Anti-Inflammatory Effects of Miyako <i>Bidens pilosa</i> in a Mouse Model of Amyotrophic Lateral Sclerosis and Lipopolysaccharide-Stimulated BV-2 Microglia
Neuroinflammation is a fundamental feature in the pathogenesis of amyotrophic lateral sclerosis (ALS) and arises from the activation of astrocytes and microglial cells. Previously, we reported that Miyako <i>Bidens pilosa</i> extract (MBP) inhibited microglial activation and prolonged th...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/13698 |
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| author | Komugi Tsuruta Takato Shidara Hiroko Miyagishi Hiroshi Nango Yoshihiko Nakatani Naoto Suzuki Taku Amano Toyofumi Suzuki Yasuhiro Kosuge |
| author_facet | Komugi Tsuruta Takato Shidara Hiroko Miyagishi Hiroshi Nango Yoshihiko Nakatani Naoto Suzuki Taku Amano Toyofumi Suzuki Yasuhiro Kosuge |
| author_sort | Komugi Tsuruta |
| collection | DOAJ |
| description | Neuroinflammation is a fundamental feature in the pathogenesis of amyotrophic lateral sclerosis (ALS) and arises from the activation of astrocytes and microglial cells. Previously, we reported that Miyako <i>Bidens pilosa</i> extract (MBP) inhibited microglial activation and prolonged the life span in a human ALS-linked mutant <i>superoxide dismutase-1</i> (<i>SOD1</i><sup>G93A</sup>) transgenic mouse model of ALS (G93A mice). Herein, we evaluated the effect of MBP on microglial activation in the spinal cord of G93A mice and lipopolysaccharide-stimulated BV-2 microglial cells. The administration of MBP inhibited the upregulation of the M1-microglia/macrophage marker (interferon-γ receptor (IFN-γR)) and pro-inflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6) in G93A mice. However, MBP did not affect the increase in the M2-microglia/macrophage marker (IL-13R) and anti-inflammatory cytokines (transforming growth factor (TGF)-β and IL-10) in G93A mice. BV-2 cell exposure to MBP resulted in a decrease in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) reduction activity and bromodeoxyuridine incorporation, without an increase in the number of ethidium homodimer-1-stained dead cells. Moreover, MBP suppressed the production of lipopolysaccharide-induced pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) in BV-2 cells. These results suggest that the selective suppression of M1-related pro-inflammatory cytokines is involved in the therapeutic potential of MBP in ALS model mice. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:41:11Z |
| publishDate | 2023-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-619d5bbdb66843daadb5a00d77cbbabd2023-11-19T11:01:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181369810.3390/ijms241813698Anti-Inflammatory Effects of Miyako <i>Bidens pilosa</i> in a Mouse Model of Amyotrophic Lateral Sclerosis and Lipopolysaccharide-Stimulated BV-2 MicrogliaKomugi Tsuruta0Takato Shidara1Hiroko Miyagishi2Hiroshi Nango3Yoshihiko Nakatani4Naoto Suzuki5Taku Amano6Toyofumi Suzuki7Yasuhiro Kosuge8Laboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi 274-8555, Chiba, JapanLaboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi 274-8555, Chiba, JapanLaboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi 274-8555, Chiba, JapanLaboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi 274-8555, Chiba, JapanDepartment of Pharmacotherapeutics, School of Pharmacy, International University of Health and Welfare, 2600-1 Kitakanemaru, Ohtawara 324-8501, Tochigi, JapanLaboratory of Pharmaceutics, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi 274-8555, Chiba, JapanTochigi Prefectural Okamotodai Hospital, 2162 Shimookamotomachi, Utsunomiya 329-1104, Tochigi, JapanLaboratory of Pharmaceutics, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi 274-8555, Chiba, JapanLaboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi 274-8555, Chiba, JapanNeuroinflammation is a fundamental feature in the pathogenesis of amyotrophic lateral sclerosis (ALS) and arises from the activation of astrocytes and microglial cells. Previously, we reported that Miyako <i>Bidens pilosa</i> extract (MBP) inhibited microglial activation and prolonged the life span in a human ALS-linked mutant <i>superoxide dismutase-1</i> (<i>SOD1</i><sup>G93A</sup>) transgenic mouse model of ALS (G93A mice). Herein, we evaluated the effect of MBP on microglial activation in the spinal cord of G93A mice and lipopolysaccharide-stimulated BV-2 microglial cells. The administration of MBP inhibited the upregulation of the M1-microglia/macrophage marker (interferon-γ receptor (IFN-γR)) and pro-inflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6) in G93A mice. However, MBP did not affect the increase in the M2-microglia/macrophage marker (IL-13R) and anti-inflammatory cytokines (transforming growth factor (TGF)-β and IL-10) in G93A mice. BV-2 cell exposure to MBP resulted in a decrease in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) reduction activity and bromodeoxyuridine incorporation, without an increase in the number of ethidium homodimer-1-stained dead cells. Moreover, MBP suppressed the production of lipopolysaccharide-induced pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) in BV-2 cells. These results suggest that the selective suppression of M1-related pro-inflammatory cytokines is involved in the therapeutic potential of MBP in ALS model mice.https://www.mdpi.com/1422-0067/24/18/13698amyotrophic lateral sclerosis<i>Bidens pilosa</i>pro-inflammatory cytokinesspinal cordM1-microglia/macrophages |
| spellingShingle | Komugi Tsuruta Takato Shidara Hiroko Miyagishi Hiroshi Nango Yoshihiko Nakatani Naoto Suzuki Taku Amano Toyofumi Suzuki Yasuhiro Kosuge Anti-Inflammatory Effects of Miyako <i>Bidens pilosa</i> in a Mouse Model of Amyotrophic Lateral Sclerosis and Lipopolysaccharide-Stimulated BV-2 Microglia International Journal of Molecular Sciences amyotrophic lateral sclerosis <i>Bidens pilosa</i> pro-inflammatory cytokines spinal cord M1-microglia/macrophages |
| title | Anti-Inflammatory Effects of Miyako <i>Bidens pilosa</i> in a Mouse Model of Amyotrophic Lateral Sclerosis and Lipopolysaccharide-Stimulated BV-2 Microglia |
| title_full | Anti-Inflammatory Effects of Miyako <i>Bidens pilosa</i> in a Mouse Model of Amyotrophic Lateral Sclerosis and Lipopolysaccharide-Stimulated BV-2 Microglia |
| title_fullStr | Anti-Inflammatory Effects of Miyako <i>Bidens pilosa</i> in a Mouse Model of Amyotrophic Lateral Sclerosis and Lipopolysaccharide-Stimulated BV-2 Microglia |
| title_full_unstemmed | Anti-Inflammatory Effects of Miyako <i>Bidens pilosa</i> in a Mouse Model of Amyotrophic Lateral Sclerosis and Lipopolysaccharide-Stimulated BV-2 Microglia |
| title_short | Anti-Inflammatory Effects of Miyako <i>Bidens pilosa</i> in a Mouse Model of Amyotrophic Lateral Sclerosis and Lipopolysaccharide-Stimulated BV-2 Microglia |
| title_sort | anti inflammatory effects of miyako i bidens pilosa i in a mouse model of amyotrophic lateral sclerosis and lipopolysaccharide stimulated bv 2 microglia |
| topic | amyotrophic lateral sclerosis <i>Bidens pilosa</i> pro-inflammatory cytokines spinal cord M1-microglia/macrophages |
| url | https://www.mdpi.com/1422-0067/24/18/13698 |
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