The influence of cobalt ions on growth and enzymatic activity of entomopathogenic fungi used in biological plant protection
This study focused on the effect of heavy metal cobalt ions (at concentrations of 1–1000 ppm) on the development and enzymatic activity of four entomopathogenic fungi: Beauveria bassiana, Beauveria brongniartii, Isaria fumosorosea and Metarhizium robertsii, commonly used in biological plant prote...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Polish Academy of Sciences
2020-03-01
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| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://doi.org/10.24425/jppr.2020.132207 |
| _version_ | 1798017648650354688 |
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| author | Łukasz Łopusiewicz Kinga Mazurkiewicz-Zapałowicz Cezary Tkaczuk Artur Bartkowiak |
| author_facet | Łukasz Łopusiewicz Kinga Mazurkiewicz-Zapałowicz Cezary Tkaczuk Artur Bartkowiak |
| author_sort | Łukasz Łopusiewicz |
| collection | DOAJ |
| description | This study focused on the effect of heavy metal cobalt ions (at concentrations of
1–1000 ppm) on the development and enzymatic activity of four entomopathogenic fungi:
Beauveria bassiana, Beauveria brongniartii, Isaria fumosorosea and Metarhizium robertsii,
commonly used in biological plant protection. It was found that each of the tested species
of fungi reacted individually to contact with the Co2+ ions at their various concentrations.
Depending on the variants of the experiment carried out, there were changes in the development
of the mycelia (mainly growth inhibition) and their morphological features (color
and structure) in comparison to the control samples. Co2+ ions had a fungistatic effect
on all fungal strains, whereas a fungicidal effect was noted at concentrations of 750 ppm
and 1000 ppm against M. robertsii and I. fumosorosea, respectively. In addition, there was
a discrepancy in enzymatic activity between the tested fungal species developing in the medium
with varying concentrations of metal salt. The inhibitory effect of Co2+ ions on lipase
production was observed in I. fumosorosea. Protease production was stimulated in B. bassiana
at all Co2+ concentrations, whereas in M. robertsii this effect was noted at 1 ppm. The
changing dynamics of extracellular fungal hydrolases, due to the action of Co2+ ions, may
translate into the role of these microorganisms in the processes of insect pathogenesis. This
work suggests that severe pollution of the environment by cobalt could be a restrictive factor
for the development and pathogenicity of entomopathogenic fungi and must be taken
into account for their successful application in biological plant protection. |
| first_indexed | 2024-04-11T16:10:45Z |
| format | Article |
| id | doaj.art-61a9f942f3dc45548bedc6ce2751f0a1 |
| institution | Directory Open Access Journal |
| issn | 1899-007X 1899-007X |
| language | English |
| last_indexed | 2024-04-11T16:10:45Z |
| publishDate | 2020-03-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Journal of Plant Protection Research |
| spelling | doaj.art-61a9f942f3dc45548bedc6ce2751f0a12022-12-22T04:14:41ZengPolish Academy of SciencesJournal of Plant Protection Research1899-007X1899-007X2020-03-01601586710.24425/jppr.2020.132207The influence of cobalt ions on growth and enzymatic activity of entomopathogenic fungi used in biological plant protectionŁukasz Łopusiewicz0https://orcid.org/0000-0001-9499-8366Kinga Mazurkiewicz-Zapałowicz1https://orcid.org/0000-0002-3951-504XCezary Tkaczuk2https://orcid.org/0000-0002-4096-2154Artur Bartkowiak3https://orcid.org/0000-0002-6583-946XCenter of Bioimmobilisation and Innovative Packaging Materials, West Pomeranian University of Technology, Szczecin, PolandDepartment of Hydrobiology, Ichthyology and Biotechnology of Reproduction, West Pomeranian University of Technology, Szczecin, PolandDepartment of Plant Protection and Breeding, Siedlce University of Natural Sciences and Humanities, Siedlce, PolandCenter of Bioimmobilisation and Innovative Packaging Materials, West Pomeranian University of Technology, Szczecin, PolandThis study focused on the effect of heavy metal cobalt ions (at concentrations of 1–1000 ppm) on the development and enzymatic activity of four entomopathogenic fungi: Beauveria bassiana, Beauveria brongniartii, Isaria fumosorosea and Metarhizium robertsii, commonly used in biological plant protection. It was found that each of the tested species of fungi reacted individually to contact with the Co2+ ions at their various concentrations. Depending on the variants of the experiment carried out, there were changes in the development of the mycelia (mainly growth inhibition) and their morphological features (color and structure) in comparison to the control samples. Co2+ ions had a fungistatic effect on all fungal strains, whereas a fungicidal effect was noted at concentrations of 750 ppm and 1000 ppm against M. robertsii and I. fumosorosea, respectively. In addition, there was a discrepancy in enzymatic activity between the tested fungal species developing in the medium with varying concentrations of metal salt. The inhibitory effect of Co2+ ions on lipase production was observed in I. fumosorosea. Protease production was stimulated in B. bassiana at all Co2+ concentrations, whereas in M. robertsii this effect was noted at 1 ppm. The changing dynamics of extracellular fungal hydrolases, due to the action of Co2+ ions, may translate into the role of these microorganisms in the processes of insect pathogenesis. This work suggests that severe pollution of the environment by cobalt could be a restrictive factor for the development and pathogenicity of entomopathogenic fungi and must be taken into account for their successful application in biological plant protection.https://doi.org/10.24425/jppr.2020.132207cobaltentomopathogenic fungienzymatic activityheavy metals |
| spellingShingle | Łukasz Łopusiewicz Kinga Mazurkiewicz-Zapałowicz Cezary Tkaczuk Artur Bartkowiak The influence of cobalt ions on growth and enzymatic activity of entomopathogenic fungi used in biological plant protection Journal of Plant Protection Research cobalt entomopathogenic fungi enzymatic activity heavy metals |
| title | The influence of cobalt ions on growth and enzymatic activity of entomopathogenic fungi used in biological plant protection |
| title_full | The influence of cobalt ions on growth and enzymatic activity of entomopathogenic fungi used in biological plant protection |
| title_fullStr | The influence of cobalt ions on growth and enzymatic activity of entomopathogenic fungi used in biological plant protection |
| title_full_unstemmed | The influence of cobalt ions on growth and enzymatic activity of entomopathogenic fungi used in biological plant protection |
| title_short | The influence of cobalt ions on growth and enzymatic activity of entomopathogenic fungi used in biological plant protection |
| title_sort | influence of cobalt ions on growth and enzymatic activity of entomopathogenic fungi used in biological plant protection |
| topic | cobalt entomopathogenic fungi enzymatic activity heavy metals |
| url | https://doi.org/10.24425/jppr.2020.132207 |
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