Layered PCL scaffolds modified with bioactive additives fabricated by electrospinning and 3D-printing for the nasal bone and cartilage defects
This paper presents a method for fabricating layered materials supporting the reconstruction of nasal tissues by combining the 3D/fused deposition modeling (FDM) technology with electrospinning (ES). Polycaprolactone (PCL) scaffolds modified with bioglass (BG) and zinc (Zn)-doped BG were printed fro...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
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| Series: | Materials & Design |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127523006706 |
| _version_ | 1797671463311900672 |
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| author | Anna Kurowska Anna Nikodem Adam Jabłoński Jarosław Janusz Piotr Szczygieł Magdalena Ziąbka Elżbieta Menaszek Michał Dziadek Barbara Zagrajczuk Magdalena Kobielarz Izabella Rajzer |
| author_facet | Anna Kurowska Anna Nikodem Adam Jabłoński Jarosław Janusz Piotr Szczygieł Magdalena Ziąbka Elżbieta Menaszek Michał Dziadek Barbara Zagrajczuk Magdalena Kobielarz Izabella Rajzer |
| author_sort | Anna Kurowska |
| collection | DOAJ |
| description | This paper presents a method for fabricating layered materials supporting the reconstruction of nasal tissues by combining the 3D/fused deposition modeling (FDM) technology with electrospinning (ES). Polycaprolactone (PCL) scaffolds modified with bioglass (BG) and zinc (Zn)-doped BG were printed from the connectable filaments sticks. Then, using the ES, a nanofibrous membrane with particles of the pharmaceutical drug Osteogenon (OST), was applied directly on a spatial scaffold. The layered scaffolds were investigated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and micro-computed tomography (µCT). The bioactivity and biocompatibility of the scaffolds were evaluated by in vitro studies. The results indicated that the presence of the OST drug in the top layer of scaffolds promoted bioactivity. The presence of a membrane had a positive effect on the production and secretion of aggrecan (Agg), and incorporation of BG particles significantly improved the production and secretion of collagen type II (Col2). Each of the introduced PCL modifications leads to articular cartilage cell (NHAC-kn) viability improvement and results in a decrease in the cytotoxicity of the material. Moreover, in the long-term culture, cell proliferation was positively affected by the introduction of the OST-modified membrane and also by the addition of Zn-doped BG particles. |
| first_indexed | 2024-03-11T21:15:55Z |
| format | Article |
| id | doaj.art-61ac72e00a324c53a83df3aa44230145 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-03-11T21:15:55Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-61ac72e00a324c53a83df3aa442301452023-09-29T04:43:33ZengElsevierMaterials & Design0264-12752023-09-01233112255Layered PCL scaffolds modified with bioactive additives fabricated by electrospinning and 3D-printing for the nasal bone and cartilage defectsAnna Kurowska0Anna Nikodem1Adam Jabłoński2Jarosław Janusz3Piotr Szczygieł4Magdalena Ziąbka5Elżbieta Menaszek6Michał Dziadek7Barbara Zagrajczuk8Magdalena Kobielarz9Izabella Rajzer10Department of Mechanical Engineering Fundamentals, Faculty of Mechanical Engineering and Computer Science, University of Bielsko-Biala, Bielsko-Biała, PolandDepartment of Mechanics, Materials and Biomedical Engineering, Faculty of Mechanical Engineering, Wroclaw University of Science and Technology, Wrocław, PolandDepartment of Mechanical Engineering Fundamentals, Faculty of Mechanical Engineering and Computer Science, University of Bielsko-Biala, Bielsko-Biała, PolandDepartment of Mechanical Engineering Fundamentals, Faculty of Mechanical Engineering and Computer Science, University of Bielsko-Biala, Bielsko-Biała, PolandDepartment of Mechanical Engineering Fundamentals, Faculty of Mechanical Engineering and Computer Science, University of Bielsko-Biala, Bielsko-Biała, PolandDepartment of Ceramics and Refractories, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków, PolandDepartment of Cytobiology, Collegium Medicum, Jagiellonian University, Kraków, PolandDepartment of Glass Technology and Amorphous Coatings, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków, Poland; Faculty of Chemistry, Jagiellonian University, Kraków, PolandDepartment of Glass Technology and Amorphous Coatings, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków, PolandDepartment of Mechanics, Materials and Biomedical Engineering, Faculty of Mechanical Engineering, Wroclaw University of Science and Technology, Wrocław, PolandDepartment of Mechanical Engineering Fundamentals, Faculty of Mechanical Engineering and Computer Science, University of Bielsko-Biala, Bielsko-Biała, Poland; Corresponding author.This paper presents a method for fabricating layered materials supporting the reconstruction of nasal tissues by combining the 3D/fused deposition modeling (FDM) technology with electrospinning (ES). Polycaprolactone (PCL) scaffolds modified with bioglass (BG) and zinc (Zn)-doped BG were printed from the connectable filaments sticks. Then, using the ES, a nanofibrous membrane with particles of the pharmaceutical drug Osteogenon (OST), was applied directly on a spatial scaffold. The layered scaffolds were investigated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and micro-computed tomography (µCT). The bioactivity and biocompatibility of the scaffolds were evaluated by in vitro studies. The results indicated that the presence of the OST drug in the top layer of scaffolds promoted bioactivity. The presence of a membrane had a positive effect on the production and secretion of aggrecan (Agg), and incorporation of BG particles significantly improved the production and secretion of collagen type II (Col2). Each of the introduced PCL modifications leads to articular cartilage cell (NHAC-kn) viability improvement and results in a decrease in the cytotoxicity of the material. Moreover, in the long-term culture, cell proliferation was positively affected by the introduction of the OST-modified membrane and also by the addition of Zn-doped BG particles.http://www.sciencedirect.com/science/article/pii/S0264127523006706Polycaprolactone3D-printingElectrospinningBioglassZn-doped bioglassDrug |
| spellingShingle | Anna Kurowska Anna Nikodem Adam Jabłoński Jarosław Janusz Piotr Szczygieł Magdalena Ziąbka Elżbieta Menaszek Michał Dziadek Barbara Zagrajczuk Magdalena Kobielarz Izabella Rajzer Layered PCL scaffolds modified with bioactive additives fabricated by electrospinning and 3D-printing for the nasal bone and cartilage defects Materials & Design Polycaprolactone 3D-printing Electrospinning Bioglass Zn-doped bioglass Drug |
| title | Layered PCL scaffolds modified with bioactive additives fabricated by electrospinning and 3D-printing for the nasal bone and cartilage defects |
| title_full | Layered PCL scaffolds modified with bioactive additives fabricated by electrospinning and 3D-printing for the nasal bone and cartilage defects |
| title_fullStr | Layered PCL scaffolds modified with bioactive additives fabricated by electrospinning and 3D-printing for the nasal bone and cartilage defects |
| title_full_unstemmed | Layered PCL scaffolds modified with bioactive additives fabricated by electrospinning and 3D-printing for the nasal bone and cartilage defects |
| title_short | Layered PCL scaffolds modified with bioactive additives fabricated by electrospinning and 3D-printing for the nasal bone and cartilage defects |
| title_sort | layered pcl scaffolds modified with bioactive additives fabricated by electrospinning and 3d printing for the nasal bone and cartilage defects |
| topic | Polycaprolactone 3D-printing Electrospinning Bioglass Zn-doped bioglass Drug |
| url | http://www.sciencedirect.com/science/article/pii/S0264127523006706 |
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