Piezo‐assisted ICSI improves fertilization and blastocyst development rates compared with conventional ICSI in women aged more than 35 years
Abstract Purpose Piezo‐assisted intracytoplasmic sperm injection (Piezo‐ICSI) is reported to be an effective method for inseminating fragile oocytes compared with conventional ICSI (c‐ICSI). However, infertile patient groups suitable for Piezo‐ICSI have not been elucidated. This study was conducted...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-10-01
|
| Series: | Reproductive Medicine and Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/rmb2.12290 |
| _version_ | 1828421589642772480 |
|---|---|
| author | Kohyu Furuhashi Yoshimi Saeki Noritoshi Enatsu Toshiroh Iwasaki Koichi Ito Yuri Mizusawa Yukiko Matsumoto Shoji Kokeguchi Masahide Shiotani |
| author_facet | Kohyu Furuhashi Yoshimi Saeki Noritoshi Enatsu Toshiroh Iwasaki Koichi Ito Yuri Mizusawa Yukiko Matsumoto Shoji Kokeguchi Masahide Shiotani |
| author_sort | Kohyu Furuhashi |
| collection | DOAJ |
| description | Abstract Purpose Piezo‐assisted intracytoplasmic sperm injection (Piezo‐ICSI) is reported to be an effective method for inseminating fragile oocytes compared with conventional ICSI (c‐ICSI). However, infertile patient groups suitable for Piezo‐ICSI have not been elucidated. This study was conducted to determine age groups suitable for Piezo‐ICSI using sibling egg controls inseminated by a well‐trained embryologist to reduce technical inequalities. Methods A total of 947 matured oocytes were inseminated either by Piezo‐ICSI or by c‐ICSI in sibling oocytes as controls. Fertilization (2 pronuclei, PN), survival, and blastocyst development rates on day (D) 5 and D6 after insemination were compared between the Piezo‐ICSI and c‐ICSI groups. Further analyses were applied to groups of women >35 or ≤35 years of age. Results There were no significant differences in fertilization, survival, or blastocyst development rates between the two insemination treatment groups. However, for women >35 years of age, the fertilization (P = .008) and blastocyst development (P = .016) rates with Piezo‐ICSI on D5 and D6 were significantly higher than in those subjected to c‐ICSI. Conclusions Piezo‐ICSI was useful for inseminating oocytes from women >35 years of age. |
| first_indexed | 2024-12-10T15:33:42Z |
| format | Article |
| id | doaj.art-619c1f312bb941fcb26e83afa53764bd |
| institution | Directory Open Access Journal |
| issn | 1445-5781 1447-0578 |
| language | English |
| last_indexed | 2024-12-10T15:33:42Z |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Reproductive Medicine and Biology |
| spelling | doaj.art-619c1f312bb941fcb26e83afa53764bd2022-12-22T01:43:18ZengWileyReproductive Medicine and Biology1445-57811447-05782019-10-0118435736110.1002/rmb2.12290Piezo‐assisted ICSI improves fertilization and blastocyst development rates compared with conventional ICSI in women aged more than 35 yearsKohyu Furuhashi0Yoshimi Saeki1Noritoshi Enatsu2Toshiroh Iwasaki3Koichi Ito4Yuri Mizusawa5Yukiko Matsumoto6Shoji Kokeguchi7Masahide Shiotani8Hanabusa Women’s Clinic Kobe Hyogo JapanHanabusa Women’s Clinic Kobe Hyogo JapanHanabusa Women’s Clinic Kobe Hyogo JapanHanabusa Women’s Clinic Kobe Hyogo JapanHanabusa Women’s Clinic Kobe Hyogo JapanHanabusa Women’s Clinic Kobe Hyogo JapanHanabusa Women’s Clinic Kobe Hyogo JapanHanabusa Women’s Clinic Kobe Hyogo JapanHanabusa Women’s Clinic Kobe Hyogo JapanAbstract Purpose Piezo‐assisted intracytoplasmic sperm injection (Piezo‐ICSI) is reported to be an effective method for inseminating fragile oocytes compared with conventional ICSI (c‐ICSI). However, infertile patient groups suitable for Piezo‐ICSI have not been elucidated. This study was conducted to determine age groups suitable for Piezo‐ICSI using sibling egg controls