Sex Differences of Cardiolipin in Tissue Distribution Based on Targeted Lipidomic Analysis by UHPLC-QTOF-MS/MS
Cardiolipins (CLs) are involved in ATP production, mitochondria biogenesis, apoptosis and mitophagy. Their tissue distribution can provide insight into the function of mitochondria and related diseases. However, the reports on tissue distribution of CLs remain limited. In this research, CLs were ide...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/20/6988 |
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| author | Yuqi Lin Xugui Li Mengxiang Dai Qiaoyu Li Qingxin Shi Lijun Zhang Rongzeng Huang Chengwu Song Shuna Jin |
| author_facet | Yuqi Lin Xugui Li Mengxiang Dai Qiaoyu Li Qingxin Shi Lijun Zhang Rongzeng Huang Chengwu Song Shuna Jin |
| author_sort | Yuqi Lin |
| collection | DOAJ |
| description | Cardiolipins (CLs) are involved in ATP production, mitochondria biogenesis, apoptosis and mitophagy. Their tissue distribution can provide insight into the function of mitochondria and related diseases. However, the reports on tissue distribution of CLs remain limited. In this research, CLs were identified from heart, liver, kidney, spleen, lung, skeletal muscle, and brain using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS). Then, the distribution and sex difference of CLs in seven tissues were compared by a targeted lipidomic approach. A total of 88 CLs were identified, of which 58, 51, 57, 58, 50, 61 and 52 CLs were found in heart, liver, kidney, spleen, lung, skeletal muscle, and brain, respectively. Compared with the distribution of CLs in heart, liver, kidney, and skeletal muscle, the CLs in spleen, lung, and brain showed significant differences. Moreover, the results indicated that there were sex differences of CLs in liver and kidney. A total of 16 CLs in liver tissue and 21 CLs in kidney tissue, with significant sex differences, were screened. Our findings in the targeted lipidomic analysis demonstrated that tissue distribution of CLs was essential in the dynamic states and sex differences of CLs, which might provide evidence for the mitochondrial-related mechanism under physiological and pathological conditions. |
| first_indexed | 2024-03-09T19:42:24Z |
| format | Article |
| id | doaj.art-61a02c17521c49548c648ba3246fad2b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T19:42:24Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-61a02c17521c49548c648ba3246fad2b2023-11-24T01:35:10ZengMDPI AGMolecules1420-30492022-10-012720698810.3390/molecules27206988Sex Differences of Cardiolipin in Tissue Distribution Based on Targeted Lipidomic Analysis by UHPLC-QTOF-MS/MSYuqi Lin0Xugui Li1Mengxiang Dai2Qiaoyu Li3Qingxin Shi4Lijun Zhang5Rongzeng Huang6Chengwu Song7Shuna Jin8College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, ChinaHubei 672 Orthopaedics Hospital of Integrated Chinese and Western Medicine, Wuhan 430079, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, ChinaCollege of Basic Medicine, Hubei University of Chinese Medicine, Wuhan 430065, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, ChinaCollege of Basic Medicine, Hubei University of Chinese Medicine, Wuhan 430065, ChinaCardiolipins (CLs) are involved in ATP production, mitochondria biogenesis, apoptosis and mitophagy. Their tissue distribution can provide insight into the function of mitochondria and related diseases. However, the reports on tissue distribution of CLs remain limited. In this research, CLs were identified from heart, liver, kidney, spleen, lung, skeletal muscle, and brain using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS). Then, the distribution and sex difference of CLs in seven tissues were compared by a targeted lipidomic approach. A total of 88 CLs were identified, of which 58, 51, 57, 58, 50, 61 and 52 CLs were found in heart, liver, kidney, spleen, lung, skeletal muscle, and brain, respectively. Compared with the distribution of CLs in heart, liver, kidney, and skeletal muscle, the CLs in spleen, lung, and brain showed significant differences. Moreover, the results indicated that there were sex differences of CLs in liver and kidney. A total of 16 CLs in liver tissue and 21 CLs in kidney tissue, with significant sex differences, were screened. Our findings in the targeted lipidomic analysis demonstrated that tissue distribution of CLs was essential in the dynamic states and sex differences of CLs, which might provide evidence for the mitochondrial-related mechanism under physiological and pathological conditions.https://www.mdpi.com/1420-3049/27/20/6988cardiolipinLC-MS/MStissue distributionsex differencetargeted lipidomicsmultivariate statistical analysis |
| spellingShingle | Yuqi Lin Xugui Li Mengxiang Dai Qiaoyu Li Qingxin Shi Lijun Zhang Rongzeng Huang Chengwu Song Shuna Jin Sex Differences of Cardiolipin in Tissue Distribution Based on Targeted Lipidomic Analysis by UHPLC-QTOF-MS/MS Molecules cardiolipin LC-MS/MS tissue distribution sex difference targeted lipidomics multivariate statistical analysis |
| title | Sex Differences of Cardiolipin in Tissue Distribution Based on Targeted Lipidomic Analysis by UHPLC-QTOF-MS/MS |
| title_full | Sex Differences of Cardiolipin in Tissue Distribution Based on Targeted Lipidomic Analysis by UHPLC-QTOF-MS/MS |
| title_fullStr | Sex Differences of Cardiolipin in Tissue Distribution Based on Targeted Lipidomic Analysis by UHPLC-QTOF-MS/MS |
| title_full_unstemmed | Sex Differences of Cardiolipin in Tissue Distribution Based on Targeted Lipidomic Analysis by UHPLC-QTOF-MS/MS |
| title_short | Sex Differences of Cardiolipin in Tissue Distribution Based on Targeted Lipidomic Analysis by UHPLC-QTOF-MS/MS |
| title_sort | sex differences of cardiolipin in tissue distribution based on targeted lipidomic analysis by uhplc qtof ms ms |
| topic | cardiolipin LC-MS/MS tissue distribution sex difference targeted lipidomics multivariate statistical analysis |
| url | https://www.mdpi.com/1420-3049/27/20/6988 |
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