Technique for High-Quality Protein Crystal Growth by Control of Subgrain Formation under an External Electric Field
X-ray diffraction (XRD) rocking-curves were measured for tetragonal hen egg white (HEW) lysozyme crystals grown with and without application of an external electric field, and the crystal quality was assessed according to the full width at half-maximums (FWHMs) of each rocking-curve profile. The ave...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-08-01
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| Series: | Crystals |
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| Online Access: | http://www.mdpi.com/2073-4352/6/8/95 |
| _version_ | 1798006804325597184 |
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| author | Haruhiko Koizumi Satoshi Uda Kozo Fujiwara Masaru Tachibana Kenichi Kojima Jun Nozawa |
| author_facet | Haruhiko Koizumi Satoshi Uda Kozo Fujiwara Masaru Tachibana Kenichi Kojima Jun Nozawa |
| author_sort | Haruhiko Koizumi |
| collection | DOAJ |
| description | X-ray diffraction (XRD) rocking-curves were measured for tetragonal hen egg white (HEW) lysozyme crystals grown with and without application of an external electric field, and the crystal quality was assessed according to the full width at half-maximums (FWHMs) of each rocking-curve profile. The average FWHMs for tetragonal HEW lysozyme crystals grown with an external electric field at 1 MHz were smaller than those for crystals grown without, especially for the 12 12 0 reflection. The crystal homogeneity of the tetragonal HEW lysozyme crystals was also improved under application of an external electric field at 1 MHz, compared to that without. Improvement of the crystal quality of tetragonal HEW lysozyme crystals grown under an applied field is discussed with a focus on subgrain formation. In addition, the origin of subgrain misorientation is also discussed with respect to the incorporation of impurities into protein crystals. |
| first_indexed | 2024-04-11T13:00:24Z |
| format | Article |
| id | doaj.art-61a3a6a9e12c481694292e2f4304062c |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-04-11T13:00:24Z |
| publishDate | 2016-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-61a3a6a9e12c481694292e2f4304062c2022-12-22T04:22:57ZengMDPI AGCrystals2073-43522016-08-01689510.3390/cryst6080095cryst6080095Technique for High-Quality Protein Crystal Growth by Control of Subgrain Formation under an External Electric FieldHaruhiko Koizumi0Satoshi Uda1Kozo Fujiwara2Masaru Tachibana3Kenichi Kojima4Jun Nozawa5Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanInstitute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanInstitute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanGraduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, JapanDepartment of Education, Yokohama Soei University, 1 Miho-tyou, Midori-ku, Yokohama 226-0015, JapanInstitute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, JapanX-ray diffraction (XRD) rocking-curves were measured for tetragonal hen egg white (HEW) lysozyme crystals grown with and without application of an external electric field, and the crystal quality was assessed according to the full width at half-maximums (FWHMs) of each rocking-curve profile. The average FWHMs for tetragonal HEW lysozyme crystals grown with an external electric field at 1 MHz were smaller than those for crystals grown without, especially for the 12 12 0 reflection. The crystal homogeneity of the tetragonal HEW lysozyme crystals was also improved under application of an external electric field at 1 MHz, compared to that without. Improvement of the crystal quality of tetragonal HEW lysozyme crystals grown under an applied field is discussed with a focus on subgrain formation. In addition, the origin of subgrain misorientation is also discussed with respect to the incorporation of impurities into protein crystals.http://www.mdpi.com/2073-4352/6/8/95protein crystalscrystal qualityelectric fieldrocking curve |
| spellingShingle | Haruhiko Koizumi Satoshi Uda Kozo Fujiwara Masaru Tachibana Kenichi Kojima Jun Nozawa Technique for High-Quality Protein Crystal Growth by Control of Subgrain Formation under an External Electric Field Crystals protein crystals crystal quality electric field rocking curve |
| title | Technique for High-Quality Protein Crystal Growth by Control of Subgrain Formation under an External Electric Field |
| title_full | Technique for High-Quality Protein Crystal Growth by Control of Subgrain Formation under an External Electric Field |
| title_fullStr | Technique for High-Quality Protein Crystal Growth by Control of Subgrain Formation under an External Electric Field |
| title_full_unstemmed | Technique for High-Quality Protein Crystal Growth by Control of Subgrain Formation under an External Electric Field |
| title_short | Technique for High-Quality Protein Crystal Growth by Control of Subgrain Formation under an External Electric Field |
| title_sort | technique for high quality protein crystal growth by control of subgrain formation under an external electric field |
| topic | protein crystals crystal quality electric field rocking curve |
| url | http://www.mdpi.com/2073-4352/6/8/95 |
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