inseminated by a well‐trained embryologist to reduce technical inequalities. Methods A total of 947 matured oocytes were inseminated either by Piezo‐ICSI or by c‐ICSI in sibling oocytes as controls. Fertilization (2 pronuclei, PN), survival, and blastocyst development rates on day (D) 5 and D6 after insemination were compared between the Piezo‐ICSI and c‐ICSI groups. Further analyses were applied to groups of women >35 or ≤35 years of age. Results There were no significant differences in fertilization, survival, or blastocyst development rates between the two insemination treatment groups. However, for women >35 years of age, the fertilization (P = .008) and blastocyst development (P = .016) rates with Piezo‐ICSI on D5 and D6 were significantly higher than in those subjected to c‐ICSI. Conclusions Piezo‐ICSI was useful for inseminating oocytes from women >35 years of age.https://doi.org/10.1002/rmb2.12290agingblastocyst development ratefertilization rateintracytoplasmic sperm injectionPiezo‐ICSI |
| spellingShingle | Kohyu Furuhashi Yoshimi Saeki Noritoshi Enatsu Toshiroh Iwasaki Koichi Ito Yuri Mizusawa Yukiko Matsumoto Shoji Kokeguchi Masahide Shiotani Piezo‐assisted ICSI improves fertilization and blastocyst development rates compared with conventional ICSI in women aged more than 35 years Reproductive Medicine and Biology aging blastocyst development rate fertilization rate intracytoplasmic sperm injection Piezo‐ICSI |
| title | Piezo‐assisted ICSI improves fertilization and blastocyst development rates compared with conventional ICSI in women aged more than 35 years |
| title_full | Piezo‐assisted ICSI improves fertilization and blastocyst development rates compared with conventional ICSI in women aged more than 35 years |
| title_fullStr | Piezo‐assisted ICSI improves fertilization and blastocyst development rates compared with conventional ICSI in women aged more than 35 years |
| title_full_unstemmed | Piezo‐assisted ICSI improves fertilization and blastocyst development rates compared with conventional ICSI in women aged more than 35 years |
| title_short | Piezo‐assisted ICSI improves fertilization and blastocyst development rates compared with conventional ICSI in women aged more than 35 years |
| title_sort | piezo assisted icsi improves fertilization and blastocyst development rates compared with conventional icsi in women aged more than 35 years |
| topic | aging blastocyst development rate fertilization rate intracytoplasmic sperm injection Piezo‐ICSI |
| url | https://doi.org/10.1002/rmb2.12290 |
| work_keys_str_mv | AT kohyufuruhashi piezoassistedicsiimprovesfertilizationandblastocystdevelopmentratescomparedwithconventionalicsiinwomenagedmorethan35years AT yoshimisaeki piezoassistedicsiimprovesfertilizationandblastocystdevelopmentratescomparedwithconventionalicsiinwomenagedmorethan35years AT noritoshienatsu piezoassistedicsiimprovesfertilizationandblastocystdevelopmentratescomparedwithconventionalicsiinwomenagedmorethan35years AT toshirohiwasaki piezoassistedicsiimprovesfertilizationandblastocystdevelopmentratescomparedwithconventionalicsiinwomenagedmorethan35years AT koichiito piezoassistedicsiimprovesfertilizationandblastocystdevelopmentratescomparedwithconventionalicsiinwomenagedmorethan35years AT yurimizusawa piezoassistedicsiimprovesfertilizationandblastocystdevelopmentratescomparedwithconventionalicsiinwomenagedmorethan35years AT yukikomatsumoto piezoassistedicsiimprovesfertilizationandblastocystdevelopmentratescomparedwithconventionalicsiinwomenagedmorethan35years AT shojikokeguchi piezoassistedicsiimprovesfertilizationandblastocystdevelopmentratescomparedwithconventionalicsiinwomenagedmorethan35years AT masahideshiotani piezoassistedicsiimprovesfertilizationandblastocystdevelopmentratescomparedwithconventionalicsiinwomenagedmorethan35years |