Complete Genome sequence of the nematicidal Bacillus thuringiensis MYBT18246
Abstract Bacillus thuringiensis is a rod-shaped facultative anaerobic spore forming bacterium of the genus Bacillus . The defining feature of the species is the ability to produce parasporal crystal inclusion bodies, consisting of δ-endotoxins, encoded by cry-genes. Here we present the complete anno...
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| Format: | Article |
| Language: | English |
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BMC
2017-08-01
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| Series: | Standards in Genomic Sciences |
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| Online Access: | http://link.springer.com/article/10.1186/s40793-017-0259-x |
| _version_ | 1819000952283201536 |
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| author | Jacqueline Hollensteiner Anja Poehlein Cathrin Spröer Boyke Bunk Anna E. Sheppard Philip Rosentstiel Hinrich Schulenburg Heiko Liesegang |
| author_facet | Jacqueline Hollensteiner Anja Poehlein Cathrin Spröer Boyke Bunk Anna E. Sheppard Philip Rosentstiel Hinrich Schulenburg Heiko Liesegang |
| author_sort | Jacqueline Hollensteiner |
| collection | DOAJ |
| description | Abstract Bacillus thuringiensis is a rod-shaped facultative anaerobic spore forming bacterium of the genus Bacillus . The defining feature of the species is the ability to produce parasporal crystal inclusion bodies, consisting of δ-endotoxins, encoded by cry-genes. Here we present the complete annotated genome sequence of the nematicidal B. thuringiensis strain MYBT18246. The genome comprises one 5,867,749 bp chromosome and 11 plasmids which vary in size from 6330 bp to 150,790 bp. The chromosome contains 6092 protein-coding and 150 RNA genes, including 36 rRNA genes. The plasmids encode 997 proteins and 4 t-RNA’s. Analysis of the genome revealed a large number of mobile elements involved in genome plasticity including 11 plasmids and 16 chromosomal prophages. Three different nematicidal toxin genes were identified and classified according to the Cry toxin naming committee as cry13Aa2, cry13Ba1, and cry13Ab1. Strikingly, these genes are located on the chromosome in close proximity to three separate prophages. Moreover, four putative toxin genes of different toxin classes were identified on the plasmids p120510 (Vip-like toxin), p120416 (Cry-like toxin) and p109822 (two Bin-like toxins). A comparative genome analysis of B. thuringiensis MYBT18246 with three closely related B. thuringiensis strains enabled determination of the pan-genome of B. thuringiensis MYBT18246, revealing a large number of singletons, mostly represented by phage genes, morons and cryptic genes. |
| first_indexed | 2024-12-20T22:41:29Z |
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| language | English |
| last_indexed | 2024-12-20T22:41:29Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Standards in Genomic Sciences |
| spelling | doaj.art-61b4888f7a614bea8763858a1b7f346e2022-12-21T19:24:27ZengBMCStandards in Genomic Sciences1944-32772017-08-0112111010.1186/s40793-017-0259-xComplete Genome sequence of the nematicidal Bacillus thuringiensis MYBT18246Jacqueline Hollensteiner0Anja Poehlein1Cathrin Spröer2Boyke Bunk3Anna E. Sheppard4Philip Rosentstiel5Hinrich Schulenburg6Heiko Liesegang7Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, University of GöttingenDepartment of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, University of GöttingenLeibniz Institute DSMZ-German Collection of Microorganisms and Cell CulturesLeibniz Institute DSMZ-German Collection of Microorganisms and Cell CulturesPresent address: Nuffield Department of Medicine, University of OxfordInstitute of Clinical Molecular Biology, Christian-Albrechts University of KielDepartment of Evolutionary Ecology and Genetics, Zoological Institute, Christian-Albrechts University of KielDepartment of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, University of GöttingenAbstract Bacillus thuringiensis is a rod-shaped facultative anaerobic spore forming bacterium of the genus Bacillus . The defining feature of the species is the ability to produce parasporal crystal inclusion bodies, consisting of δ-endotoxins, encoded by cry-genes. Here we present the complete annotated genome sequence of the nematicidal B. thuringiensis strain MYBT18246. The genome comprises one 5,867,749 bp chromosome and 11 plasmids which vary in size from 6330 bp to 150,790 bp. The chromosome contains 6092 protein-coding and 150 RNA genes, including 36 rRNA genes. The plasmids encode 997 proteins and 4 t-RNA’s. Analysis of the genome revealed a large number of mobile elements involved in genome plasticity including 11 plasmids and 16 chromosomal prophages. Three different nematicidal toxin genes were identified and classified according to the Cry toxin naming committee as cry13Aa2, cry13Ba1, and cry13Ab1. Strikingly, these genes are located on the chromosome in close proximity to three separate prophages. Moreover, four putative toxin genes of different toxin classes were identified on the plasmids p120510 (Vip-like toxin), p120416 (Cry-like toxin) and p109822 (two Bin-like toxins). A comparative genome analysis of B. thuringiensis MYBT18246 with three closely related B. thuringiensis strains enabled determination of the pan-genome of B. thuringiensis MYBT18246, revealing a large number of singletons, mostly represented by phage genes, morons and cryptic genes.http://link.springer.com/article/10.1186/s40793-017-0259-xBacillus thuringiensisBacillus cereus sensu latoProphagesParasporal crystal proteinPan-Core-genome |
| spellingShingle | Jacqueline Hollensteiner Anja Poehlein Cathrin Spröer Boyke Bunk Anna E. Sheppard Philip Rosentstiel Hinrich Schulenburg Heiko Liesegang Complete Genome sequence of the nematicidal Bacillus thuringiensis MYBT18246 Standards in Genomic Sciences Bacillus thuringiensis Bacillus cereus sensu lato Prophages Parasporal crystal protein Pan-Core-genome |
| title | Complete Genome sequence of the nematicidal Bacillus thuringiensis MYBT18246 |
| title_full | Complete Genome sequence of the nematicidal Bacillus thuringiensis MYBT18246 |
| title_fullStr | Complete Genome sequence of the nematicidal Bacillus thuringiensis MYBT18246 |
| title_full_unstemmed | Complete Genome sequence of the nematicidal Bacillus thuringiensis MYBT18246 |
| title_short | Complete Genome sequence of the nematicidal Bacillus thuringiensis MYBT18246 |
| title_sort | complete genome sequence of the nematicidal bacillus thuringiensis mybt18246 |
| topic | Bacillus thuringiensis Bacillus cereus sensu lato Prophages Parasporal crystal protein Pan-Core-genome |
| url | http://link.springer.com/article/10.1186/s40793-017-0259-x |
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