Antibiotic susceptibility pattern of Portuguese environmental Legionella isolates
IntroductionLegionnaires’ Disease is a pneumonia caused by Legionella spp., currently treated empirically with fluoroquinolones and macrolides. In this study, we aim to describe the antibiotic susceptibility pattern of environmental Legionella recovered in the south of Portugal.MethodsMinimal inhibi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-04-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1141115/full |
| _version_ | 1797679155632930816 |
|---|---|
| author | Carolina Cruz Lúcia Rodrigues Filipa Fernandes Ricardo Santos Paulo Paixão Maria Jesus Chasqueira |
| author_facet | Carolina Cruz Lúcia Rodrigues Filipa Fernandes Ricardo Santos Paulo Paixão Maria Jesus Chasqueira |
| author_sort | Carolina Cruz |
| collection | DOAJ |
| description | IntroductionLegionnaires’ Disease is a pneumonia caused by Legionella spp., currently treated empirically with fluoroquinolones and macrolides. In this study, we aim to describe the antibiotic susceptibility pattern of environmental Legionella recovered in the south of Portugal.MethodsMinimal inhibitory concentration (MIC) determination of 57 Legionella isolates (10 Lp sg 1, 32, Lp sg 2-14 15 L. spp) was achieved by broth microdilution, as described by EUCAST, for azithromycin, clarithromycin, ciprofloxacin, levofloxacin, and doxycycline. ResultsFluoroquinolones were the most active antibiotic, displaying the lowest MIC values in contrast to doxycycline which had the highest. MIC90 and epidemiological cut-off (ECOFF) values were, respectively, 0.5/1 mg/L for azithromycin, 0.125/0.25 mg/L for clarithromycin, 0.064/0.125 mg/L for ciprofloxacin, 0.125/0.125 mg/L for levofloxacin and 16/32 mg/L for doxycycline.DiscussionMIC distributions were higher than reported by EUCAST for all antibiotics. Interestingly, two phenotypically resistant isolates with high-level quinolone resistance were identified. This is the first time that MIC distributions, lpeAB and tet56 genes have been investigated in Portuguese environmental isolates of Legionella. |
| first_indexed | 2024-03-11T23:10:24Z |
| format | Article |
| id | doaj.art-61b4d9fb17c34996812ae82666361c65 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-11T23:10:24Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-61b4d9fb17c34996812ae82666361c652023-09-21T08:56:05ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-04-011310.3389/fcimb.2023.11411151141115Antibiotic susceptibility pattern of Portuguese environmental Legionella isolatesCarolina Cruz0Lúcia Rodrigues1Filipa Fernandes2Ricardo Santos3Paulo Paixão4Maria Jesus Chasqueira5NOVA Medical School, Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Lisboa, PortugalComprehensive Health Research Center, NOVA Medical School, Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Lisboa, PortugalLaboratório de Análises de Água, Técnico Lisboa, Universidade de Lisboa, Lisboa, PortugalLaboratório de Análises de Água, Técnico Lisboa, Universidade de Lisboa, Lisboa, PortugalComprehensive Health Research Center, NOVA Medical School, Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Lisboa, PortugalComprehensive Health Research Center, NOVA Medical School, Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Lisboa, PortugalIntroductionLegionnaires’ Disease is a pneumonia caused by Legionella spp., currently treated empirically with fluoroquinolones and macrolides. In this study, we aim to describe the antibiotic susceptibility pattern of environmental Legionella recovered in the south of Portugal.MethodsMinimal inhibitory concentration (MIC) determination of 57 Legionella isolates (10 Lp sg 1, 32, Lp sg 2-14 15 L. spp) was achieved by broth microdilution, as described by EUCAST, for azithromycin, clarithromycin, ciprofloxacin, levofloxacin, and doxycycline. ResultsFluoroquinolones were the most active antibiotic, displaying the lowest MIC values in contrast to doxycycline which had the highest. MIC90 and epidemiological cut-off (ECOFF) values were, respectively, 0.5/1 mg/L for azithromycin, 0.125/0.25 mg/L for clarithromycin, 0.064/0.125 mg/L for ciprofloxacin, 0.125/0.125 mg/L for levofloxacin and 16/32 mg/L for doxycycline.DiscussionMIC distributions were higher than reported by EUCAST for all antibiotics. Interestingly, two phenotypically resistant isolates with high-level quinolone resistance were identified. This is the first time that MIC distributions, lpeAB and tet56 genes have been investigated in Portuguese environmental isolates of Legionella. https://www.frontiersin.org/articles/10.3389/fcimb.2023.1141115/fullLegionellabroth microdilutionMICfluoroquinolonesmacrolidesenvironmental |
| spellingShingle | Carolina Cruz Lúcia Rodrigues Filipa Fernandes Ricardo Santos Paulo Paixão Maria Jesus Chasqueira Antibiotic susceptibility pattern of Portuguese environmental Legionella isolates Frontiers in Cellular and Infection Microbiology Legionella broth microdilution MIC fluoroquinolones macrolides environmental |
| title | Antibiotic susceptibility pattern of Portuguese environmental Legionella isolates |
| title_full | Antibiotic susceptibility pattern of Portuguese environmental Legionella isolates |
| title_fullStr | Antibiotic susceptibility pattern of Portuguese environmental Legionella isolates |
| title_full_unstemmed | Antibiotic susceptibility pattern of Portuguese environmental Legionella isolates |
| title_short | Antibiotic susceptibility pattern of Portuguese environmental Legionella isolates |
| title_sort | antibiotic susceptibility pattern of portuguese environmental legionella isolates |
| topic | Legionella broth microdilution MIC fluoroquinolones macrolides environmental |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1141115/full |
| work_keys_str_mv | AT carolinacruz antibioticsusceptibilitypatternofportugueseenvironmentallegionellaisolates AT luciarodrigues antibioticsusceptibilitypatternofportugueseenvironmentallegionellaisolates AT filipafernandes antibioticsusceptibilitypatternofportugueseenvironmentallegionellaisolates AT ricardosantos antibioticsusceptibilitypatternofportugueseenvironmentallegionellaisolates AT paulopaixao antibioticsusceptibilitypatternofportugueseenvironmentallegionellaisolates AT mariajesuschasqueira antibioticsusceptibilitypatternofportugueseenvironmentallegionellaisolates |