genes that conferred resistance to antimicrobials among <i>Enterobacteriaceae</i> that were isolated from diarrhoeic calves. A total of 120 faecal samples were collected from diarrhoeic calves that were raised in Kafr El-Sheikh governorate, Egypt. The samples were screened for <i>Enterobacteriaceae</i>. A total of 149 isolates of bacteria were recovered and identified; <i>Escherichia coli</i> was found to be the most overwhelming species, followed by <i>Citrobacter diversus</i>, <i>Shigella</i> spp., <i>Serratia</i> spp., <i>Providencia</i> spp., <i>Enterobacter</i> spp., <i>Klebsiella pneumoniae</i>, <i>Proteus</i> spp., <i>Klebsiella oxytoca</i>, and <i>Morganella morganii</i>. All isolates were tested for susceptibility to 12 antimicrobials; resistant and intermediately resistant strains were screened by conventional polymerase chain reaction for the presence of antimicrobial resistance genes. Of the 149 isolates, 37 (24.8%) exhibited multidrug resistant phenotypes. The most prevalent multidrug resistant species were <i>E. coli</i>, <i>C. diversus</i>, <i>Serratia</i> spp., <i>K. pneumoniae</i>, <i>Shigella</i> spp., <i>Providencia</i> spp., and <i>K. oxytoca</i>. Class 1 integrons were detected in 28 (18.8%) isolates. All isolates were negative for class 2 integrons. The <i>bla</i><sub>TEM</sub> gene was identified in 37 (24.8%) isolates, whereas no isolates carried the <i>bla</i><sub>CTX-M</sub> gene<sub>.</sub> The florfenicol gene (<i>flo</i>R) was detected in two bacterial isolates (1.3%). The findings of this study reveal that calves may act as potential reservoirs of multidrug resistant bacteria that can be easily transmitted to humans. |
| first_indexed | 2024-03-10T10:36:07Z |
| format | Article |
| id | doaj.art-6198c832deb54bab9d8d2c75dd509531 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T10:36:07Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-6198c832deb54bab9d8d2c75dd5095312023-11-21T23:15:47ZengMDPI AGAnimals2076-26152021-06-01116171210.3390/ani11061712Molecular Analysis of Antimicrobial Resistance among <i>Enterobacteriaceae</i> Isolated from Diarrhoeic Calves in EgyptAbdel-Moamen E. Meshref0Ibrahim E. Eldesoukey1Abdulaziz S. Alouffi2Saleh A. Alrashedi3Salama A. Osman4Ashraf M. Ahmed5Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, EgyptDepartment of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, EgyptKing Abdulaziz City for Science and Technology, Riyadh 11442, Saudi ArabiaCentral Laboratory at Al Watania Poultry Company, Riyadh 51441, Saudi ArabiaDepartment of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, EgyptDepartment of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, EgyptThe present study was designed to investigate the presence of genes that conferred resistance to antimicrobials among <i>Enterobacteriaceae</i> that were isolated from diarrhoeic calves. A total of 120 faecal samples were collected from diarrhoeic calves that were raised in Kafr El-Sheikh governorate, Egypt. The samples were screened for <i>Enterobacteriaceae</i>. A total of 149 isolates of bacteria were recovered and identified; <i>Escherichia coli</i> was found to be the most overwhelming species, followed by <i>Citrobacter diversus</i>, <i>Shigella</i> spp., <i>Serratia</i> spp., <i>Providencia</i> spp., <i>Enterobacter</i> spp., <i>Klebsiella pneumoniae</i>, <i>Proteus</i> spp., <i>Klebsiella oxytoca</i>, and <i>Morganella morganii</i>. All isolates were tested for susceptibility to 12 antimicrobials; resistant and intermediately resistant strains were screened by conventional polymerase chain reaction for the presence of antimicrobial resistance genes. Of the 149 isolates, 37 (24.8%) exhibited multidrug resistant phenotypes. The most prevalent multidrug resistant species were <i>E. coli</i>, <i>C. diversus</i>, <i>Serratia</i> spp., <i>K. pneumoniae</i>, <i>Shigella</i> spp., <i>Providencia</i> spp., and <i>K. oxytoca</i>. Class 1 integrons were detected in 28 (18.8%) isolates. All isolates were negative for class 2 integrons. The <i>bla</i><sub>TEM</sub> gene was identified in 37 (24.8%) isolates, whereas no isolates carried the <i>bla</i><sub>CTX-M</sub> gene<sub>.</sub> The florfenicol gene (<i>flo</i>R) was detected in two bacterial isolates (1.3%). The findings of this study reveal that calves may act as potential reservoirs of multidrug resistant bacteria that can be easily transmitted to humans.https://www.mdpi.com/2076-2615/11/6/1712antimicrobial resistance<i>Enterobacteriaceae</i>calvesdiarrhoea |
| spellingShingle | Abdel-Moamen E. Meshref Ibrahim E. Eldesoukey Abdulaziz S. Alouffi Saleh A. Alrashedi Salama A. Osman Ashraf M. Ahmed Molecular Analysis of Antimicrobial Resistance among <i>Enterobacteriaceae</i> Isolated from Diarrhoeic Calves in Egypt Animals antimicrobial resistance <i>Enterobacteriaceae</i> calves diarrhoea |
| title | Molecular Analysis of Antimicrobial Resistance among <i>Enterobacteriaceae</i> Isolated from Diarrhoeic Calves in Egypt |
| title_full | Molecular Analysis of Antimicrobial Resistance among <i>Enterobacteriaceae</i> Isolated from Diarrhoeic Calves in Egypt |
| title_fullStr | Molecular Analysis of Antimicrobial Resistance among <i>Enterobacteriaceae</i> Isolated from Diarrhoeic Calves in Egypt |
| title_full_unstemmed | Molecular Analysis of Antimicrobial Resistance among <i>Enterobacteriaceae</i> Isolated from Diarrhoeic Calves in Egypt |
| title_short | Molecular Analysis of Antimicrobial Resistance among <i>Enterobacteriaceae</i> Isolated from Diarrhoeic Calves in Egypt |
| title_sort | molecular analysis of antimicrobial resistance among i enterobacteriaceae i isolated from diarrhoeic calves in egypt |
| topic | antimicrobial resistance <i>Enterobacteriaceae</i> calves diarrhoea |
| url | https://www.mdpi.com/2076-2615/11/6/1712 |
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