Following up of the Function of Acidithiodabacillus Ferrooxidans and Acidithiobacillus Thiooxidans Involved in Uranium Bioleaching After Preservation at Low Temperatures

Acidithiobacillus species is used in bioleaching process of metals such as uranium, copper, nickel. The purpose of this study was to develop an effective protocol using facilities available for preservation of isolated native bacteria involved in uranium bioleaching. In this study, the bacteria invo...

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Main Authors: F Fatemi, S Jahani, M. A Firooze-e-Zare
Format: Article
Language:fas
Published: Nuclear Science and Technology Research Institute 2016-11-01
Series:مجله علوم و فنون هسته‌ای
Subjects:
Online Access:https://jonsat.nstri.ir/article_122_15ee168d6a465f562f8241c0219629b3.pdf
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author F Fatemi
S Jahani
M. A Firooze-e-Zare
author_facet F Fatemi
S Jahani
M. A Firooze-e-Zare
author_sort F Fatemi
collection DOAJ
description Acidithiobacillus species is used in bioleaching process of metals such as uranium, copper, nickel. The purpose of this study was to develop an effective protocol using facilities available for preservation of isolated native bacteria involved in uranium bioleaching. In this study, the bacteria involved in uranium bioleaching AEOI  Laboratory were cultured, purified and stored at 4 and -80°C. Then, variation of pH, Eh, growth and biochemical characteristics were examined after 6 months of storage and compared with the bacteria before using the preservation methods. The results indicated that the preservation of the bacteria within 6 months at selective temperatures did not have any effect on the biochemical characteristics of the bacteria. In addition, no significant changes were observed in the variation of pH, Eh and growth of bacteria, before and after the preservations. The results suggested that 6 months preservations of native bacteria at 4 and -80°C, had no effect on the ability of bacteria in bioleaching process.
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spelling doaj.art-619beb8be2b34064b353b353f45b2e302023-05-02T10:24:16ZfasNuclear Science and Technology Research Instituteمجله علوم و فنون هسته‌ای1735-18712676-58612016-11-01373917122Following up of the Function of Acidithiodabacillus Ferrooxidans and Acidithiobacillus Thiooxidans Involved in Uranium Bioleaching After Preservation at Low TemperaturesF Fatemi0S Jahani1M. A Firooze-e-Zare2پژوهشگاه علوم و فنون هسته‌ای، سازمان انرژی اتمی ایران،پژوهشکده‌ی چرخه‌ی سوخت هسته‌ای، پژوهشگاه علوم و فنون هسته‌ایپژوهشکده‌ی چرخه‌ی سوخت هسته‌ای، پژوهشگاه علوم و فنون هسته‌ایAcidithiobacillus species is used in bioleaching process of metals such as uranium, copper, nickel. The purpose of this study was to develop an effective protocol using facilities available for preservation of isolated native bacteria involved in uranium bioleaching. In this study, the bacteria involved in uranium bioleaching AEOI  Laboratory were cultured, purified and stored at 4 and -80°C. Then, variation of pH, Eh, growth and biochemical characteristics were examined after 6 months of storage and compared with the bacteria before using the preservation methods. The results indicated that the preservation of the bacteria within 6 months at selective temperatures did not have any effect on the biochemical characteristics of the bacteria. In addition, no significant changes were observed in the variation of pH, Eh and growth of bacteria, before and after the preservations. The results suggested that 6 months preservations of native bacteria at 4 and -80°C, had no effect on the ability of bacteria in bioleaching process.https://jonsat.nstri.ir/article_122_15ee168d6a465f562f8241c0219629b3.pdfacidithiodabacillusbioleachingpreservationuraniumbacterial function
spellingShingle F Fatemi
S Jahani
M. A Firooze-e-Zare
Following up of the Function of Acidithiodabacillus Ferrooxidans and Acidithiobacillus Thiooxidans Involved in Uranium Bioleaching After Preservation at Low Temperatures
مجله علوم و فنون هسته‌ای
acidithiodabacillus
bioleaching
preservation
uranium
bacterial function
title Following up of the Function of Acidithiodabacillus Ferrooxidans and Acidithiobacillus Thiooxidans Involved in Uranium Bioleaching After Preservation at Low Temperatures
title_full Following up of the Function of Acidithiodabacillus Ferrooxidans and Acidithiobacillus Thiooxidans Involved in Uranium Bioleaching After Preservation at Low Temperatures
title_fullStr Following up of the Function of Acidithiodabacillus Ferrooxidans and Acidithiobacillus Thiooxidans Involved in Uranium Bioleaching After Preservation at Low Temperatures
title_full_unstemmed Following up of the Function of Acidithiodabacillus Ferrooxidans and Acidithiobacillus Thiooxidans Involved in Uranium Bioleaching After Preservation at Low Temperatures
title_short Following up of the Function of Acidithiodabacillus Ferrooxidans and Acidithiobacillus Thiooxidans Involved in Uranium Bioleaching After Preservation at Low Temperatures
title_sort following up of the function of acidithiodabacillus ferrooxidans and acidithiobacillus thiooxidans involved in uranium bioleaching after preservation at low temperatures
topic acidithiodabacillus
bioleaching
preservation
uranium
bacterial function
url https://jonsat.nstri.ir/article_122_15ee168d6a465f562f8241c0219629b3.pdf
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