Electrochemical Enrichment and Isolation of Electrogenic Bacteria from 0.22 µm Filtrate
Ultramicrobacteria (UMB) that can pass through a 0.22 µm filter are attractive because of their novelty and diversity. However, isolating UMB has been difficult because of their symbiotic or parasitic lifestyles in the environment. Some UMB have extracellular electron transfer (EET)-related genes, s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/10/2051 |
| _version_ | 1797471070258724864 |
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| author | Sota Ihara Satoshi Wakai Tomoko Maehara Akihiro Okamoto |
| author_facet | Sota Ihara Satoshi Wakai Tomoko Maehara Akihiro Okamoto |
| author_sort | Sota Ihara |
| collection | DOAJ |
| description | Ultramicrobacteria (UMB) that can pass through a 0.22 µm filter are attractive because of their novelty and diversity. However, isolating UMB has been difficult because of their symbiotic or parasitic lifestyles in the environment. Some UMB have extracellular electron transfer (EET)-related genes, suggesting that these symbionts may grow on an electrode surface independently. Here, we attempted to culture from soil samples bacteria that passed through a 0.22 µm filter poised with +0.2 V vs. Ag/AgCl and isolated <i>Cellulomonas</i> sp. strain NTE-D12 from the electrochemical reactor. A phylogenetic analysis of the 16S rRNA showed 97.9% similarity to the closest related species, <i>Cellulomonas</i> <i>algicola</i>, indicating that the strain NTE-D12 is a novel species. Electrochemical and genomic analyses showed that the strain NTE-D12 generated the highest current density compared to that in the three related species, indicating the presence of a unique electron transfer system in the strain. Therefore, the present study provides a new isolation scheme for cultivating and isolating novel UMB potentially with a symbiotic relationship associated with interspecies electron transfer. |
| first_indexed | 2024-03-09T19:44:22Z |
| format | Article |
| id | doaj.art-619a7b3d999a46bb9bf5e6b51c9638f5 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T19:44:22Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-619a7b3d999a46bb9bf5e6b51c9638f52023-11-24T01:27:37ZengMDPI AGMicroorganisms2076-26072022-10-011010205110.3390/microorganisms10102051Electrochemical Enrichment and Isolation of Electrogenic Bacteria from 0.22 µm FiltrateSota Ihara0Satoshi Wakai1Tomoko Maehara2Akihiro Okamoto3Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8577, JapanInstitute for Extra-Cutting-Edge Science and Technology Avant-Garde Research (X-Star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Kanagawa 237-0061, JapanInternational Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), Ibaraki 305-0044, JapanInternational Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), Ibaraki 305-0044, JapanUltramicrobacteria (UMB) that can pass through a 0.22 µm filter are attractive because of their novelty and diversity. However, isolating UMB has been difficult because of their symbiotic or parasitic lifestyles in the environment. Some UMB have extracellular electron transfer (EET)-related genes, suggesting that these symbionts may grow on an electrode surface independently. Here, we attempted to culture from soil samples bacteria that passed through a 0.22 µm filter poised with +0.2 V vs. Ag/AgCl and isolated <i>Cellulomonas</i> sp. strain NTE-D12 from the electrochemical reactor. A phylogenetic analysis of the 16S rRNA showed 97.9% similarity to the closest related species, <i>Cellulomonas</i> <i>algicola</i>, indicating that the strain NTE-D12 is a novel species. Electrochemical and genomic analyses showed that the strain NTE-D12 generated the highest current density compared to that in the three related species, indicating the presence of a unique electron transfer system in the strain. Therefore, the present study provides a new isolation scheme for cultivating and isolating novel UMB potentially with a symbiotic relationship associated with interspecies electron transfer.https://www.mdpi.com/2076-2607/10/10/2051extracellular electron transferinterspecies electron transferultramicrobacteria<i>Cellulomonas</i> |
| spellingShingle | Sota Ihara Satoshi Wakai Tomoko Maehara Akihiro Okamoto Electrochemical Enrichment and Isolation of Electrogenic Bacteria from 0.22 µm Filtrate Microorganisms extracellular electron transfer interspecies electron transfer ultramicrobacteria <i>Cellulomonas</i> |
| title | Electrochemical Enrichment and Isolation of Electrogenic Bacteria from 0.22 µm Filtrate |
| title_full | Electrochemical Enrichment and Isolation of Electrogenic Bacteria from 0.22 µm Filtrate |
| title_fullStr | Electrochemical Enrichment and Isolation of Electrogenic Bacteria from 0.22 µm Filtrate |
| title_full_unstemmed | Electrochemical Enrichment and Isolation of Electrogenic Bacteria from 0.22 µm Filtrate |
| title_short | Electrochemical Enrichment and Isolation of Electrogenic Bacteria from 0.22 µm Filtrate |
| title_sort | electrochemical enrichment and isolation of electrogenic bacteria from 0 22 µm filtrate |
| topic | extracellular electron transfer interspecies electron transfer ultramicrobacteria <i>Cellulomonas</i> |
| url | https://www.mdpi.com/2076-2607/10/10/2051 |
| work_keys_str_mv | AT sotaihara electrochemicalenrichmentandisolationofelectrogenicbacteriafrom022μmfiltrate AT satoshiwakai electrochemicalenrichmentandisolationofelectrogenicbacteriafrom022μmfiltrate AT tomokomaehara electrochemicalenrichmentandisolationofelectrogenicbacteriafrom022μmfiltrate AT akihirookamoto electrochemicalenrichmentandisolationofelectrogenicbacteriafrom022μmfiltrate |