In situ Prokaryotic and Eukaryotic Communities on Microplastic Particles in a Small Headwater Stream in Germany
The ubiquitous use of plastic products in our daily life is often accompanied by improper disposal. The first interactions of plastics with organisms in the environment occur by overgrowth or biofilm formation on the particle surface, which can facilitate the ingestion by animals. In order to elucid...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.660024/full |
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| author | Alfons R. Weig Martin G. J. Löder Anja F. R. M. Ramsperger Anja F. R. M. Ramsperger Christian Laforsch |
| author_facet | Alfons R. Weig Martin G. J. Löder Anja F. R. M. Ramsperger Anja F. R. M. Ramsperger Christian Laforsch |
| author_sort | Alfons R. Weig |
| collection | DOAJ |
| description | The ubiquitous use of plastic products in our daily life is often accompanied by improper disposal. The first interactions of plastics with organisms in the environment occur by overgrowth or biofilm formation on the particle surface, which can facilitate the ingestion by animals. In order to elucidate the colonization of plastic particles by prokaryotic and eukaryotic microorganisms in situ, we investigated microbial communities in biofilms on four different polymer types and on mineral particles in a small headwater stream 500 m downstream of a wastewater treatment plant in Germany. Microplastic and mineral particles were exposed to the free-flowing water for 4 weeks in spring and in summer. The microbial composition of the developing biofilm was analyzed by 16S and 18S amplicon sequencing. Despite the expected seasonal differences in the microbial composition of pro- and eukaryotic communities, we repeatedly observed polymer type-specific differentiation in both seasons. The order of polymer type-specific prokaryotic and eukaryotic community distances calculated by Robust Aitchison principal component analysis (PCA) was the same in spring and summer samples. However, the magnitude of the distance differed considerably between polymer types. Prokaryotic communities on polyethylene particles exhibited the most considerable difference to other particles in summer, while eukaryotic communities on polypropylene particles showed the most considerable difference to other spring samples. The most contributing bacterial taxa to the polyethylene-specific differentiation belong to the Planctomycetales, Saccharimonadales, Bryobacterales, uncultured Acidiomicrobia, and Gemmatimonadales. The most remarkable differences in eukaryotic microorganism abundances could be observed in several distinct groups of Ciliophora (ciliates) and Chlorophytes (green algae). Prediction of community functions from taxonomic abundances revealed differences between spring and summer, and – to a lesser extent – also between polymer types and mineral surfaces. Our results show that different microplastic particles were colonized by different biofilm communities. These findings may be used for advanced experimental designs to investigate the role of microorganisms on the fate of microplastic particles in freshwater ecosystems. |
| first_indexed | 2024-12-21T09:59:50Z |
| format | Article |
| id | doaj.art-61b0333264864a60aee17c4143d413b4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T09:59:50Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-61b0333264864a60aee17c4143d413b42022-12-21T19:07:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-11-011210.3389/fmicb.2021.660024660024In situ Prokaryotic and Eukaryotic Communities on Microplastic Particles in a Small Headwater Stream in GermanyAlfons R. Weig0Martin G. J. Löder1Anja F. R. M. Ramsperger2Anja F. R. M. Ramsperger3Christian Laforsch4Genomics and Bioinformatics, Bayreuth Center of Ecology and Environmental Research, University of Bayreuth, Bayreuth, GermanyAnimal Ecology, Bayreuth Center of Ecology and Environmental Research, University of Bayreuth, Bayreuth, GermanyAnimal Ecology, Bayreuth Center of Ecology and Environmental Research, University of Bayreuth, Bayreuth, GermanyBiological Physics, University of Bayreuth, Bayreuth, GermanyAnimal Ecology, Bayreuth Center of Ecology and Environmental Research, University of Bayreuth, Bayreuth, GermanyThe ubiquitous use of plastic products in our daily life is often accompanied by improper disposal. The first interactions of plastics with organisms in the environment occur by overgrowth or biofilm formation on the particle surface, which can facilitate the ingestion by animals. In order to elucidate the colonization of plastic particles by prokaryotic and eukaryotic microorganisms in situ, we investigated microbial communities in biofilms on four different polymer types and on mineral particles in a small headwater stream 500 m downstream of a wastewater treatment plant in Germany. Microplastic and mineral particles were exposed to the free-flowing water for 4 weeks in spring and in summer. The microbial composition of the developing biofilm was analyzed by 16S and 18S amplicon sequencing. Despite the expected seasonal differences in the microbial composition of pro- and eukaryotic communities, we repeatedly observed polymer type-specific differentiation in both seasons. The order of polymer type-specific prokaryotic and eukaryotic community distances calculated by Robust Aitchison principal component analysis (PCA) was the same in spring and summer samples. However, the magnitude of the distance differed considerably between polymer types. Prokaryotic communities on polyethylene particles exhibited the most considerable difference to other particles in summer, while eukaryotic communities on polypropylene particles showed the most considerable difference to other spring samples. The most contributing bacterial taxa to the polyethylene-specific differentiation belong to the Planctomycetales, Saccharimonadales, Bryobacterales, uncultured Acidiomicrobia, and Gemmatimonadales. The most remarkable differences in eukaryotic microorganism abundances could be observed in several distinct groups of Ciliophora (ciliates) and Chlorophytes (green algae). Prediction of community functions from taxonomic abundances revealed differences between spring and summer, and – to a lesser extent – also between polymer types and mineral surfaces. Our results show that different microplastic particles were colonized by different biofilm communities. These findings may be used for advanced experimental designs to investigate the role of microorganisms on the fate of microplastic particles in freshwater ecosystems.https://www.frontiersin.org/articles/10.3389/fmicb.2021.660024/fullbiofilmprokaryote communityeukaryote communityfreshwater streammicroplastics |
| spellingShingle | Alfons R. Weig Martin G. J. Löder Anja F. R. M. Ramsperger Anja F. R. M. Ramsperger Christian Laforsch In situ Prokaryotic and Eukaryotic Communities on Microplastic Particles in a Small Headwater Stream in Germany Frontiers in Microbiology biofilm prokaryote community eukaryote community freshwater stream microplastics |
| title | In situ Prokaryotic and Eukaryotic Communities on Microplastic Particles in a Small Headwater Stream in Germany |
| title_full | In situ Prokaryotic and Eukaryotic Communities on Microplastic Particles in a Small Headwater Stream in Germany |
| title_fullStr | In situ Prokaryotic and Eukaryotic Communities on Microplastic Particles in a Small Headwater Stream in Germany |
| title_full_unstemmed | In situ Prokaryotic and Eukaryotic Communities on Microplastic Particles in a Small Headwater Stream in Germany |
| title_short | In situ Prokaryotic and Eukaryotic Communities on Microplastic Particles in a Small Headwater Stream in Germany |
| title_sort | in situ prokaryotic and eukaryotic communities on microplastic particles in a small headwater stream in germany |
| topic | biofilm prokaryote community eukaryote community freshwater stream microplastics |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.660024/full |
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