Wide Grain 3, a GRAS Protein, Interacts with DLT to Regulate Grain Size and Brassinosteroid Signaling in Rice
Abstract Background: Grain size is a direct determinant of grain weight and yield in rice; however, the genetic and molecular mechanisms determining grain size remain largely unknown. Findings: We identified a mutant, wide grain 3 (wg3), which exhibited significantly increased grain width and 1000-g...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-11-01
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| Series: | Rice |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12284-022-00601-4 |
| _version_ | 1828094914605350912 |
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| author | Weilan Chen Xiaoling Hu Li Hu Xinyue Hou Zhengyan Xu Fanmin Yang Min Yuan Feifan Chen Yunxiao Wang Bin Tu Ting Li Liangzhu Kang Shiwen Tang Bingtian Ma Yuping Wang Shigui Li Peng Qin Hua Yuan |
| author_facet | Weilan Chen Xiaoling Hu Li Hu Xinyue Hou Zhengyan Xu Fanmin Yang Min Yuan Feifan Chen Yunxiao Wang Bin Tu Ting Li Liangzhu Kang Shiwen Tang Bingtian Ma Yuping Wang Shigui Li Peng Qin Hua Yuan |
| author_sort | Weilan Chen |
| collection | DOAJ |
| description | Abstract Background: Grain size is a direct determinant of grain weight and yield in rice; however, the genetic and molecular mechanisms determining grain size remain largely unknown. Findings: We identified a mutant, wide grain 3 (wg3), which exhibited significantly increased grain width and 1000-grain weight. Cytological analysis showed that WG3 regulates grain size by affecting cell proliferation. MutMap-based gene cloning and a transgenic experiment demonstrated that WG3 encodes a GRAS protein. Moreover, we found that WG3 directly interacts with DWARF AND LOW-TILLERING (DLT), a previously reported GRAS protein, and a genetic experiment demonstrated that WG3 and DLT function in a common pathway to regulate grain size. Additionally, a brassinosteroid (BR) sensitivity test suggested that WG3 has a positive role in BR signaling in rice. Collectively, our results reveal a new genetic and molecular mechanism for the regulation of grain size in rice by the WG3-DLT complex, and highlight the important functions of the GRAS protein complex in plants. Conclusion: WG3 functions directly in regulating grain size and BR signaling in rice. |
| first_indexed | 2024-04-11T07:05:40Z |
| format | Article |
| id | doaj.art-61a1b45fea7d441db9fbb4a34e529e10 |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-04-11T07:05:40Z |
| publishDate | 2022-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-61a1b45fea7d441db9fbb4a34e529e102022-12-22T04:38:25ZengSpringerOpenRice1939-84251939-84332022-11-011511610.1186/s12284-022-00601-4Wide Grain 3, a GRAS Protein, Interacts with DLT to Regulate Grain Size and Brassinosteroid Signaling in RiceWeilan Chen0Xiaoling Hu1Li Hu2Xinyue Hou3Zhengyan Xu4Fanmin Yang5Min Yuan6Feifan Chen7Yunxiao Wang8Bin Tu9Ting Li10Liangzhu Kang11Shiwen Tang12Bingtian Ma13Yuping Wang14Shigui Li15Peng Qin16Hua Yuan17State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityCollege of Agriculture, Forestry and Health, The Open University of SichuanState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural UniversityAbstract Background: Grain size is a direct determinant of grain weight and yield in rice; however, the genetic and molecular mechanisms determining grain size remain largely unknown. Findings: We identified a mutant, wide grain 3 (wg3), which exhibited significantly increased grain width and 1000-grain weight. Cytological analysis showed that WG3 regulates grain size by affecting cell proliferation. MutMap-based gene cloning and a transgenic experiment demonstrated that WG3 encodes a GRAS protein. Moreover, we found that WG3 directly interacts with DWARF AND LOW-TILLERING (DLT), a previously reported GRAS protein, and a genetic experiment demonstrated that WG3 and DLT function in a common pathway to regulate grain size. Additionally, a brassinosteroid (BR) sensitivity test suggested that WG3 has a positive role in BR signaling in rice. Collectively, our results reveal a new genetic and molecular mechanism for the regulation of grain size in rice by the WG3-DLT complex, and highlight the important functions of the GRAS protein complex in plants. Conclusion: WG3 functions directly in regulating grain size and BR signaling in rice.https://doi.org/10.1186/s12284-022-00601-4RiceGrain sizeWG3DLTGRASBrassinosteroid signaling |
| spellingShingle | Weilan Chen Xiaoling Hu Li Hu Xinyue Hou Zhengyan Xu Fanmin Yang Min Yuan Feifan Chen Yunxiao Wang Bin Tu Ting Li Liangzhu Kang Shiwen Tang Bingtian Ma Yuping Wang Shigui Li Peng Qin Hua Yuan Wide Grain 3, a GRAS Protein, Interacts with DLT to Regulate Grain Size and Brassinosteroid Signaling in Rice Rice Rice Grain size WG3 DLT GRAS Brassinosteroid signaling |
| title | Wide Grain 3, a GRAS Protein, Interacts with DLT to Regulate Grain Size and Brassinosteroid Signaling in Rice |
| title_full | Wide Grain 3, a GRAS Protein, Interacts with DLT to Regulate Grain Size and Brassinosteroid Signaling in Rice |
| title_fullStr | Wide Grain 3, a GRAS Protein, Interacts with DLT to Regulate Grain Size and Brassinosteroid Signaling in Rice |
| title_full_unstemmed | Wide Grain 3, a GRAS Protein, Interacts with DLT to Regulate Grain Size and Brassinosteroid Signaling in Rice |
| title_short | Wide Grain 3, a GRAS Protein, Interacts with DLT to Regulate Grain Size and Brassinosteroid Signaling in Rice |
| title_sort | wide grain 3 a gras protein interacts with dlt to regulate grain size and brassinosteroid signaling in rice |
| topic | Rice Grain size WG3 DLT GRAS Brassinosteroid signaling |
| url | https://doi.org/10.1186/s12284-022-00601-4 |
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