Extracellular biosynthesis of silver nanoparticles using Bacillus subtilis and their antibacterial activity against clinical bacterial species
The aims of this study were to biosynthesize silver nanoparticles (AgNPs) using Bacillus subtilis supernatant, and to evaluate their in vitro antibacterial potential against human pathogens; namely Staphylococcus aureus (Staph. aureus) and Escherichia coli (E. coli). Nanoparticles (NPs) are becom...
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| Format: | Article |
| Language: | English |
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The National Information and Documentation Centre (NIDOC) , Egypt
2021-06-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_178303_ccd61348cfb660e7f2fa5d32451ef8b8.pdf |
| _version_ | 1797203298231517184 |
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| author | Nouran H. Assar Aya allah T. Mohamed Rehab M. Abd El-Baky Reham Ali Ibrahem |
| author_facet | Nouran H. Assar Aya allah T. Mohamed Rehab M. Abd El-Baky Reham Ali Ibrahem |
| author_sort | Nouran H. Assar |
| collection | DOAJ |
| description | The aims of this study were to biosynthesize silver nanoparticles (AgNPs) using Bacillus
subtilis supernatant, and to evaluate their in vitro antibacterial potential against human
pathogens; namely Staphylococcus aureus (Staph. aureus) and Escherichia coli (E. coli).
Nanoparticles (NPs) are becoming popular in different fields of research, and are useful in
combating vast number of microbial diseases. NPs may be artificially synthesized in vitro
using chemical methods and\or via extracellular metabolites produced by the bacterial strains.
In the present study, biosynthesis of AgNPs was carried out in vitro using supernatants of B.
subtilis. Biosynthesized AgNPs were characterized through several physical methods. The
recorded Z-average (d. nm) was 135.0 nm; with 99.2 % of the NPs displaying a hydrodynamic
distance across of 188.0 nm (SD= 117.7). The polydispersity index was 0.246 and the Zetapotential value was - 17.2 mV, which indicates good colloidal stability. Results of the
Transmission electron microscope (TEM) observation indicated that the particles were
spherical in shape with an average size of 21.8- 27.5 nm. The antibacterial efficacy of the
AgNPs against Methicillin resistant Staph. aureus (MRSA) and E. coli clinical isolates was
evaluated in vitro using the agar well diffusion. The AgNPs demonstrated antibacterial
potential against MRSA and E. coli isolates; recording 18 and 15 mm diameter of zones of
inhibition, respectively. The minimum inhibitory concentration (MIC) was found to be 142
µg/ ml, while the recorded minimum bactericidal concentration (MBC) was 284 µg/ ml. The
mode of action of the AgNPs was investigated using the Scanning electron microscope (SEM),
which was recognized as bacterial cell lysis and elongation. Current data suggest an efficient
biosynthesis of stable AgNPs by B. subtilis with remarkable antibacterial potential. |
| first_indexed | 2024-04-24T08:17:06Z |
| format | Article |
| id | doaj.art-61a2a57778454214bab8f860c8db7218 |
| institution | Directory Open Access Journal |
| issn | 2537-0286 2537-0294 |
| language | English |
| last_indexed | 2024-04-24T08:17:06Z |
| publishDate | 2021-06-01 |
| publisher | The National Information and Documentation Centre (NIDOC) , Egypt |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj.art-61a2a57778454214bab8f860c8db72182024-04-17T03:19:32ZengThe National Information and Documentation Centre (NIDOC) , EgyptNovel Research in Microbiology Journal2537-02862537-02942021-06-01531256126810.21608/nrmj.2021.178303Extracellular biosynthesis of silver nanoparticles using Bacillus subtilis and their antibacterial activity against clinical bacterial speciesNouran H. Assar0Aya allah T. Mohamed1Rehab M. Abd El-Baky2Reham Ali Ibrahem3Department of Microbiology, General Division of Basic Medical Sciences, Egyptian Drug Authority former National Organization for Drug Control and Research (NODCAR), Giza, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia, Egypt; Microbiology and Immunology Department, Faculty of Pharmacy, Deraya University, Minia, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia, Egypt; Microbiology and Immunology Department, Faculty of Pharmacy, Deraya University, Minia, EgyptMicrobiology and Immunology Department, Faculty of Pharmacy, Deraya University, Minia, EgyptThe aims of this study were to biosynthesize silver nanoparticles (AgNPs) using Bacillus subtilis supernatant, and to evaluate their in vitro antibacterial potential against human pathogens; namely Staphylococcus aureus (Staph. aureus) and Escherichia coli (E. coli). Nanoparticles (NPs) are becoming popular in different fields of research, and are useful in combating vast number of microbial diseases. NPs may be artificially synthesized in vitro using chemical methods and\or via extracellular metabolites produced by the bacterial strains. In the present study, biosynthesis of AgNPs was carried out in vitro using supernatants of B. subtilis. Biosynthesized AgNPs were characterized through several physical methods. The recorded Z-average (d. nm) was 135.0 nm; with 99.2 % of the NPs displaying a hydrodynamic distance across of 188.0 nm (SD= 117.7). The polydispersity index was 0.246 and the Zetapotential value was - 17.2 mV, which indicates good colloidal stability. Results of the Transmission electron microscope (TEM) observation indicated that the particles were spherical in shape with an average size of 21.8- 27.5 nm. The antibacterial efficacy of the AgNPs against Methicillin resistant Staph. aureus (MRSA) and E. coli clinical isolates was evaluated in vitro using the agar well diffusion. The AgNPs demonstrated antibacterial potential against MRSA and E. coli isolates; recording 18 and 15 mm diameter of zones of inhibition, respectively. The minimum inhibitory concentration (MIC) was found to be 142 µg/ ml, while the recorded minimum bactericidal concentration (MBC) was 284 µg/ ml. The mode of action of the AgNPs was investigated using the Scanning electron microscope (SEM), which was recognized as bacterial cell lysis and elongation. Current data suggest an efficient biosynthesis of stable AgNPs by B. subtilis with remarkable antibacterial potential.https://nrmj.journals.ekb.eg/article_178303_ccd61348cfb660e7f2fa5d32451ef8b8.pdfbacillus subtilisbiosynthesissilver nanoparticlesmrsae. coli |
| spellingShingle | Nouran H. Assar Aya allah T. Mohamed Rehab M. Abd El-Baky Reham Ali Ibrahem Extracellular biosynthesis of silver nanoparticles using Bacillus subtilis and their antibacterial activity against clinical bacterial species Novel Research in Microbiology Journal bacillus subtilis biosynthesis silver nanoparticles mrsa e. coli |
| title | Extracellular biosynthesis of silver nanoparticles using Bacillus subtilis and their antibacterial activity against clinical bacterial species |
| title_full | Extracellular biosynthesis of silver nanoparticles using Bacillus subtilis and their antibacterial activity against clinical bacterial species |
| title_fullStr | Extracellular biosynthesis of silver nanoparticles using Bacillus subtilis and their antibacterial activity against clinical bacterial species |
| title_full_unstemmed | Extracellular biosynthesis of silver nanoparticles using Bacillus subtilis and their antibacterial activity against clinical bacterial species |
| title_short | Extracellular biosynthesis of silver nanoparticles using Bacillus subtilis and their antibacterial activity against clinical bacterial species |
| title_sort | extracellular biosynthesis of silver nanoparticles using bacillus subtilis and their antibacterial activity against clinical bacterial species |
| topic | bacillus subtilis biosynthesis silver nanoparticles mrsa e. coli |
| url | https://nrmj.journals.ekb.eg/article_178303_ccd61348cfb660e7f2fa5d32451ef8b8.pdf |
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