CURRENT METHODS USED TO IDENTIFY AND GENOTYPE SPIROCHAETES BORRELIELLA BURGDORFERI
Lyme disease, as one of tick-borne diseases, has been a current epidemiological problem in Poland and in the world for several years. The proportion of borreliosis infections caused by B. burgdorferi spirochaetes is increasing. Difficulties diagnosing this disease with conventional methods have led...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2020-04-01
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| Series: | Health Problems of Civilization |
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| Online Access: | https://www.termedia.pl/CURRENT-METHODS-USED-TO-IDENTIFY-AND-GENOTYPE-SPIROCHAETES-BORRELIELLA-BURGDORFERI,99,40142,1,1.html |
| _version_ | 1819000535026499584 |
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| author | Patrycja Teodorowicz Marcin Weiner |
| author_facet | Patrycja Teodorowicz Marcin Weiner |
| author_sort | Patrycja Teodorowicz |
| collection | DOAJ |
| description | Lyme disease, as one of tick-borne diseases, has been a current epidemiological problem in Poland and in the world for several years. The proportion of borreliosis infections caused by B. burgdorferi spirochaetes is increasing. Difficulties diagnosing this disease with conventional methods have led to growing molecular biology research aimed at developing improved diagnostic tools. Molecular biology methods include all techniques that allow the detection and analysis of nucleic acids. Among them there are methods based on PCR reaction, molecular typing methods (MLST), new generation sequencing (NGS). The current development of this field gives great research opportunities. With regard to B. burgdorferi spirochete, molecular biology is used to: confirm Lyme borreliosis, identify and type Borreliella spirochetes, detect them in tick vectors or intermediate hosts, as well as to identify co-infections between different Borreliella species and other tick-borne pathogens. They are meant to significantly improve diagnostic analyzes. This paper reviews the current methods used for the detection and identification of B. burgdorferi. Molecular mechanisms for the survival of spirochetes in the host, infection vectors and clinical picture of Lyme disease were also discussed. |
| first_indexed | 2024-12-20T22:34:51Z |
| format | Article |
| id | doaj.art-61a9599f161445989849fb95788532d1 |
| institution | Directory Open Access Journal |
| issn | 2353-6942 2354-0265 |
| language | English |
| last_indexed | 2024-12-20T22:34:51Z |
| publishDate | 2020-04-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Health Problems of Civilization |
| spelling | doaj.art-61a9599f161445989849fb95788532d12022-12-21T19:24:38ZengTermedia Publishing HouseHealth Problems of Civilization2353-69422354-02652020-04-01142718210.5114/hpc.2020.9380840142CURRENT METHODS USED TO IDENTIFY AND GENOTYPE SPIROCHAETES BORRELIELLA BURGDORFERIPatrycja TeodorowiczMarcin WeinerLyme disease, as one of tick-borne diseases, has been a current epidemiological problem in Poland and in the world for several years. The proportion of borreliosis infections caused by B. burgdorferi spirochaetes is increasing. Difficulties diagnosing this disease with conventional methods have led to growing molecular biology research aimed at developing improved diagnostic tools. Molecular biology methods include all techniques that allow the detection and analysis of nucleic acids. Among them there are methods based on PCR reaction, molecular typing methods (MLST), new generation sequencing (NGS). The current development of this field gives great research opportunities. With regard to B. burgdorferi spirochete, molecular biology is used to: confirm Lyme borreliosis, identify and type Borreliella spirochetes, detect them in tick vectors or intermediate hosts, as well as to identify co-infections between different Borreliella species and other tick-borne pathogens. They are meant to significantly improve diagnostic analyzes. This paper reviews the current methods used for the detection and identification of B. burgdorferi. Molecular mechanisms for the survival of spirochetes in the host, infection vectors and clinical picture of Lyme disease were also discussed.https://www.termedia.pl/CURRENT-METHODS-USED-TO-IDENTIFY-AND-GENOTYPE-SPIROCHAETES-BORRELIELLA-BURGDORFERI,99,40142,1,1.htmldna pcr rna borreliella burgdorferi genospecies |
| spellingShingle | Patrycja Teodorowicz Marcin Weiner CURRENT METHODS USED TO IDENTIFY AND GENOTYPE SPIROCHAETES BORRELIELLA BURGDORFERI Health Problems of Civilization dna pcr rna borreliella burgdorferi genospecies |
| title | CURRENT METHODS USED TO IDENTIFY AND GENOTYPE SPIROCHAETES BORRELIELLA BURGDORFERI |
| title_full | CURRENT METHODS USED TO IDENTIFY AND GENOTYPE SPIROCHAETES BORRELIELLA BURGDORFERI |
| title_fullStr | CURRENT METHODS USED TO IDENTIFY AND GENOTYPE SPIROCHAETES BORRELIELLA BURGDORFERI |
| title_full_unstemmed | CURRENT METHODS USED TO IDENTIFY AND GENOTYPE SPIROCHAETES BORRELIELLA BURGDORFERI |
| title_short | CURRENT METHODS USED TO IDENTIFY AND GENOTYPE SPIROCHAETES BORRELIELLA BURGDORFERI |
| title_sort | current methods used to identify and genotype spirochaetes borreliella burgdorferi |
| topic | dna pcr rna borreliella burgdorferi genospecies |
| url | https://www.termedia.pl/CURRENT-METHODS-USED-TO-IDENTIFY-AND-GENOTYPE-SPIROCHAETES-BORRELIELLA-BURGDORFERI,99,40142,1,1.html |
| work_keys_str_mv | AT patrycjateodorowicz currentmethodsusedtoidentifyandgenotypespirochaetesborreliellaburgdorferi AT marcinweiner currentmethodsusedtoidentifyandgenotypespirochaetesborreliellaburgdorferi |