HPV related p16INK4A and HSV in benign and potentially malignant oral mucosa pathologies
Abstract Background The association of Human Papilloma Virus (HPV) and Human Syncytial Virus (HSV) infection with inflammatory and potentially malignant disorders of the oral cavity (OPMD) is unknown. The aim of this cross-sectional study was to stablish the expression of the p16INK4A and HSV protei...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | BMC Oral Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12903-024-04105-z |
| _version_ | 1827309928262402048 |
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| author | Irena Duś-Ilnicka Agnieszka Hałoń Andrea Perra Małgorzata Radwan-Oczko |
| author_facet | Irena Duś-Ilnicka Agnieszka Hałoń Andrea Perra Małgorzata Radwan-Oczko |
| author_sort | Irena Duś-Ilnicka |
| collection | DOAJ |
| description | Abstract Background The association of Human Papilloma Virus (HPV) and Human Syncytial Virus (HSV) infection with inflammatory and potentially malignant disorders of the oral cavity (OPMD) is unknown. The aim of this cross-sectional study was to stablish the expression of the p16INK4A and HSV proteins, to test potential correlation between those parameters in biopsies from clinically diagnosed oral lesions. Methods Immunochemical analysis of 211 formalin-fixed, paraffin-embedded (FFPE) blocks from 211 individuals was provided. The clinical diagnosis included in the research were Oral lichen planus (N = 30), Oral Leukoplakia (N = 13) Mucocele (N = 25), Erosion/ulceration/ inflammation of mucosa (N = 8), Overgrowth of mucosa (N = 135). Results Two hundred eleven analyzed FFPE samples resulted with the median age of 58.5 years (the average age 54.0 years and SD ± 17 years). The female/male ratio was 2.3 (69.7% vs 30.3% respectively). All the samples positive for HSV also expressed p16INK4A (p = 0.000), that’s showed various levels of association with the diverse clinical diagnosis reaching the higher level in OM 49.1% (29 positive samples) and OLP 30.5% (18). p16INK4A was associated with OLP at 30.5% (18), and fibroma 30.5%. HSV expression was mostly present in fibroma at 47.6% (10 positive samples). Conclusion HSV and p16INK4A positivity in relation to diagnosis of the biopsies showed statistically most often p16INK4A in OLP and fibroma. The results of co-expression of p16INK4A and HSV in mucocele and fibroma in oral mucosa suggest a cooperation between the molecular alterations induced by these two viruses. Squamous papilloma samples positive for p16INK4A were also positive for HSV, suggesting that the putative pro-oncogenic action of HSV could be an early event. |
| first_indexed | 2024-04-24T19:51:39Z |
| format | Article |
| id | doaj.art-61ad63f37cdb44ee91b168f9b4392e18 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-24T19:51:39Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-61ad63f37cdb44ee91b168f9b4392e182024-03-24T12:35:46ZengBMCBMC Oral Health1472-68312024-03-0124111410.1186/s12903-024-04105-zHPV related p16INK4A and HSV in benign and potentially malignant oral mucosa pathologiesIrena Duś-Ilnicka0Agnieszka Hałoń1Andrea Perra2Małgorzata Radwan-Oczko3Oral Pathology Department, Faculty of Dentistry, Wroclaw Medical UniversityDivision of Clinical Pathology, Department of Clinical and Experimental Pathology, Wroclaw Medical UniversitySection of Pathology, Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria - MonserratoOral Pathology Department, Faculty of Dentistry, Wroclaw Medical UniversityAbstract Background The association of Human Papilloma Virus (HPV) and Human Syncytial Virus (HSV) infection with inflammatory and potentially malignant disorders of the oral cavity (OPMD) is unknown. The aim of this cross-sectional study was to stablish the expression of the p16INK4A and HSV proteins, to test potential correlation between those parameters in biopsies from clinically diagnosed oral lesions. Methods Immunochemical analysis of 211 formalin-fixed, paraffin-embedded (FFPE) blocks from 211 individuals was provided. The clinical diagnosis included in the research were Oral lichen planus (N = 30), Oral Leukoplakia (N = 13) Mucocele (N = 25), Erosion/ulceration/ inflammation of mucosa (N = 8), Overgrowth of mucosa (N = 135). Results Two hundred eleven analyzed FFPE samples resulted with the median age of 58.5 years (the average age 54.0 years and SD ± 17 years). The female/male ratio was 2.3 (69.7% vs 30.3% respectively). All the samples positive for HSV also expressed p16INK4A (p = 0.000), that’s showed various levels of association with the diverse clinical diagnosis reaching the higher level in OM 49.1% (29 positive samples) and OLP 30.5% (18). p16INK4A was associated with OLP at 30.5% (18), and fibroma 30.5%. HSV expression was mostly present in fibroma at 47.6% (10 positive samples). Conclusion HSV and p16INK4A positivity in relation to diagnosis of the biopsies showed statistically most often p16INK4A in OLP and fibroma. The results of co-expression of p16INK4A and HSV in mucocele and fibroma in oral mucosa suggest a cooperation between the molecular alterations induced by these two viruses. Squamous papilloma samples positive for p16INK4A were also positive for HSV, suggesting that the putative pro-oncogenic action of HSV could be an early event.https://doi.org/10.1186/s12903-024-04105-zLichen Planus, OralLeukoplakia, OralHerpesvirus 1HumanHPVOncogenic viruses |
| spellingShingle | Irena Duś-Ilnicka Agnieszka Hałoń Andrea Perra Małgorzata Radwan-Oczko HPV related p16INK4A and HSV in benign and potentially malignant oral mucosa pathologies BMC Oral Health Lichen Planus, Oral Leukoplakia, Oral Herpesvirus 1 Human HPV Oncogenic viruses |
| title | HPV related p16INK4A and HSV in benign and potentially malignant oral mucosa pathologies |
| title_full | HPV related p16INK4A and HSV in benign and potentially malignant oral mucosa pathologies |
| title_fullStr | HPV related p16INK4A and HSV in benign and potentially malignant oral mucosa pathologies |
| title_full_unstemmed | HPV related p16INK4A and HSV in benign and potentially malignant oral mucosa pathologies |
| title_short | HPV related p16INK4A and HSV in benign and potentially malignant oral mucosa pathologies |
| title_sort | hpv related p16ink4a and hsv in benign and potentially malignant oral mucosa pathologies |
| topic | Lichen Planus, Oral Leukoplakia, Oral Herpesvirus 1 Human HPV Oncogenic viruses |
| url | https://doi.org/10.1186/s12903-024-04105-z |
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