Cell Cycle Regulation by NF-YC in <i>Drosophila</i> Eye Imaginal Disc: Implications for Synchronization in the Non-Proliferative Region
Cell cycle progression during development is meticulously coordinated with differentiation. This is particularly evident in the <i>Drosophila</i> 3rd instar eye imaginal disc, where the cell cycle is synchronized and arrests at the G1 phase in the non-proliferative region (NPR), setting...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/15/12203 |
| _version_ | 1797586672809934848 |
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| author | Anthony Avellino Chen-Huan Peng Ming-Der Lin |
| author_facet | Anthony Avellino Chen-Huan Peng Ming-Der Lin |
| author_sort | Anthony Avellino |
| collection | DOAJ |
| description | Cell cycle progression during development is meticulously coordinated with differentiation. This is particularly evident in the <i>Drosophila</i> 3rd instar eye imaginal disc, where the cell cycle is synchronized and arrests at the G1 phase in the non-proliferative region (NPR), setting the stage for photoreceptor cell differentiation. Here, we identify the transcription factor Nuclear Factor-YC (NF-YC) as a crucial player in this finely tuned progression, elucidating its specific role in the synchronized movement of the morphogenetic furrow. Depletion of <i>NF-YC</i> leads to extended expression of Cyclin A (CycA) and Cyclin B (CycB) from the FMW to the NPR. Notably, <i>NF-YC</i> knockdown resulted in decreased expression of Eyes absent (Eya) but did not affect Decapentaplegic (Dpp) and Hedgehog (Hh). Our findings highlight the role of NF-YC in restricting the expression of CycA and CycB in the NPR, thereby facilitating cell-cycle synchronization. Moreover, we identify the transcriptional cofactor Eya as a downstream target of NF-YC, revealing a new regulatory pathway in <i>Drosophila</i> eye development. This study expands our understanding of NF-YC’s role from cell cycle control to encompass developmental processes. |
| first_indexed | 2024-03-11T00:25:25Z |
| format | Article |
| id | doaj.art-619e4902ee5240f7afab834b375e2ed8 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T00:25:25Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-619e4902ee5240f7afab834b375e2ed82023-11-18T23:01:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124151220310.3390/ijms241512203Cell Cycle Regulation by NF-YC in <i>Drosophila</i> Eye Imaginal Disc: Implications for Synchronization in the Non-Proliferative RegionAnthony Avellino0Chen-Huan Peng1Ming-Der Lin2Department of Molecular Biology and Human Genetics, Tzu Chi University, 701 Zhongyang Rd., Sec. 3, Hualien 97004, TaiwanDepartment of Orthopedics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, 707 Zhongyang Rd., Sec. 3, Hualien 97002, TaiwanDepartment of Molecular Biology and Human Genetics, Tzu Chi University, 701 Zhongyang Rd., Sec. 3, Hualien 97004, TaiwanCell cycle progression during development is meticulously coordinated with differentiation. This is particularly evident in the <i>Drosophila</i> 3rd instar eye imaginal disc, where the cell cycle is synchronized and arrests at the G1 phase in the non-proliferative region (NPR), setting the stage for photoreceptor cell differentiation. Here, we identify the transcription factor Nuclear Factor-YC (NF-YC) as a crucial player in this finely tuned progression, elucidating its specific role in the synchronized movement of the morphogenetic furrow. Depletion of <i>NF-YC</i> leads to extended expression of Cyclin A (CycA) and Cyclin B (CycB) from the FMW to the NPR. Notably, <i>NF-YC</i> knockdown resulted in decreased expression of Eyes absent (Eya) but did not affect Decapentaplegic (Dpp) and Hedgehog (Hh). Our findings highlight the role of NF-YC in restricting the expression of CycA and CycB in the NPR, thereby facilitating cell-cycle synchronization. Moreover, we identify the transcriptional cofactor Eya as a downstream target of NF-YC, revealing a new regulatory pathway in <i>Drosophila</i> eye development. This study expands our understanding of NF-YC’s role from cell cycle control to encompass developmental processes.https://www.mdpi.com/1422-0067/24/15/12203Nuclear Factor-YCeyes absentmorphogenetic furrowcell cycle synchronization |
| spellingShingle | Anthony Avellino Chen-Huan Peng Ming-Der Lin Cell Cycle Regulation by NF-YC in <i>Drosophila</i> Eye Imaginal Disc: Implications for Synchronization in the Non-Proliferative Region International Journal of Molecular Sciences Nuclear Factor-YC eyes absent morphogenetic furrow cell cycle synchronization |
| title | Cell Cycle Regulation by NF-YC in <i>Drosophila</i> Eye Imaginal Disc: Implications for Synchronization in the Non-Proliferative Region |
| title_full | Cell Cycle Regulation by NF-YC in <i>Drosophila</i> Eye Imaginal Disc: Implications for Synchronization in the Non-Proliferative Region |
| title_fullStr | Cell Cycle Regulation by NF-YC in <i>Drosophila</i> Eye Imaginal Disc: Implications for Synchronization in the Non-Proliferative Region |
| title_full_unstemmed | Cell Cycle Regulation by NF-YC in <i>Drosophila</i> Eye Imaginal Disc: Implications for Synchronization in the Non-Proliferative Region |
| title_short | Cell Cycle Regulation by NF-YC in <i>Drosophila</i> Eye Imaginal Disc: Implications for Synchronization in the Non-Proliferative Region |
| title_sort | cell cycle regulation by nf yc in i drosophila i eye imaginal disc implications for synchronization in the non proliferative region |
| topic | Nuclear Factor-YC eyes absent morphogenetic furrow cell cycle synchronization |
| url | https://www.mdpi.com/1422-0067/24/15/12203 |
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